result 20679 件
| JaLCDOI | 10.18926/11354 |
|---|---|
| Title Alternative | The Problems with "jiritsu-katsudo" in special education for children with physical handicap |
| FullText URL | 001_031_037.pdf |
| Author | Sato, Satoru| |
| Abstract | 本稿では、自立活動をめぐる諸問題について、肢体不自由児教育における問題を中心に整理・検討した。はじめに、自立活動の母体である養護・訓練と関連づけながら、自立活動の目標及び内容の捉え方について論じた。次に、自立活動の指導場面で、どのように指導内容を選定し、指導課題を設定したらよいかについて論じた。さらに、自立活動での指導が、日常的な教育活動と密接な関連をもつようにするための条件を、「経験の再編」（佐伯、1997）といった側面から検討した。最後に、自立活動が個人の能力を向上させることに留まらず、子どもと周囲の人との関係性の向上を考慮した取り組みとして展開される必要があることが指摘された。 |
| Keywords | 自立活動 (jiritsu-katsudo) 特殊教育 (special education) 肢体不自由児 (children with physical handicap) 関連性 (relationship) 経験の再編 (reorganization of experience) |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2001 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 31 |
| End Page | 37 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313656 |
| JaLCDOI | 10.18926/11353 |
|---|---|
| Title Alternative | The Analysis of Questionnaires on Familiar Living Things: in the "Study of Science Instruction A" Class in the 4th Quarter of 2003 Academic Year. |
| FullText URL | 004_011_024.pdf |
| Author | Tanaka, Kenji| Tamaki, Ai| |
| Abstract | 平成15年度第4四半期「理科授業研究A」受講生を回答者として、「多様な生物」に関する意識と現行の小中学校理科（生活科を含む）教科書に示されている「身近な生物」の身近さの程度とを問うアンケート調査を実施し、回答結果を分析・考察した。小学校教員になろうとする学生として、例えば、教科書で示されている生物の1/3は身近な生物ではなく、1/5は聴いたことさえない生物であること、また、「生物多様性条約」「生物多様性国家戦略」を誰一人として聴いたことがないことからわかるように、環境教育に関わる新しい課題「生物の多様性」に対処していくために必要な知識や関心があるとは言い難かった。教科書で示されている生物に対する検討だけでなく、教員養成に関しても検討が求められるものである。 |
| Keywords | 調査報告 (student surveys) 身近な生物 (familiar living things) 理科教科書 (science textbooks) 小学校教員免許状取得希望者 (students in the pre-service teacher education program) |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2004 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 11 |
| End Page | 24 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313625 |
| JaLCDOI | 10.18926/11352 |
|---|---|
| Title Alternative | About the lifelong education at the university : Through the practice of "woodwork seminar" |
| FullText URL | 001_021_030.pdf |
| Author | Yamamoto, Kazufumi| |
| Abstract | 生涯教育の体制は発展途上と言って良いが、その中のものづくり教育について考察した。特に木工分野は学習の機会が少なく、内容的にも問題を感じている。今年、実践した社会人木工セミナーを例に学習支援の必要性と、それを前提とした題材、また同時に結果だけを求める学習者の意識的な問題が明らかになった。この講座の設定方法を巡って問題を感じたため、大学における社会人教育の窓口について考察を行った。文部省生涯学習審議会はその拡充を求めており、特に公開講座を授業科目で行うことは有効である。これに半歩踏み出したかたちで木工講座の具体案を提案した。 |
| Keywords | 社会人教育 生涯教育 (lifelong education) 木工講座 (woodwork lecture) 公開講座 (open class) |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2001 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 21 |
| End Page | 30 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313707 |
| JaLCDOI | 10.18926/11351 |
|---|---|
| Title Alternative | An Improvement of the Social Studies Curriculum of the Third and Fourth Grades of Elementary Schools |
| FullText URL | 004_001_010.pdf |
| Author | Kuwabara, Toshinori| |
| Abstract | 本研究は、現行の小学校学習指導要領社会科の内容編成を分析し、その問題点を明らかにするとともに、学習指導要領にとらわれることなく、市民的資質育成を目指して子供の科学的社会認識を形成する社会科を実践するためには、どのように教育内容を編成すればよいかを明らかにしようとするものである。そのために、本研究では特に中学年カリキュラムに注目する。そして、中学年では教育内容としていかなる科学的な概念や、法則・理論を選抜し配列すべきかという原理とその方法を、アメリカで発行されている小学校社会科教科書の分析を手がかりに明らかにしていく。分析の結果、中学年においては子供たちの生活する共同体に関わる科学的な概念を抽出してカリキュラムを編成する方法が考えられること、ただし、それは単純な環境拡大原理によるものではないことが明らかになった。 |
| Keywords | 小学校社会科 (Elementary School Social Studies) アメリカ社会科 (Social Studies in the United States) 市民的資質育成 (Building Citizenship) カリキュラム |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2004 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 10 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313537 |
| JaLCDOI | 10.18926/11350 |
|---|---|
| Title Alternative | A Teaching Research of the Science related Careers in the Elementary School Science, based by the American Textbooks Discover the Wonder |
| FullText URL | 001_011_020.pdf |
| Author | Tanaka, Kenji| |
| Abstract | 本（平成12）年度の講義「初等理科教育学A（理科授業研究）」では、アメリカの初等理科教科書「Discover the Wonder」に基づき、授業研究の試みを行わせるとともにこの教科書で扱われている People at Work/Ex-ploring Careers を参考にして、課題［科学にかかわる職業・仕事についてのガイダンス］などによって初等教育段階における科学にかかわる職業（仕事）のガイダンスの取り扱いを検討させてきた。本稿は、この実践報告である。様々な特徴や工夫を見出し、教科「理科」や「総合的な学習の時間」における科学にかかわる職業のガイダンスの授業実践などに向けて、指導案を作ることができ、さらに受講結果についての総括的な自己評価や講義全体についての評価からも、ほぼ所定の成果を得たと考えている。 |
| Keywords | 初等教育段階 職業 ガイダンス アメリカ 教科書 |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2001 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 11 |
| End Page | 20 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313539 |
| JaLCDOI | 10.18926/11349 |
