result 20679 件
| Title Alternative | Some Properties of Azuki Bean Mosaic Virus Isolated from Soybean Plants in Ibaraki Prefecture |
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| FullText URL | 004_002_215_227.pdf |
| Author | Takahashi, Kokichi| Inouye, Narinobu| Maeda, Takanori| Mitsuhata, Koji| Chida, Shigeki| Takahashi, Yoshiyuki| |
| Abstract | Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40～50% less than that of healthy plants. |
| Keywords | Potyvirus Azuki bean mosaic virus Glycine max Properties |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 215 |
| End Page | 227 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 大麦網斑病抵抗性の選抜効果 |
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| FullText URL | 003_001_043_053.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. |
| Keywords | Net blotch Pyrenophora teres Selection Barley Disease resistance |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 53 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120004537480 |
| Title Alternative | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
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| FullText URL | 003_001_035_041.pdf |
| Author | Hou, Fwu-Fenn| Thseng, Fu-Sheng| Wu, Shu-Tu| Takeda, Kazuyoshi| |
| Abstract | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| Keywords | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 35 |
| End Page | 41 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Ixia から分離された bean yellow mosaic virus |
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| FullText URL | 004_002_201_213.pdf |
| Author | Tsuji, Toshiya| Maeda, Takanori| Kondo, Hideki| Inouye, Narinobu| |
| Abstract | A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. |
| Keywords | Ixia hybrida Bean yellow mosaic virus Potyvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 201 |
| End Page | 213 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313395 |
| Title Alternative | Sorghum Resistance to the Sugarcane Aphid, Melanaphis sacchari (Zehntenr) Amounts of Surface Wax and Nutritional Components |
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| FullText URL | 003_001_027_034.pdf |
| Author | Tsumuki, Hisaaki| Kanehisa, Katsuo| Moharramipour, Saeid| |
| Abstract | The differences in the amounts of leaf surface wax and nutritional components such as sugar and free amino acids were compared among resistant (PE 954177), moderately resistant (IS 84) and susceptible (Redlan B) sorghum varieties to the sugarcane aphid. Melanaphis sacchari (Zehntner). (1) The kind of aphid appearing on the leaves of the sorghum varieties was only the sugarcane aphid, Melanaphis sacchari in the field. The number of aphids infesting a leaf was ca 0,30 and 3,500 in the resistant, moderately resistant and susceptible varieties at the middle of August, respectively. (2) The amount of leaf surface wax was approximately constant among these three varieties. (3) The total sugar content was slightly higher in the two resistant varieties than in the susceptible variety. (4) The total free amino acid concentration was higher in the two resistant varieties than in the susceptible variety. (5) These findings suggest that neither leaf surface wax nor nutritional components seem to affect the aphid populations on the leaves of the three sorghum varieties, showing that biogenic substances such as dhurrin, benzylalcohol, p-hydroxybenzaldehyde, aconitic acide, etc. may affect the aphid populations. |
| Keywords | Sorghum Sugarcane aphid Melanaphis sacchari Leaf surface wax Amino acid |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 27 |
| End Page | 34 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Some Properties of Cymbidium Mosaic Virus Isolated from Calanthe spp. |
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| FullText URL | 004_002_187_199.pdf |
| Author | Matsumoto, Jun-ichi| Urabe, Shinji| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| Inouye, Narinobu| |
| Abstract | Cymbidium mosaic virus(CyMV) was isolated from Calanthe spp. showing mosaic on the leaves, collected in Yamaguchi and Kyoto Prefectures in 1986～1993. CyMV, Cal. 90-1 isolate was transmitted by sapinoculation to 12 out of 37 species in 7 out of 9 families. Sap from diseaded Tetragonia expansa was infective to Chenopodium amaranticolor after dilution to 10-5 but not 10-6, after heating at 65℃ for 10 min but not 70℃, and after 1 month at 20℃ but not 2 months. The virus particles were flexuous rod, about 475 nm long. The virus was purified from diseased T. expansa leaves and contained a single protein species of Mr27,800. The Mr of the capsid proteins(Cal. 90-1) was similar to those of two ohter CyMV isolates(Cal. 90-4, Cal. 93-14).Cal. 90-1 and Cal. 93-14 reacted with antiserum to the Cymbidium isolate (Cy-16), suggesting that Cal. 90-1 was serologically very similar to the other two CyMV isolates. Two species of dsRNA were isolated from plants infected with Cal-1 and they were similar to those of two other CyMv isolates. |
| Keywords | Calanthe spp. Cymbidium mosaic virus Potexvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 187 |
