JaLCDOI 10.18926/11769
Title Alternative The Study of Nursing Care of Dying Patients and Their Families - Ritual Behaviors and Wordings of Nurses -
FullText URL 001_051_060.pdf
Author Takata, Setuko|
Abstract The purpose of this study was to obtain the basic data for the study of "Nursing care of dying patients and their families". I investigated 59 nurses working at hospitals in Tokushima know their ritual behaviors and their wordings that nurses and their families made for dying patients at moment of their death in the hospital. The results were; 1) Typically, two ritual behaviors were reported at attending patient's death. One was "Tamayobi"; calling dying patient's name to commend his soul to God or Hotoke. Other was "Matugo no mizu", giving water to dying patient to sympathy him. Few nurses didn't know "Tamayobi". Many nurses knew "Mstugo no mizu", however, only a few nurses did it in the hospital. 2) Nurses talked with their families in many ways around their last hours. This wordings included not only to explain dying patient's care, but also to alleviat the grief of their families much more.
Keywords Tamayobi Matugo no mizu
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 51
End Page 60
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313482
JaLCDOI 10.18926/11770
Title Alternative A study on students' idears about the school syllabus
FullText URL 007_1_029_033.pdf
Author Ohta, Takeo| Shimoishi, Yasuaki| Azuma, Yoshiharu| Ichimura, Mituko| Endo, Hiroshi| Okazaki, Yuka|
Abstract 1995年4月に初めての本学のシラバス(講義要覧)が発行され、学生に配布された。本報は約1年後の1996年2月の試験期間中にシラバスに対する学生の意見を問うために行われたアンケート調査の結果をまとめたものである。回答を寄せた学生は435人で、在籍学生の89.0%であった。よく利用したとする学生は18.2%、数回利用したとする学生は67.6%であった。学生の使用目的はテストの準備や履修科目の決定のためであった。以上のことから、現在のシラバスは学生が日常的により活用するためにはさらに教師が改善、工夫する必要があると思われた。
Keywords シラバス (syllabus) 授業計画 講義要覧 学生の意見 (student) use
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 29
End Page 33
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313997
JaLCDOI 10.18926/11771
Title Alternative Factors Affecting Home Care of Elders Report 1. Support Expected by the Elderly and Family
FullText URL 001_061_068.pdf
Author Ono, Tsuruko| Arakawa, Yasuko| Ohta, Niwa| Nanba, Jun| Ohta, Takeo| Shimizu, Masako| Ishii, Kiyoko|
Abstract For the rapid increase of the elders in population in Japan after the 2nd world war, the arrangement of medical care for aged persons, both therapeutic and preventive, has become the serious social problem. To estimate the possibility of care at home and needs of elders and their family for public and volunteer services, questionnaire survey was carried out at Konko town in Okayama prefecture, Japan, in August 1990. More than half of the 105 elders older than 65 years and living alone (group A, 9 males and 96 females) replied to want to be cared for at home and 178 families with elders (group B) wanted to care for them at home. As for services they would wish to be offered when they would become bedridden at home, while group A wished to be visited and cared for by 'physician', 'home helper' and 'neighbor' in order of high rate group B 'physician', 'public health nurse' and 'nurse'. As for facility or assistance services, the former wanted 'purchasing, sweeping and washing', food delivery' and 'calling on' and the latter 'care consultation', 'assist of body bath' and 'economic aid'. Other supports or cares were also hopefully expected by both groups. The results shows that well-arrangement and promotion of a variety of constitional and personal support for home cares for elders in the community must be urged.