|---|---|
| Title Alternative | A Study of developing sense and thinking in the Social Studies Class: On the Basic of Contrasting Some Theories about Value Judgment Teaching |
| FullText URL | 001_001_009.pdf |
| Author | Kuwabara, Toshinori| |
| Abstract | 本研究では、社会科で育成すべき見方・考え方のうち社会事象に対する価値判断に焦点をあて、価値的判断力を育成する授業構成にどのような方法があるかを社会科教育学研究の成果に基づいて考察した。まず、現在わが国の社会科教育学研究の成果として提案されている判断力育成教育原理を検討した。それらは、社会的決定を基盤とするものと、個人的決定を基盤とするものに区分され、分析の結果、前者は社会化を目指し、後者は個性化を目指していることが明らかとなった。さらに、価値的知識の解釈過程を子どもの思考に組み込んだ授業構成を提案し、価値判断力の育成を目指す社会科授業構成の前提を示した。 |
| Keywords | 判断力育成 (Developing Judgment) 価値認識 (Value Cognition) 価値的知識 (Value Knowledge) 法的判断 (Law Judgment) |
| Publication Title | 岡山大学教育実践総合センター紀要 |
| Published Date | 2001 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 9 |
| ISSN | 1346-3705 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313872 |
| Title Alternative | Mutation of Fungicide Tolerance in Fusarium spp. |
|---|---|
| FullText URL | 005_001_047_060.pdf |
| Author | Wu, Jiri| Takeda, Kazuyoshi| |
| Abstract | Strains of Fusarium, causal fungi of scab disease of cereal crops, were examined for the mutation of topsin tolerance. The mutation rate of topsin tolerant spores was 10-5~10-6 and was not affected by the irradiation of ultraviolet rays nor application of topsin to the culture medium. Highly tolerant strains were easily selected on the screening medium containing topsin. Finally topsin was not effective to the selected tolerant mutants. The topsin tolerance was parallel to "benrate" tolerance. The tolerance was transmitted through hyphae, conidium and ascospore. The growth rate of hyphae of the tolerant mutants was lower than that of the original strains, but the conidia formation and the virulence of the mutants were comparable to the original strains. Since the heavy application of fungicides may increase the fungicide-tolerant mutants, crop varieties resistant to scab disease must be developed. |
| Keywords | Fusarium spp. Fungicide tolerance Mutation. |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 47 |
| End Page | 60 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Phenol Reaction of Kernels and Chromosome Location of Phenol Reaction Genes in the Genus Triticum |
|---|---|
| FullText URL | 005_001_061_068.pdf |
| Author | Chang, Cheng Lin| Takeda, Shin| Takeda, Kazuyoshi| |
| Abstract | A total of 3,606 accessions of the genus Triticum involving diploid, tetraploid, hexaploid, and synthetic wheat and 7 Aegilops materials were tested for the phenol reaction in kernels. All hexaploid wheat showed a positive reaction to phenol, but deffered in staining degree. One of the synthetic wheat lines showed a negative reaction to phenol. Using monosomics, ditelosomics and nulli-tetrasomics in the common wheat cv Chinese Spring (Triticum aestivum L.), and Langdon (Triticum turgidum var. durum) disomic substitutions, genes controlling phenol reaction of kernels were located on chromosomes 2A, 2B and 2D. Synteny of the chromosome region involving the phenol reaction gene in some gramineous plants was discussed. |
| Keywords | Phenol reaction Triticum Chromosome |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 61 |
| End Page | 68 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | ラン科植物に発生するシンビジウムモザイクウイルスの血清学的検出 |
|---|---|
| FullText URL | 005__001_039_046.pdf |
| Author | I Wayan, Gara| Kondo, Hideki| Maeda, Takanori| |
| Abstract | Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were examind for their usefulness in the detection of cymbidium mosaic virus (CyMV) in orchids. The minimum detection levels of CyMV by these methods were 100 ng/ml in purified preparations and at 10-4 dilution of extracts from infected leaves of orchids could be detected by these methods. Although DIA took 5 to 6 hours for the detection of the virus, it was reliable method for diagnosis of a large-number of samples. On the other hand, RIPA, which enabled detection of CyMV within a few minutes with sensitivity similar to that of DIA, will be suitable as a rapid and handy tool for virus disease diagnosis in orchids. Moreover, by RIPA, we could detect CyMV and odontoglossum ringspot virus (ORSV) simultaneously form doubly infected plant. |
| Keywords | Serological detection Cymbidium mosaic virus Orchid |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 39 |
| End Page | 46 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313874 |
| Title Alternative | QTL Mapping for Powdery Mildew (Erysiphe graminis DC. f. sp. hordei EM Marchal) Resistance in Barley (Hordeum vulgare L.) |