| End Page | 199 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313956 |
| Title Alternative | Quantitative Variations of a Resistance Substance, DIMBOA, against Aphids in Wheat Varieties. |
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| FullText URL | 003_001_017_026.pdf |
| Author | Kanehisa, Katsuo| Awan Rustamani, Maqsood| Cheng, Wen-Yi| Tsumuki, Hisaaki| Shiraga, Takashi| |
| Abstract | Aphids sometimes severely infest wheat plants, mainly sucking phloem sap and disrupting tissues, and in a few cases act as virus vectors. There are resistant and susceptible varieties of wheat against aphids. DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) is a substance causing resistance to animals including aphids. The change in the amounts of DIMBOA with growth in eight wheat varieties was estimated in 1990 and 1991. Wheat seeds were sown at mid-November in the former year and harvested in mid-June. Aphids appeared from early April, increased with the growth of the wheat, and finally decreased with the senescence of the wheat. Rhopalosiphum maidis appeared early in the season, R. padi appeared late, Schizaphis graminum and Sitovion akebiae appeared intermittently in the season. S. graminum appeared more frequently on wheat than barley. DIMBOA was detected from wheat but not from barley. Gramine (N,N-dimethyl-indole-3-methanamine) was detected from barley, and is known as an important resistance substance. However gramine could not be detected in wheat. DIMBOA was found in higher amounts in young wheat, and gradually decreased with growth. A clear relationship between the aphid population and DIMBOA amounts could not be observed. However, all the wheat varieties used in this experiment seemed to have resistance against aphids. The resistance was compared with barley susceptible lines. DIMBOA was presumed to share the property of resistance with aconitic acid in wheat. |
| Keywords | DIMBOA content Wheat Aphid Resistant varaity Susceptible variety |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 17 |
| End Page | 26 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
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| FullText URL | 004_002_164_174.pdf |
| Author | Gara, I Wayan| Kondo, Hideki| Maeda, Takanori| Mitsuhata, Koji| Inouye, Narinobu| |
| Abstract | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| Keywords | Vanda orchid Cymbidium mosaic virus Potexvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 164 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313836 |
| Title Alternative | Reaction of the Resistant Gene ml-o to Various Barley Powdery Mildew Strains |
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| FullText URL | 003_001_011_016.pdf |
| Author | Heta, Hideo| Takeda, Kazuyoshi| |
| Abstract | Eight powdery mildew strains, four collected from the field and the other four derived from a cross between two strains, were inoculated to seven mutant barley lines having the ml-o gene for powdery mildew resistance and their original varieties of barley. These eight powdery mildew strains showed a distinctive reaction to the nine testers having various resistant genes. Onthe other hand, the original barley varieties showed a similar reaction to all the powdery mildew strains inoculated, indicating that the genetic background of these varieties was similar. In comparison with the ml-o mutants and their original varieties, the type of lesion did not change in most cases, but the number of lesions decreased significantly without exception. In conclusion, the powdery mildew resistant gene ml-o does not affect the type of lesions but it reduces the number of lesions. |
| Keywords | Powdery mildew resistance Barley ml-o gene Pathogenicity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 11 |
| End Page | 16 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Studies on Host Selection, Development and Reproduction of Melanaphis sacchari (Zehntner) |
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| FullText URL | 003_001_005_010.pdf |
| Author | Kawada, Kazuo| |
| Abstract | The host selection, development and reproduction of sugarcane aphid, Melanaphis sacchari (Zehntner) were studied. Both alate and apterous virginoparous adults showed a stronger tendency of preference to a susceptible strain of sorghum and Cuba grass, Sorghum helepense (Persoon) than a resistant strain of sorghum and Eulalia, Miscanthus sinensis (Anderss). The number of days for larval development was increased with a decrease in longevity and fecundity in aphids reared on resistant sorghum at 25℃ under a 16-hour photoperiodic condition. |
| Keywords | Sorghum Host selection Sugarcane aphids |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 5 |
| End Page | 10 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Detection of the Viruses Occurring in Oriental Cymbidium in Japan |
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| FullText URL | 004_002_149_162.pdf |
| Author | Kondo, Hideki| Maeda, Takanori| Mitsuhata, Koji| Inouye, Narinobu| |
