このエントリーをはてなブックマークに追加
ID 30512
JaLCDOI
FullText URL
Author
Ishizu, Hideo
Abstract

A method of genotyping IgA2 alleles in the human immunoglobulin alpha 2 heavy chain constant region (C alpha 2 gene) was developed by using the polymerase chain reaction (PCR). By this method, the genotype was determined by discriminating base substitution in the 3'-flanking region of alleles, A2m*1 and A2m*2, which manifest A2m serum types, by nested PCR using allele-specific primers. Three types, IgA2*1/IgA2*1, IgA2*2/IgA2*1, and IgA2*2/IgA2*2, were detected from DNA extracted from lymphocytes. Genotyping was possible from 100 pg of DNA by this method. The estimated allele frequency in 318 Japanese subjects was 0.561 for IgA2*1 and 0.439 for IgA2*2. Analysis of 29 cases of paternity tests suggested that the data follow Mendel's law of inheritance. This genotype could also be detected in whole blood, blood stains, saliva stains, and various organs and tissues. These results suggest the usefulness of the present method for paternity testing and individual identification in forensic medicine.

Keywords
polymorphism
deoxryibonucleic acid(DNA)
immunoglobulin alpha 2
polymerase chain reaction(PCR)
allele-specific amplificartion
Amo Type
Article
Published Date
1996-02
Publication Title
Acta Medica Okayama
Volume
volume50
Issue
issue1
Publisher
Okayama University Medical School
Start Page
1
End Page
9
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT