Author Ishikawa, Takaki| Miyaishi, Satoru| Doi, Yusuke| Takata, Tomoyo| Imabayashi, Kiyomi| Inagaki, Sachiyo| Yoshitome, Kei| Yamamoto, Yuji| Ishizu, Hideo|
Published Date 2003-01-31
Publication Title 岡山医学会雑誌
Volume volume114
Issue issue3
Content Type Journal Article
Author Ishikawa, Takaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Inagaki, Sachiyo| Okamura, Michihiko| Ishizu, Hideo|
Published Date 2001-12-31
Publication Title 岡山医学会雑誌
Volume volume113
Issue issue3
Content Type Journal Article
Author Ishizu, Hideo| Miyaishi, Satoru| Yamamoto, Yuji| Takata, Shingo|
Published Date 1993-10
Publication Title 岡山医学会雑誌
Volume volume105
Issue issue9-10
Content Type Journal Article
Author Miyaishi, Satoru| Kitao, Takashi| Moriya, Fumio| Yamamoto, Yuji| Ishizu, Hideo| Ishii, Hiroyuki|
Published Date 1991-10
Publication Title 岡山医学会雑誌
Volume volume103
Issue issue9-10
Content Type Journal Article
Author Yamamoto, Yuji|
Published Date 1985-10-30
Publication Title 岡山医学会雑誌
Volume volume97
Issue issue9-10
Content Type Journal Article
JaLCDOI 10.18926/AMO/30512
FullText URL fulltext.pdf
Author Takata, Shingo| Yamamoto, Yuji| Ishizu, Hideo|
Abstract <p>A method of genotyping IgA2 alleles in the human immunoglobulin alpha 2 heavy chain constant region (C alpha 2 gene) was developed by using the polymerase chain reaction (PCR). By this method, the genotype was determined by discriminating base substitution in the 3'-flanking region of alleles, A2m*1 and A2m*2, which manifest A2m serum types, by nested PCR using allele-specific primers. Three types, IgA2*1/IgA2*1, IgA2*2/IgA2*1, and IgA2*2/IgA2*2, were detected from DNA extracted from lymphocytes. Genotyping was possible from 100 pg of DNA by this method. The estimated allele frequency in 318 Japanese subjects was 0.561 for IgA2*1 and 0.439 for IgA2*2. Analysis of 29 cases of paternity tests suggested that the data follow Mendel's law of inheritance. This genotype could also be detected in whole blood, blood stains, saliva stains, and various organs and tissues. These results suggest the usefulness of the present method for paternity testing and individual identification in forensic medicine.</p>
Keywords polymorphism deoxryibonucleic acid(DNA) immunoglobulin alpha 2 polymerase chain reaction(PCR) allele-specific amplificartion
Amo Type Article
Published Date 1996-02
Publication Title Acta Medica Okayama
Volume volume50
Issue issue1
Publisher Okayama University Medical School
Start Page 1
End Page 9
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8701775
Web of Science KeyUT A1996TY06000001
JaLCDOI 10.18926/AMO/31301
FullText URL fulltext.pdf
Author Yano, Akemi| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood. It was also possible with whole blood left at room temperature for a month and also with the bloodstains left at room temperature for three years. In the heated blood samples, both alleles, Hp2 and Hp1s, were detected in those heated at 100 degrees C for 2 h. In bloodstains, Hp2 and Hp1s were detected in samples heated at 100 degrees C for 2 h and 120 degrees C for 30 min. In addition, the genotype could be detected in samples other than blood such as saliva, hair roots, tissue sections and dental pulps. The present method for Hp genotyping is expected to become a useful method in forensic analysis.</p>
Keywords DNA polymorphism haptoglobin polymerase chain reaction allele-specific amplification personal identification
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 173
End Page 181
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781267
Web of Science KeyUT 000075623600001
JaLCDOI 10.18926/AMO/31305
FullText URL fulltext.pdf
Author Inoue, Seiichi| Yamamoto, Yuji| Okamoto, Osamu| Murakami, Hiroki| Miyaishi, Satoru| Isizu, Hideo|
Abstract <p>A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The products of the second round PCRs were digested with restriction endonucleases for the typing of HLA-DRB1 alleles. By this method, HLA-DRB1 typing was possible from 10 pg of genomic DNA extracted from lymphocytes and from 0.5 microliter of 1,000 times diluted blood without DNA extraction. HLA-DRB1 alleles could be typed from a 2-mm long bloodstained cotton thread prepared from 10 times diluted blood and from a 2-mm thread of whole blood bloodstains stored at room temperature for 2 years. From the mixture of blood of two individuals with different genotypes, DRB1 alleles of the minor component were detected down to 1/1,000 of the major component. This semi-nested PCR-RFLP method is useful for HLA-DRB1 typing from extremely small amounts of DNA and from mixed samples.</p>
Keywords polymorphism HLA-DRB1 polymerase chain reaction dsmi-nested PCR restricton fragment length polymotphism
Amo Type Article
Published Date 1998-12
Publication Title Acta Medica Okayama
Volume volume52
Issue issue6
Publisher Okayama University Medical School
Start Page 289
End Page 296
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9876765
Web of Science KeyUT 000077707300002
JaLCDOI 10.18926/AMO/31707
FullText URL fulltext.pdf
Author Shigeta, Yoshiaki| Yamamoto, Yuji| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evaluated using various forensic samples. The typing results from 70 DNA samples using the new primer pair and the original primer pair were completely identical. One hundred twenty-five amplified fragments from D12S391 alleles were sized correctly within +/- 0.25 bp of the D12S391 allelic ladder. A rare allele, 19.3, previously found only in Caucasians, was found for the first time in a Japanese subject, and it was clearly distinguished from allele 20 by the CE analysis. This detection system was sensitive and could detect D12S391 types from 16 pg of genomic DNA, and from a minor component at a ratio of 1:10 in mixed samples. This system was more useful for the analysis of degraded DNA than was the method using the original primer pair, and could detect D12S391 types from bloodstains that had been stored for 26 years. In addition, the specificity of the method was demonstrated using nonhuman DNA.</p>
Keywords short tandem repeats D12S391 forensic application capillary electrophoresis
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 229
End Page 236
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530506
Web of Science KeyUT 000178668100003
JaLCDOI 10.18926/AMO/31726
FullText URL fulltext.pdf
Author Yoshitome, Kei| Ishikawa, Takaki| Inagaki, Sachiyo| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.</p>
Keywords asphyxia suffocation helium advertising balloon atmosphere lacking in oxygen
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 55
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873946
Web of Science KeyUT 000174031300010
JaLCDOI 10.18926/AMO/31815
FullText URL fulltext.pdf
Author Kamizato, Eigo| Yoshitome, Kei| Yamamoto, Yuji| Iwase, Toshihide| Tsuda, Toshihide| Miyaishi, Satoru| Doi, Hiroyuki|
Abstract <p>The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (&#60;-24, 2544, 4564, and &#62;-65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.</p>
Keywords suicide methods gender-specific legal medicine cluster suicide
Amo Type Original Article
Published Date 2009-08
Publication Title Acta Medica Okayama
Volume volume63
Issue issue4
Publisher Okayama University Medical School
Start Page 177
End Page 186
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19727202
Web of Science KeyUT 000269228400003