| Abstract | A survey of virus diseases occurring in Oriental Cymbidium collected from a commerical nursery and home garden in Japan was conducted in 1991-1994. Identification of the vurus was based on partcle morphology, symptomatology in indicator plants, ultrastructure of infected cells and serology. Four viruses, odontoglossum ringspot tabamovirus(ORSV), cymbidium mosaic potexvirus(CyMV), orchid fleck virus (ORV) and a previously underscribed spherical virus, were found in 27 out of 37 Cymbidium plants tested. ORSV was detected from 11 plants belinging to Cym. ensifolium, Cym. forrestii, Cym. goeringii, Cym. kanran, Cym. sinense and Cymbidium spp. showing chlorotic streaks and/or mild mosaic. CyMV was isolated from only one plant of Cymbidium sp. showing mosaic and necrotic spots on leaves. In negatibvely stained dip preparations from plants infected with ORSV and CyMV, rod shaped particles of ca. 310 nm and flexuous rod-shaped ca. 475 nm in length were observed, respectively. The viruses were reacted strongly with respective antiserum to each virus in immunosorbent electron microcopy and inderect ELISA. OFV was isolated from four plants of Cym. formosanum, Cym. kanran, Cym. sinense and Cymbidium sp. showing mosaic and necrotic flecks. The virus had non-enveloped, bullet-shaped particles about 40×120～150 nm in dip preparation. The undescribed spherical virus, ca. 28 nm diameter, was isolated from 11 plants of Cym. forrestii, Cym. goeringii and Cymbidium spp. showing stunting and chlorotic streaks on newly developed leaves. The virus was mechanically transmitted only to Cymbidium orchids. Previously, we designated it as cymbidium chlorotic mosaic sobemovirus(CyCMV)(Kondo et al,1994),as the virus was considered to be a new member of the genus Sobemovirsu. |
| Keywords | Oriental Cymbidium Odontoglossum ringspot tobamovirus Cymbidium mosaic potexvirus Orchid fleck virus Cymbidium chlorotic mosaic sobemovirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 149 |
| End Page | 162 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313470 |
| Title Alternative | カクヤリグサ科一年生雑草における数量分類学的研究 |
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| FullText URL | 002_001_123_134.pdf |
| Author | Muhamad Ahmad, Chozin| Satou, Kazuhiro| Yasuda, Shozo| |
| Abstract | Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. |
| Keywords | Cyperus iria Cyperus microiria Cyperus amuricus Numerical taxonomy Speciation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 123 |
| End Page | 134 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313639 |
| Title Alternative | オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較 |
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| FullText URL | 004_002_137_147.pdf |
| Author | Ikegami, Masato| Inouye, Narinobu| |
| Abstract | The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%. |
| Keywords | Tobamovirus Odontoglossum ringspot virus Nucleotide sequence Genome organization |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 137 |
| End Page | 147 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Comparison of Resistance to Powdery Mildew between Wild Barley (Hordeum spontaneum C. Koch) |
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| FullText URL | 002_001_111_122.pdf |
| Author | Fukuyama, Toshinori| Heta, Hideo| Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | A total of 162 strains of wild barley, Hordeum spontaneum C. Koch originating from Iran, Iraq, Turkey and Central Asia, were tested for resistance to powdery mildew. Then, the variation of resistance was compared with that of 145 local varieties of cultivated barley (Hordeum vulgare L.) originating from the same region of the wild barley collection. Ten different isolates of the parasite with Japanese origin were separately inoculated onto the first leaves of the host plants. The infection types were classified into the following: i, immunelike; R, highly resistant; M, moderately resistant; and S, highly susceptible. Resistant strains with i, R or M infection type were more frequent among wild barleys as compared with the cultivated forms. It is noteworthy that among these three resistant reactions,the M type was most frequent in the wild barley. To compare the degree of resistance to a total of 10 isolates, the resistance score was calculated in each of the wild and cultivated strains as the following: Scores 1,2,3 and 4 were given to the infection types of i, R, M and S, respectively, and the mean score for 10 isolates was calculated. Wild barley showed significantly low resistance scores as compared with those of cultivated barley. This was also confirmed by the cluster analysis; the cluster with more resistance to 10 isolates consisted of many strains of wild barley. Next, the resistance of wild barley was characterized by their broader effective ranges to different isolates. According to the x2 test for independence of reactions to two different isolates, the resistant factor(s) involved in wild barley was confirmed to be rather non-specific to the parasite. It was concluded that H. spontaneum may be useful genetic resources for the breeding of resistance to powdery mildew as well as local varieties. |
| Keywords | Hordeum spontaneum Barley Powdery mildew Resistance |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 111 |