Keywords Home Care Family Care the Elderly Support
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 61
End Page 68
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313467
JaLCDOI 10.18926/11772
Title Alternative The influence on the family caused by the mother staying away from home in order to take care of a sick child being hospitalized -six cases of siblings left at home who refused to go to school because of this situation-
FullText URL 007_1_035_039.pdf
Author Ohta, Niwa|
Abstract 母親が病気の子どもに1か月以上付き添う場合の家に残されている同胞への影響について研究を行ってきた。今回、その同胞172ケースのうちで登校拒否があった6ケースについて子どもの特徴、家族構成、家族状況等を検討した。その結果、6ケースのうち3ケースが9歳の女児であった。同居家族は1ケースで、他は核家族であった。この同胞たちは、「笑顔がなくなる」、「寂しそうな表情をする」など何らかの精神的不安を表していた。登校拒否の始まった時期は全て入院後1か月未満の時であった。登校拒否が一時的でなく、退院後にも長びいたケースは父親と女児のみが家に残されていた。このような結果から核家族が多い現在小児看護を行う場合、残された同胞への配慮を早期から行いながら病児や母親の援助を行うことが重要である。
Keywords 付き添い 長期入院 (hospitalization) 家族状況 (mother, family) 同胞 登校拒否 (refusing to go to school)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 35
End Page 39
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313617
JaLCDOI 10.18926/11773
FullText URL 001_069_076.pdf
Author Sasaki Kenji| Hirota Takashi| Iwado Akimasa| Hirota Kazuhiro|
Abstract Reactions of cyclic ketones such as α-tetralone, 1,3-cyclohexanedione, or naphthalenedione with formamide or trisformylaminomethane (TFAM) have shown to form polyclic fused pyrimidines by us. Reactions of terpene ketones like l-menthone, d-camphor, l-carvone with TFAM were performed, and 8-isopropyl-5-methyl-5,6,7,8-tetrahydroquinazoline, borno[2,3-d] pyrimidine, and 5-isopropenyl-8-methyl-5,6-dihydroquinazoline were expectedly obtained from three terpenes. Minor products of 5-isopropenyl-8-methyl-5,6,7,8-tetrahydroquinazoline and 5-isopropenyl-8-methylquinazoline were formed with 5-isopropenyl-8-methyl-5,6-dihydroquinazoline by disproportionation reaction of l-carvone. Furthermore, No-formylmenthylamine, N-formylbornylamine, and N-formylcarvylamine were obtained as the Leuckart-type products terpene ketones in these reactions. The reaction of N-benzyl-4-piperidone with TFAM gave desired 6-benzyl-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine. The reaction of diethyl succinylsuccinate with TFAM afforded tricyclic 4,9-dioxo-3,4,8,9-tetrahydropyrimido[4,5-g]quinazoline. Above compounds were determined by the measurements of their instrumental analyses.
Keywords Cyclic ketone Trisformylaminomethane Fused pyrimidine Cyclization Reductive amination
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 69
End Page 76
ISSN 0917-4494
language English
File Version publisher
NAID 120002313993
JaLCDOI 10.18926/11774
Title Alternative The health and daily-life problems of the elderly living in their own homes for six months after being discharged from the hospital
FullText URL 007_1_041_047.pdf
Author Ohta, Niwa| Inoshita, Hikari| Ikeda, Toshiko| Nakanishi, Yoshiko| Kondo, Masuko| Takata, Setuko| Kojima, Mishako|
Abstract 病院を退院した高齢者の療養上の問題を把握することは、退院指導や継続看護を進める上でも重要である。著者らは退院時、退院後3か月、6か月、1年のそれぞれの時点で、在宅での看護上の問題について調査研究を行い、すでにこれらの一部は報告した。今回は退院後6ヵ月を経過した高齢者の在宅療養時の健康及び日常生活の問題について報告する。総合病院に入院していた70歳以上の高齢者で、退院時に調査を行った93名のうち、退院後6ヵ月の時点で回答を得た60名(64.5%)について分析した。調査は郵送法により、調査内容は健康状態、自立度、困っていること等で、結果は退院時の状態と比較・検討した。退院6か月後の日常生活の自立度がよい者の割合は退院時より8.4%増加し、体の調子が「良くなった」とする者は50%を越えた。しかし、困っていることとして「健康面」の問題をあげた者は23.3%と高く、退院時の2倍になっていた。また退院時の問題点の70%はなお持続しており、食事療法・移動動作・下肢痛・視聴覚障害・排泄障害・気管支炎等の順に多かった。6ヶ月後の新たな問題として注目すべきものとしては人間関係やコミュニケションの障害が認められた。
Keywords 高齢者 (the elderly) 自宅退院 6か月後 (six months after discharge from the hospital) 健康問題 (health) 日常生活 (daily-life problems)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 41
End Page 47
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313479
JaLCDOI 10.18926/11776
Title Alternative Coping for the better quality of life(QOL) after adult kidney transplantation and factors affecting on the coping
FullText URL 007_1_049_057.pdf
Author Hayashi, Yuko|
Abstract 腎移植は、免疫抑制剤の進歩に伴う高い生存率や生着率はもちろんのこと、今日では特に移植後のQOLに焦点が当てられている。移植後のQOLを向上させていくためには、レシピエントが移植後の様々な出来事にいかに対処していくかが重要である。本研究は、腎移植後のレシピエントの対処に焦点を当て、移植後のQOLに関係する対処および対処に影響を及ぼす要因を明らかにすることを目的とした。対象者は、関東の2施設で腎移植を受けて入院および外来通院をしている20才以上の10名のレシピエントで、研究参加の同意を得た後、腎移植後どのように対処しているかや、何が対処に影響を与えているかについて面接並びに観察による調査を行った。分析の結果、腎移植後の対処は<問題と取り組む><情報の交換><問題状況の再認知>である対処様式が主に用いられており、医師に任せるという<お任せ>は全員に見られなかった。また、腎移植後の対処に影響を及ぼす要因として、<身体の状態><自己概念><不確かさ><ソーシャルサポート>が見いだされた。
Keywords 対処 (coping) 身体の状態 (physical state) 自己概念 (self concept) 不確かさ (uncertainty) ソーシャルサポート (social support)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 49
End Page 57
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313679
JaLCDOI 10.18926/11777
Title Alternative Daily physical activity in patients with chronic glomerulonephritis
FullText URL 007_1_059_066.pdf
Author Sato, Yoshie| Takahashi, Kayo| Suzuki, Hisao| Ikeda, Toshiko| Kondo, Masuko|
Abstract 腎炎患者の日常生活活動強度と持久性体力を明らかにするため、本研究を行った。被検者は20歳から61歳の腎炎患者7名である。日常生活活動強度の指標として、連続心拍計を用いて24時間心拍数を測定した。また、持久性体力を評価するため、換気性閾値(Ventilatory Threshold : VT)を測定した。各被験者のVTは、同性、同年齢の健常者の値に対する相対値(%VT)で評価した。被検者の%VTの平均は、93.4±18.6%で、健常者に比べて低下していた。24時間心拍数の平均は、82±8拍/分であった。24時間心拍数のうち、VT時の心拍数を越えるものは、多くても2%でしかなく、腎炎患者の日常生活の大部分は、有酸素運動であった。
Keywords 腎炎 (glomerulonephritis) 日常生活 (daily physical activity) 心拍数 (heart rate) 運動 (physical fitness) 換気性閾値 (ventilatory threshold)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 59
End Page 66
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313866
JaLCDOI 10.18926/11778
Title Alternative Determination of Hematinic Acid Produced by Oxidative Cleavage of Hemoglobin Heme in Red Blood Cells
FullText URL 001_077_082.pdf
Author Hirota, Kazuhiro| Sasaki, Kenji| Hirota, Takashi|
Abstract Our previous studies on the mechanism of phenylhydrazine-induced hemolytic anemia have shown that hematinic acid, one of oxidative cleavage products of heme, is formed by the reaction of hemoglobin with phenylhydrazine. Develoment of the determination of hematinic acid formed by this reaction in red blood cells (RBC) was required to study the mechanism of the hemolysis. Hemolysates prepared by lysis of fresh human RBC with water was mixed with standard hematinic acid. A solution consisting of hydrochloric acid, methanol, and acetone was added, and most of hemoglobin precipitated was removed by centrifugation. Hematinic acid was derived to the methyl ester by incubation with methanol containing sulfuric acid. The ester was passed to two type of silica gel column to remove interferences, and was analysed on a reversedphase high-performance liquid chromatographic column. Hematinic acid could be determined in the range 1.0-20.0μmol/ml RBC. Recovery from hemolysate was 65.0% ±3.5%. Standard compounds of hematinic acid and its methyl ester were prepared by the oxidation of hemin with hydrogen peroxide, and were comfirmed by elemental analyses and mass spectra.