|---|---|
| FullText URL | 005_001_069_078.pdf |
| Author | Iwasa, Tomohiko| Heta, Hideo| Takeda, Kazuyoshi| |
| Abstract | Powdery mildew, caused by Erysiphe graminis DC. f. sp. hordei EM Marchal, is a serious disease of Barley (Hordeum vulgare L.). In this study, we used molecular markers to identify the chromosomal locations carrying genes for powdery mildew resistance and to estimate the effect of each gene. Doubled haploid lines derived from Steptoe×Morex (S/M), Harrington×TR306 (H/T) and their parental were inoculated with five powdery mildew strains. Several quantitative trait loci (QTL) controlling E. graminis resistance were found and lacated on chromosome 4H, 5H, 6H and 7H in S/M. On the other hand, no QTL was detected in H/T but Harrington had a major resistant gene (Mlg) for powdery mildew resistance. Maker-assisted selection was conducted to examine the effect of accumulation for mildew resistance. There was a significant interaction between QTLs lacated in 4H and 7H, which suggested the presence of an epistatic effect between these QTLs. |
| Keywords | Hordeum vulgare Erysiphe graminis QTL mapping DH lines |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 69 |
| End Page | 78 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 日本におけるCymbidium属植物から分離されて生物学的性質の異なるOdontoglossum Ringspot Virus分離株のペプチドマッピングによる比較 |
|---|---|
| FullText URL | 005_001_031_038.pdf |
| Author | Kondo, Hideki| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Symptoms on Cymbidium, double-stranded (ds) RNA pattern and peptide mapping of coat protein (CP) of five isolates of odontoglossum ringspot virus from Cymbidium in Japan were compared. One of the isolates, Cy-1, that produced unique symptoms on Cymbidium, showed a distinct peptide mapping pattern from those of the other four isolates by partial digestion of CP with pepsin. All the isolates produced three major dsRNA species of Mr=4.3,1.4 and 0.6×106 in the infected plants. |
| Keywords | Odontoglossum ringspot virus Cymbidium Peptide mapping Double-stranded RNA |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 31 |
| End Page | 38 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313419 |
| Title Alternative | 2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出 |
|---|---|
| FullText URL | 005_001_023_030.pdf |
| Author | Maeda, Takanori| Sako, Nobumichi| Inouye, Narinobu| |
| Abstract | To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection. |
| Keywords | Cucumber mosaic virus Monoclonal antibody Simplified ELISA |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 23 |
| End Page | 30 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出 |
|---|---|
| FullText URL | 005_001_079_096.pdf |
| Author | Saito, Minako| Kiguchi, Tadahiko| Tamada, Tetsuo| |
| Abstract | Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV. |
| Keywords | Sugar beet Rhizomania BNYVV RNA detection Nonradioactive cDNA probe |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 79 |
| End Page | 96 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Measurements of Response of Barley and Wheat to Enviromental Factors with an Open System Porometer |
|---|---|
| FullText URL | 005_001_011_021.pdf |
| Author | Maitani, Toshihiko| Kashiwagi, Yoshiaki| |
| Abstract | The rates of photosynthesis and transpiration were measured in barley and wheat under various environmental conditions, with an open system porometer. The rates of photosynthesis and transpiration in the horizontal leaf and vertical leaf had different diurnal variations. The rate of photosynthesis in the vertical leaf was highest in the morning and in the afternoon, while that in the horizontal leaf was highest before noon. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v SARI and Akanmugi) of barley grown in submerged soil conditions. At the end of April, chlorophyll contents had decreased and the maintenance respiration acquired in spite of continuous transpiration. The rapid change of photosynthetically active radiation did not affect the rates of photosynthesis or stomatal conductance of SARI grown in submerged soil. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v. Hongmangmai and Haruhikari) of wheat grown under different soil water conditions. Chlorophyll content tended to increase in dry soil conditions. Hongmangmai had a higher chlorophyll content than Haruhikari, even at the beginning of May. Hongmangmai had large photosynthetic rate and small transpiration rates under dry soil conditions. These confirm that Hongmangmai has a prominent drought stress tolerance. The open system porometer and the chlorophyll meter may be very useful for comparing physiological characteristics of the plant's response to environmental factors and clarifying differences between plant species. |