| End Page | 122 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313607 |
| Title Alternative | Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe |
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| FullText URL | 004_002_119_135.pdf |
| Author | Inouye, Narinobu| Matsumoto, Jun-ichi| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| |
| Abstract | Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV･So from Cymbidium. |
| Keywords | Calanthe Orchid fleck virus Calanthe yellowish fleck disease |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 119 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314037 |
| Title Alternative | 大麦うどんこ病菌(Erysiphe graminis DC. f. sp. hordei)の地理的分化 |
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| FullText URL | 002_001_103_110.pdf |
| Author | Konishi, Takeo| Heta, Hideo| |
| Abstract | Fifteen cultures of barley powdery mildew fungus (Erysiphe graminis DC. f. sp. hordei) isolated in different regions of the world were inoculated to 120 barley varieties. Infection scores varied among the cultures and barley varieties, and their interactions were observed. Principal component analysis of the infection scores revealed that the cultures could be classified into three groups by first and second components (contributing 40.2% and 15.6% of the total variance, respectively), and that two Japanese cultures could be distinguished from the others isolated in Europe and North America. This indicates that the fungus is geographically differentiated in the reaction of the barley varieties to the cultures. Furthermore, East Asian barley varieties differed from European ones in their reactions to the culltures, while barley varieties from regions between East Asia and Europe showed a large genetic diversity in their reactions. |
| Keywords | Barley Powdery mildew Geographic distribution Differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 103 |
| End Page | 110 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | インドネシアのランに発生するウイルスの検索と同定 |
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| FullText URL | 004_002_109_118.pdf |
| Author | Inouye, Narinobu| Gara, I Wayan| |
| Abstract | Three viruses, Cymbidium mosaic virsu(CyMV), odontoglossum ringspot virus (ORSV) and an unidentified potyvirus were found in the orchids in Indonesia. CyMV was detected from orchids in 8 genera, namely Aranthera, Calanthe, Cattleya, Cymbidium, Gromatophyllum, Phalaenopsis,Oncidium and Vanda. The virus was widespread in many orchids in Indonesia and was common in Aranthera and Calanthe, thus being an economically important virus in Indonesia.ORSV was also detected in orchids of 5 genera, namely Bulvophyllum,Calanthe, Cattleya,Oncidium and Phalaenopsis. The unidentified potyvirus was found in Aranthera. |
| Keywords | Cymbidium mosaic virus Odontoglossum ringspot virus Unidentified potyvirus Identification Orchids in Indonesia |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 109 |
| End Page | 118 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
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| FullText URL | 003_002_163_174.pdf |
| Author | Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| Keywords | Odontoglossum ringspot virus Calanthe sp. Identification |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 163 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Sources of Resistance to Net Blotch in Barley Germplasm |
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| FullText URL | 002_001_091_102.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
| Keywords | Barley Net blotch Disease resistance Genetic resources Race differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 91 |
| End Page | 102 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313965 |
| Title Alternative | Diallel Analysis for the Percentage of Grains with Hull Rupture in F2 Populations of Two-rowed Barley |
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| FullText URL | 002_001_079_089.pdf |
| Author | Wu, Jiri| Takeda, Kazuyoshi| Kanatani, Ryoichi| |
| Abstract | Half diallel F2 populations crossed among eight two-rowed barley varieties with warious percentages of hull ruptured grains were raised to analyze the inheritance of the trait. The percentage of grains with hull rupture varied from 0 to 87% among the parents. The diallel analysis revealed the following: the trait was predominantly controlled by the additive genes, and the dominance effect of some parents was also significant. However, the epistatic effect of the genes was not significant. The average dominance was 0.97. The heritability value was estimated as 0.57 and 0.91 in a narrow and a broad sense, respectivelly. The percentage of grains with hull rupture showed continuous and transgressive segregations in 28 F2 populations derived from half diallel crosses among eight parents. Heritability of the trait in a broad sense was 0.43~0.80(0.65 on average) in 28 F2 populations. |
| Keywords | Barley Grain quality Hull rupture Diallel analysis Heritability |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 79 |
| End Page | 89 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