Keywords hematinic acid high-performance liquid chromatography red blood cells heme
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 77
End Page 82
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313412
JaLCDOI 10.18926/11779
Title Alternative An approach to English culture : in the case of Lawrence Country depicted in Lady Chatterley's Lover
FullText URL 007_1_067_074.pdf
Author Takeda, Terufumi|
Abstract 世界に先駆けて産業革命を成し遂げ七つの海を支配した英国の近代機械・産業文明の発展とは反比例するかのように破壊され消滅していく自然の森林と田園。それはかつて英国の中部一帯に広がっていたロビン・フッドの活躍したシャーウッドの森とて例外ではない。そこで現在わずかに残存しているその森の一部を含めてロレンス自身が「わが心のふるさと」と呼んだ生まれ故郷イーストウッドが、彼の最後の小説『チャタリー夫人の恋人』の中にどのように採り入れられ、どのように描写されているかを考察することにより、英国文化の偉大なる伝統が如何なるものであるかのその一端なりを論じてみる。
Keywords 英国文化 (English culture) 『チャタリー夫人の恋人』 (Lady Chatterley's Lover) D.H. ロレンスの故郷 (D.H. Lawrence Country) イーストウッド (Eastwood) 古き良き時代 (the good old days)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 67
End Page 74
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313931
JaLCDOI 10.18926/11780
FullText URL 001_083_089.pdf
Author Yamamoto Hideki| Miyake Hideaki| Takahashi Yasuji| Sumimoto Tetsuhiro|
Abstract Various flow visualization techniques were applied to study the tidal exchange of the Seto Inland Sea with the largest hydraulic model in a horizontal scale 1/2000. To analyze dyefront patterns, image processing techniques have been developed. The techniques include the extraction of the boundary of the dyefront and the flow direction through tuft images.
Keywords Tuft Flow Visualization Dye Visualization Seto Inland Sea Hydraulic Model
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 83
End Page 89
ISSN 0917-4494
language English
File Version publisher
NAID 120002313645
JaLCDOI 10.18926/11781
Title Alternative Time needed for practice of the conduct of labor
FullText URL 007_1_075_080.pdf
Author Goda, Noriko| Shirai, Kiyoko| Okazaki, Yuka|
Abstract 近年の急激な少子・高齢化による人口構造の歪みは、益々の出生率の低下を来たしている。この現状の中での分娩介助実習は非常に厳しい状況にある。そのため、当専攻科では平成元年(1989年)より分娩介助実習場所を2施設から3施設として分娩介助実習に対応している。7年間の平均をみると、学生1人当たり10.7回の分娩介助に要する時間は476時間にのぼる。このうち、正規の実習時間外における拘束時間は184時間であった。
Keywords 助産学 (midwifery) 分娩介助実習 (practice of the conduct of labor) 助産婦教育 (midwifery education)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 75
End Page 80
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313562
JaLCDOI 10.18926/11782
Title Alternative Factors involved in the movement of the center of mass in healthy women
FullText URL 007_1_081_086.pdf
Author Shirai, Kiyoko| Goda, Noriko| Okazaki, Yuka| Jin, Fumio| Ohta, Takeo|
Abstract 妊娠中の体形変化は姿勢変化をもたらし、このためにおこる重心移動がマイナートラブルを起こす原因となることが考えられる。そこで、健康女性37人を対象に、重心動揺を起こす因子と考えられる体型要因に着目した基礎的研究を行い、以下の結果を得た。1)重心動揺測定項目の中で、重心動揺距離、包絡面積、重心動揺面積の項目は開眼時より閉眼時が有意に数値が大きく、X軸方向動揺平均、Y軸方向動揺平均の項目は、開眼時と閉眼時とで差がなかった。2)ロンベルグ姿勢での開眼及び閉眼重心動揺検査では、座高、下肢長に正の相関が、肥満度に負の相関があるこが示唆された。
Keywords 重心動揺 (Sway of the center of gravity) 直立姿勢 (upright standing position) 健康女性 (healthy females) 体型 (body build)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 81
End Page 86
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313349
JaLCDOI 10.18926/11783
Title Alternative The Neurophysiological Implications of an Atypical Slow Negative Potential in Short Interval CNV Paradigm
FullText URL 001_091_097.pdf
Author Okamoto, Motoi| Temino, Masato| Nakagawa, Naohisa| Mori, Hirokazu|
Abstract We recorded a slow negative potential from Cz (10/20 method) in 49 healthy students (12 male, 37 female, mean age 19.1) by a short interstumulus interval CNV paradigm. The interstimulus interval was 2 or 3 seconds, the warning stimulus presented at random or regular interval at 0.2 Hz. An atypical negative variation with two separated negative peaks was recorded in 26.0-30.6% trials regardless of interstimulus interval or modality of warning stimulus presentation, while a typical CNV was recorded in 32.0-59.2% of trials. No apparent negative variation was recorded in 14.3-18.4% in 2 seconds interstimulus interval, and 28-38% in 3 seconds interstimulus interval, showing that 2 seconds interval is better to get stable CNV recording than 3 seconds interval. The first negative wave of the atypical negative variation was 692-799msec in duration, but frequently prolonged to 1000msec or more in 3 seconds interval. It usually had negative peak around 900-1100msec, but sometimes around 1500msec. This features are different from any reported negative components