| Keywords | Barley Hongmangmai Photosynthesis rate Chlorophyll content Submerged soil Dry soil |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 11 |
| End Page | 21 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング |
|---|---|
| FullText URL | 006_001_053_064.pdf |
| Author | Murata, Minoru| |
| Abstract | Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (Ⅰ-Ⅵ) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class Ⅰ, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes. |
| Keywords | Cytoplasm substitution line Differential screening Midget chromosome Rye Wheat |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 53 |
| End Page | 64 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | ホウレンソウ種子に存在するα-グルコシダーゼの分子多型変化 |
|---|---|
| FullText URL | 005_001_001_009.pdf |
| Author | Furui, Satoshi| Sugimoto, Manabu| Suzuki, Yukio| |
| Abstract | Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70 kDa by gel filtration, respectively. α-Glucosidase A had high activity not only toward maltooligosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 65℃ for 20 min. On the other hand, α-glucosidase B had high activity toward maltooligosaccharides but faint activity toward α-glucans. The optimum pH was 5.0 and no activity was found after incubation at 65℃ for 20 min. The enzymatic and immunological properties of α-glucosidase A and B were similar to those of α-glucosidase. Ⅰor Ⅱ, and α-glucosidase Ⅲ or Ⅳ, isolated from spinach seeds without 4℃ storage, respectively. These findings suggest that the α-glucosidase in spinach seeds is modified to be two molecular forms. |
| Keywords | Spinach α-Glucosidase Multiple molecular forms |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 9 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | エビネから分離されたインゲンマメ黄斑モザイクウイルスの諸性質 |
|---|---|
| FullText URL | 006_001_043_051.pdf |
| Author | Matsumoto, Jun-Ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Bean yellow mosaic virus (BYMV) was isolated from Calanthe sp.showing mild chlorosis on the leaves, collected in Yamaguchi Prefecture in Japan. The virus was transmitted by the aphid Myzus persicae in a non-persistent manner, and by sap-inoculation to 29 out of 46 plant species from 9 out of 12 families tested. Stability in crude sap, morphology of virus particles, shape of cylidrical inclusions and the presence of cytoplasmic crystalline inclusions in the infected cells were similar to those of BYMV isolates previously reported. The virus contained a single protein species with a molecular weight of 35,000. In a microprecipition test and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the virus showed a close serological relationship to isolates of BYMV from both crocus and gladiolus, and showed a distant relationship to clover yellow vein virus. Three BYMV isolates used in this study were found to be serologically related to each other, but the virus was more closely related to the BYMV crocus isolate than to gladiolus isolate. |
| Keywords | Calanthe sp. bean yellow mosaic virus clover yellow vein virus serological relationship |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 51 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Varietal Variation and Mechanism of Hull-cracked Grains in Two-rowed Barley |
|---|---|
| FullText URL | 004_001_089_096.pdf |
| Author | Kanatani, Ryoichi| Takeda, Kazuyoshi| |
| Abstract | Hull-cracked grain which causes low germinability and low malt extract has been observed in malting barley varieties mainly in the western part of Japan. In the first part of this study, more than 600 two-rowed barley varieties were examined for the percentage of hull-cracked grains. A markedly skewed frequency distribution pattern was observed for varietal variation of hull-cracked grain percentage. More than 80% of the varieties developed less than 5% hull-cracked grains, while a few of the varieties frequently developed the hull-cracked grains. The maximum hull-cracked grain percentage was as high as 61% in Yoshikei 16. Improved varieties developed hull-cracked grains more frequently than the local varieties, indicating varietal improvement indirectly caused the hull-cracked grains. In the second part, nine two-rowed varieties were grown in eight different conditions to analyze the mechanism of hull-cracking. Variances due to varieties, environment and their interaction were all statistically significant. Some of the varieties developed almost no hull-cracked grains throughout the environmental conditions examined, while others sharply responded to the environmental conditions. The environmental correlation coefficient between 1,000-kernel weight and hull-cracked grains was as high as 0.918, indicating that hull-cracked grains had developed under favorable ripening conditions. |
