| Author | 河田 和雄| |
|---|---|
| Published Date | 1971-03 |
| Publication Title | 農学研究 |
| Volume | volume54 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | 河田 和雄| |
|---|---|
| Published Date | 1972-03 |
| Publication Title | 農学研究 |
| Volume | volume54 |
| Issue | issue3 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | Changes of Gramine Contents as an Aphid Resistant Substance with Barley Growth |
|---|---|
| FullText URL | 001_002_105_112.pdf |
| Author | Kanehisa, Katsuo| Rustamani, Maqsood A.| Tsumuki, Hisaaki| Kasahara, Toshihiko| Shiraga, Takashi| |
| Abstract | Barley plants may be severely damaged by aphids,mainly because they may transmit viruses,suck food and disrupt tissues. There are resistant lines and susceptible lines in barley to aphids. The changing amounts of gramine from seeding to mature stages were investigated on 27 lines from 1989 to 1991. Barley was sown aound November 20 in the year and harvested in early June. Higher amount of gramine in seeding stage in all lines were detected,then gradually decreased with barley growth until 2 to 3 weeks before the earing stages. At this time aphids had appeared on the barley leaves and increased in population. The susceptible lines had a tendency of greater decrease in gramine content than the resistant lines at the time of aphid population growth. The maximum aphid population was observed from the end of April to early May. The gramine content was almost constant this time. The degradation mechanisms may be important factors determining the defference of resistant and susceptible lines at the aphid population growth stage. The susceptible lines may have a higher activity of degradation mechanism. |
| Keywords | Gramine content Barley Aphid Resistant lines Susceptible lines |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1993 |
| Volume | volume1 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 112 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Quantitative Variations of a Resistance Substance, DIMBOA, against Aphids in Wheat Varieties. |
|---|---|
| FullText URL | 003_001_017_026.pdf |
| Author | Kanehisa, Katsuo| Awan Rustamani, Maqsood| Cheng, Wen-Yi| Tsumuki, Hisaaki| Shiraga, Takashi| |
| Abstract | Aphids sometimes severely infest wheat plants, mainly sucking phloem sap and disrupting tissues, and in a few cases act as virus vectors. There are resistant and susceptible varieties of wheat against aphids. DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) is a substance causing resistance to animals including aphids. The change in the amounts of DIMBOA with growth in eight wheat varieties was estimated in 1990 and 1991. Wheat seeds were sown at mid-November in the former year and harvested in mid-June. Aphids appeared from early April, increased with the growth of the wheat, and finally decreased with the senescence of the wheat. Rhopalosiphum maidis appeared early in the season, R. padi appeared late, Schizaphis graminum and Sitovion akebiae appeared intermittently in the season. S. graminum appeared more frequently on wheat than barley. DIMBOA was detected from wheat but not from barley. Gramine (N,N-dimethyl-indole-3-methanamine) was detected from barley, and is known as an important resistance substance. However gramine could not be detected in wheat. DIMBOA was found in higher amounts in young wheat, and gradually decreased with growth. A clear relationship between the aphid population and DIMBOA amounts could not be observed. However, all the wheat varieties used in this experiment seemed to have resistance against aphids. The resistance was compared with barley susceptible lines. DIMBOA was presumed to share the property of resistance with aconitic acid in wheat. |
| Keywords | DIMBOA content Wheat Aphid Resistant varaity Susceptible variety |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 17 |
| End Page | 26 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Increase in Cytochorome c and a 11.9 kDa protein in Submerged Rice Seedlings after Exposure to Air |
|---|---|
| FullText URL | 002_002_149_157.pdf |
| Author | Shibasaka, Mineo| Ushimaru, Takashi| Ookubo, Katsuyuki| Tsuchida, Shin-ichi| Tsuji, Hideo| |
| Abstract | To examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings. |
| Keywords | Air-adaptation Anti-cytochrome c serum Cytochrome c Cytocrome c purification Oryza sativa |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue2 |
| Start Page | 149 |
| End Page | 157 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Changes in Mitochondrial Spectra of Submerged Rice Seedlings after Exposure to Air Gaussian Deconvolution Analysis |
|---|---|
| FullText URL | 002_002_135_147.pdf |
| Author | Shibasaka, Mineo| Tsuji, Hideo| |
| Abstract | Quantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel. |
| Keywords | Absorption spectrum Air-adaptation Cytocrome Gaussian deconvolution analysis Oryza sativa |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue2 |
| Start Page | 135 |
| End Page | 147 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Estimation of Turbulent Fluxes by Gradient Method with Surface Temperatures of Rice Plant Canopy |
|---|---|
| FullText URL | 006_001_001_011.pdf |
| Author | Miyashita, Koichi| Maitani, Toshihiko| |
| Abstract | Micrometeorological observations including turbulent fluxes and canopy surface temperatures were made in paddy in the summer of 1997. The sensible and latent heat fluxes were estimated by the gradient method under the assumption of similarity between the turbulent exchange coefficient for momentum and that for heat and water vapor. The surface temperatures measured by a thermal imager and water vapor pressures estimated from surface temperatures and relative humidity were used for temperature and water vapor at the height(d+z0) of U=0, where d is the displacement height, z0 the roughness length and U the mean wind velocity. The sensible and latent heat fluxes estimated by the gradient method depicted typical diurnal varietions. The turbulent fluxes by the present method and eddy correlation method showed fairly good agreement, especially for latent heat fluxes. These results confirmed that this method is a promising one. |
| Keywords | Surface temperature Rice plant canopy Thermal imager Turbulent fluxes |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 11 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
|---|---|
| FullText URL | 003_002_117_127.pdf |
| Author | Yonetani, Tsutomu| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
| Keywords | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 117 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Author | Okamoto, K.| Ozawa, J.| |
|---|---|
| Published Date | 1962-03 |
| Publication Title | 農学研究 |
| Volume | volume49 |
| Issue | issue3 |
| Content Type | Departmental Bulletin Paper |
| Author | Okamoto, K.| Ozawa, J.| |
|---|---|
| Published Date | 1960-12 |
| Publication Title | 農学研究 |
| Volume | volume48 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Sakai, S.| Baba, I.| Ota, Y.| |
|---|---|
| Published Date | 1977-03 |
| Publication Title | 農学研究 |
| Volume | volume56 |
| Issue | issue4 |
| Content Type | Departmental Bulletin Paper |
| Author | Sakai, S.| Baba, I.| Ota, Y.| |
|---|---|
| Published Date | 1979-03 |
| Publication Title | 農学研究 |
| Volume | volume57 |
| Issue | issue3-4 |
| Content Type | Departmental Bulletin Paper |
| Author | Baba, I.| Sakai, S.| |
|---|---|
| Published Date | 1979-03 |
| Publication Title | 農学研究 |
| Volume | volume57 |
| Issue | issue3-4 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
|---|---|
| FullText URL | 003_002_163_174.pdf |
| Author | Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| Keywords | Odontoglossum ringspot virus Calanthe sp. Identification |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 163 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Author | Inouye, N.| Maeda, T.| Mitsuhata, K.| |
|---|---|
| Published Date | 1982-11 |
| Publication Title | 農学研究 |
| Volume | volume60 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe |
|---|---|
| FullText URL | 004_002_119_135.pdf |
| Author | Inouye, Narinobu| Matsumoto, Jun-ichi| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| |
| Abstract | Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV･So from Cymbidium. |
| Keywords | Calanthe Orchid fleck virus Calanthe yellowish fleck disease |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 119 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314037 |
| Title Alternative | Some Properties of Cymbidium Mosaic Virus Isolated from Calanthe spp. |
|---|---|
| FullText URL | 004_002_187_199.pdf |
| Author | Matsumoto, Jun-ichi| Urabe, Shinji| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| Inouye, Narinobu| |
| Abstract | Cymbidium mosaic virus(CyMV) was isolated from Calanthe spp. showing mosaic on the leaves, collected in Yamaguchi and Kyoto Prefectures in 1986～1993. CyMV, Cal. 90-1 isolate was transmitted by sapinoculation to 12 out of 37 species in 7 out of 9 families. Sap from diseaded Tetragonia expansa was infective to Chenopodium amaranticolor after dilution to 10-5 but not 10-6, after heating at 65℃ for 10 min but not 70℃, and after 1 month at 20℃ but not 2 months. The virus particles were flexuous rod, about 475 nm long. The virus was purified from diseased T. expansa leaves and contained a single protein species of Mr27,800. The Mr of the capsid proteins(Cal. 90-1) was similar to those of two ohter CyMV isolates(Cal. 90-4, Cal. 93-14).Cal. 90-1 and Cal. 93-14 reacted with antiserum to the Cymbidium isolate (Cy-16), suggesting that Cal. 90-1 was serologically very similar to the other two CyMV isolates. Two species of dsRNA were isolated from plants infected with Cal-1 and they were similar to those of two other CyMv isolates. |
| Keywords | Calanthe spp. Cymbidium mosaic virus Potexvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 187 |
| End Page | 199 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313956 |
| Author | Kuida, K.| Inouye, N.| Inouye, T.| |
|---|---|
| Published Date | 1977-02 |
| Publication Title | 農学研究 |
| Volume | volume56 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Tsuji, H.| Kawada, K.| |
|---|---|
| Published Date | 1985-03 |
| Publication Title | 農学研究 |
| Volume | volume60 |
| Issue | issue4 |
| Content Type | Departmental Bulletin Paper |
| Author | Tsuji, Hiroo| Kawada, Kazuo| |
|---|---|
| Published Date | 1989-03 |
| Publication Title | 農学研究 |
| Volume | volume61 |
| Issue | issue4 |
| Content Type | Departmental Bulletin Paper |