of CNV. The second negative wave began 800-1200msec before second stimulus, and had its peak just before second stimulus, showing common features with readiness potential. The appearance of CNV was unsatble in the students in which the atypical negative variation was recorded in regular, 2 seconds intersitimulus interval, and the amplitude of slow vertex response and pattern reversal visual evoked potential was lower in thses students than in the students in which a typical CNV was recorded more than 3 times in total 4 times of trials. These findings indicate that the atypical variation observed in this study is due to a lowered arousal level or cortical neuronal activity, rather than a separated appearance of different components of CNV.
Keywords contingent negative variation atypical slow negative potential arousal level slow vertex response visual evoked potential
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 91
End Page 97
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313945
JaLCDOI 10.18926/11784
Title Alternative The way to a Bachelor's degree : state of graduates from advanced course of midwifery, School of Health Sciences Okayama University
FullText URL 007_1_087_092.pdf
Author Okazaki, Yuka| Goda, Noriko| Shirai, Kiyoko|
Abstract 平成4年に岡山大学医療技術短期大学部専攻科助産学特別専攻は学位授与機構に認定された。5年目を迎えた今年、現在までを見直すために、平成4年度から平成7年度までの入学生80名のうち、短期大学部の卒業生51名を対象とし、アンケート調査を実施した。その結果以下のことがわかった。学位授与機構への申請経験者は6名(11.8%)で、その内2名は現在申請中である。2名が合格し、看護学士の学位を取得していた。現在取得に向けて学修中の者は17名(33.3%)で、学修成果の作成に関して困っている者が多かった。取得の意志はあるが休止中の者は25名(49%)で、主な理由は仕事が忙しいことであった。取得の意志がない者は7名(13.7%)であった。
Keywords 学士の学位 (Bachelor's degree) 学位授与機構 (National Institution for Academic Degrees) 専攻科助産学特別専攻 (advanced course of midwifery) 大学の単位 (credits) 学修成果 (results of learning and training)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 87
End Page 92
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313882
JaLCDOI 10.18926/11786
Title Alternative Malignant diseases maifesting jaundice as a main symptom -Diagnosis and treatment to improve the poor survival in carcinoma of the bile duct and pancreatic head -
FullText URL 002_001_022.pdf
Author Mimura, Hisashi|
Abstract Primary malignant diseases manifesting jaundice as a main symptom are the carcinomas of the bile duct and the pancreatic head, both of which have very poor survival rates after surgical resection or other treatments. Statistically, unresectable cases of the diseases are absolutely fatal within one or two years. Pathologically curative resection in early stage could only give the patients a chance to survive without diseases. Role of early detection of the diseases at the primary clinics of internal physicians is essentially important, because the patients noticing jaundice would firstly visit them under the impression of having the liver deseases. They should not waste much time in examining various liver function tests. Image diagnosis on the bile duct and the pancreatic duct by ultrasonography, followed by PTC or ERCP, detect the diseases. Tumor marker, CT, MRI and angiography are also performed supplementally to determine the tumor expansion or resectability. Surgery should be the histologically curative resection, including extended skeletonization of the retroperitoneum. Recent five years' survival rates of the bile duct carcinoma after resection in all over Japan are 64.5% in stage-Ⅰ, 50.4% in stage-Ⅱ, 30.7% in stage-Ⅲ and 9.4% in stage-Ⅳ. And those of the pancreatic carcinoma are 39.3% in T(1) (within 2cm in tumor diameter), 21.3% in T(2) (2-4cm), 13.1% in T(3) (4-6cm) and 12.1% in T(4) (over 6cm). From these data, curative resection in the early stages (stage-Ⅰ or -Ⅱ in bile duct carcinoma ant T(1) or T(2) in pancreatic carcinoma) is recommended, but even in the stage-Ⅲ the chance to live longer by extended resection is preserved. To achieve furhter improvement, author has devised the NEW surgical technigues, "block resection of the hepatoduodenal ligament" for carcinoma of the bile duct and "isolated pancreatectomy" for pancreatic carcinoma. Curative resectabilities are increasing by applying these techniques and hepatic recurrence rate in pancreatic carcinoma is decreasing by "isolated pancreatectomy", although the statistical results of improvement in five years' survival rates are not yet achieved because of shortage in cases' numbers and time duration.
Keywords 黄疸 診断 治療 手術
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1992-02-05
Volume volume2
Start Page 1
End Page 22
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313384
Author 岡山大学医療技術短期大学部|
Published Date 1991-03-25
Publication Title 岡山大学医療技術短期大学部紀要
Volume volume1
Content Type Others
Author 岡山大学医療技術短期大学部|
Published Date 1992-02-05
Publication Title 岡山大学医療技術短期大学部紀要
Volume volume2
Content Type Others
JaLCDOI 10.18926/11789
Title Alternative Enzyme Immunoassay for Rabbit C-reactive Protein
FullText URL 002_023_028.pdf
Author Mori, Shuji|
Abstract Sensitive enzyme Immunoassay method specific for rabbit C-reactive protein was established. This was based upon specific Ca(2+)-dependent binding profile of C-reactive protein for some compounds containing phosphorylcholine moiety intramolecularly. By this method, more than 0.001μg of rabbit C-reactive protein was detectable. Specific binding of C-reactive protein for p-aminophenylphosphorylcholine immobilized on solid phase was inhibited by either addition of EDTA or phophorylcholine analogues, effectively. It may be useful to study possible roles of C-reactive protein in inflammatory regions.
Keywords C-reactive Protein Enzyme Immunoassay
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1992-02-05
Volume volume2
Start Page 23
End Page 28
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313528
JaLCDOI 10.18926/11730
Title Alternative Measurement of intracellular pH by flow cytometry using pH sensitive fluorescence dye, and influence of hyperthermia and amiloride derivatives on the intracellular pH
FullText URL 006_001_005.pdf
Author Asaumi, Jun-ichi| Kawasaki, Shoji| Kuroda, Masahiro| Takeda, Yoshihiro| Hiraki, Yoshio|
Abstract エールリッヒ腹水癌細胞とそのアドリアマイシン耐性細胞において蛍光pH指示薬2'、7'-bis-(2-carboxyethyl) carboxyfluorescein] (BCECF) の蛍光量をフローサイトメトリーで測定することによって細胞内pHの検量曲線を作成することができた。このことより、これらの細胞においてBCECFの蛍光量で細胞内pHの変化を簡易に比較できることを示唆した。さらに、温熱、Na(+)/H(+) exchanger の阻害例であるアミロライド[3,5-diamino-6-chloro-N-(diaminomethylene) pyrazinecarboxamide]、およびアミロライド誘導隊MH-12-43[N-amidino-3-amino-6-chloro-5-(N-ethyliso-propylamino) pyrazinecarboxyamide] の細胞内pHへの影響をエールリッヒ腹水癌細胞で観察した。37℃では、0.5mMアミロライド、0.05mM MH-12-43により細胞内pHは減少し、42℃処理によりさらに減少した。42℃において、0.05mM MH-12-43による細胞内pHの減少は、0.5mMアミロライドによる減少より大きかった。
Keywords BCECF 細胞内pH (Intracellular pH) フローサイトメトリー (Flow Cytometry) アミロライド (Amiloride) MH-12-43
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-02-29
Volume volume6
Start Page 1
End Page 5
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313855