| Keywords | Barley Varietal varietal Hull-crached grain Ripening |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 89 |
| End Page | 96 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | The Characteristics of Trace Metal Distribution of Artificial and Natural-Origin Suspended Particulate Matter (SPM) by grain size |
|---|---|
| FullText URL | 006_001_029_041.pdf |
| Author | Muramoto, Shigeki| Maitani, Toshihiko| Hiraoka, Naoko| Aoyama, Isao| |
| Abstract | Air pollution caused by suspended particulate matter (SPM) has increased remarkedly in industrial areas, and this has become an emergency issue in the global environment. Atomospheric SPM influences the local ecosystem including various plants and animals. It is necessary to examine the distribution characteristics of such trace metals in fine particulates (especially those with a diameter (φ) of less than 2.1μm). The Environment Agency of the Japanese goverment will complete analysis of the a concentration of suspended particulate matter less than 2.4μm by the end of 1999, and will be examining the relationship between the concentration of suspended particulate matter (0.1-9.0μm) with consideration given to the particle size throughout two years. It became clear that the metals of artificial origin such as Pb, V, Cd, Ni, Cr, existed in fine particulates (0.1 or more 2.0μm). By contrast, the metals of natural origin, such as Ti, Mn, Mg, Sr, mainly existed in coarse Area in Japan. |
| Keywords | Atomospheric Suspended particulate matter (SPM) Natural-origin metal Artificial-origin metal Biological effects |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 29 |
| End Page | 41 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | QTL Mapping for Water Sensitivity in Barley Seeds |
|---|---|
| FullText URL | 006_001_021_028.pdf |
| Author | Iwasa, Tomohiko| Takahashi, Hidekazu| Takeda, Kazuyoshi| |
| Abstract | Sensitivity to water condition during germination is an important character in malting barley because it affects malt poduction. Water sensitivity in malting barley is defined as the defference in germination percentage between the seeds in standard and excess-water conditions. Usually standard and excess-water condition means 4 and 8 ml water in a 90 mm perti dish, respectively. Takeda and Fukuyama (1983) examined more than 4,000 barley accessions and found 0～100% water sensitivity. This means complete to no suppression of germination by an excess-water condition. In this study, quantiative trait loci (QTL) analysis was applied to analize the inheritance of water sensitivity. Two sets of doubled haploid (DH) populations derived from Steptoe×Morex (S/M) and Harrington×TR306 (H/T) crosses,were grown in the experiment field of the Research Institute for Bioresources, Okayama University. Bulbosum method was adapted to develop DH lines (Chen and Hayes 1989). Water sensitivities of 150 (S/M) and (H/T) lines and their parents were evaluated using 50 seeds each and the germination test (25℃, 4 days) was repeated four times. Water sensitivity showed a large variation (Fig.1) and the heritability of the trait was 0.78 and 0.72 in S/M and H/T, respectively, indicating that about three-quarters of the phenotypic variance was genetic in origin. Three (S/M) and one (H/T) significant QTL were detected and 23% (S/M) and 25% (H/T) of the phenotypic variance was explained by the QTL. These QTL were located on chromosomes 2H,3H,6H and 7H (Table 1, Fig. 2). QTL relating to germination traits were lacated near the QTL controlling water sensitivity (Fig. 3). Interaction among the QTL was small (Table 2). Marker-assisted selection was effective (Table 3). |
| Keywords | barley water sensitivity QTL analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 21 |
| End Page | 28 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
