| Title Alternative | ナメクジのα-グルコシダーゼの精製と性質 |
|---|---|
| FullText URL | 005_002_121_127.pdf |
| Author | Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| Keywords | Slug Incilaria bilineata α-glucosidase |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 121 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
|---|---|
| FullText URL | 001_002_159_166.pdf |
| Author | Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| Keywords | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1993 |
| Volume | volume1 |
| Issue | issue2 |
| Start Page | 159 |
| End Page | 166 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | メダカハネカクシの尾部分分泌物 |
|---|---|
| FullText URL | 004_001_025_031.pdf |
| Author | Kanehisa, Katsuo| Tsumuki, Hisaaki| |
| Abstract | Rove beetles have a pair of pygidial excretory organs, each group secretes characteristic substances in a peculiar manner. The genus Stenus has a pair of pygidial organs, eversible by immersion in diethyl-ether at both sides of the anus. S. anthoracinus, S. melanarius vercecundus and S. rufescens were collected at ponds and river shores, and S. alienus was collected at tobacco and vegetable fields. Excretory substances were secreted in the diethylether, and identified by gas chromatography and mass spectrometry. Stenusin [N-Ethyl-3-(2-methylbutyl) piperidine], 1,8-cineole, isopiperitenol and 6-Me-5-hepten-2-one were identified. These may probably act as defensive substances against small attacking animals and microorganisms, and act as water suface moving active agents. |
| Keywords | Rove beetles Stenus Excretory secretion Pygidial organ Defensive secretion |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 25 |
| End Page | 31 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | 2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出 |
|---|---|
| FullText URL | 005_001_023_030.pdf |
| Author | Maeda, Takanori| Sako, Nobumichi| Inouye, Narinobu| |
| Abstract | To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection. |
| Keywords | Cucumber mosaic virus Monoclonal antibody Simplified ELISA |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1997 |
| Volume | volume5 |
| Issue | issue1 |
| Start Page | 23 |
| End Page | 30 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | 幼苗期と成熟期のオオムギ系統間における禾穀類アブラムシの密度とグラミン含量の関係 |
|---|---|
| FullText URL | 004_001_049_058.pdf |
| Author | Moharramipour, Saeid| Murata, Shin-ichi| Kanehisa, Katsuo| Tsumuki, Hisaaki| |
| Abstract | The relationship between the indole alkaloid gramine concentration and aphid population was examined seedling and maturation stages in 14 barley lines of Hordeum spontaneum and H. unlgare. The density of Schizaphis graminum did not differ significantly with the gramine concentration in the seedling in the greenhouse. However, the population of Rhopalosiphum padi sometimes differed with the seedling. The plant resistance to the natural infestation of cereal aphids was obvious at the heading stage. There was a negative correlation between the high population density of aphids and gramine concentration. The gramine concentration was high in matured resistant resistant lines, especially wild lines, as compared with susceptible lines due to higher biodegradation activity. |
| Keywords | Barley Resistance Gramine Cereal aphids |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 49 |
| End Page | 58 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Author | Ozawa, J.| Okamoto, A.| Hayashi, T.| |
|---|---|
| Published Date | 1959-11 |
| Publication Title | 農学研究 |
| Volume | volume47 |
| Issue | issue1-2 |
| Content Type | Departmental Bulletin Paper |
| Author | Lim, J.| Yamasaki, Y.| Suzuki, Y.| Mino, A.| Ozawa, J.| |
|---|---|
| Published Date | 1981-02-25 |
| Publication Title | 農学研究 |
| Volume | volume59 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | 大麦網斑病抵抗性の選抜効果 |
|---|---|
| FullText URL | 003_001_043_053.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. |
| Keywords | Net blotch Pyrenophora teres Selection Barley Disease resistance |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 53 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120004537480 |
| Title Alternative | 高対塩性オオムギ品種の選抜 |
|---|---|
| FullText URL | 001_002_113_129.pdf |
| Author | Sopandie, Didy| Takeda, Kazuyoshi| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | In order to select higher salt tolerant cultivars of barley,solution culture experiments were carried out with 500 barley cultivars, which were preliminarily selected from 4581 cultivars by exposure to 1-2% of sodium chloride(MaCl) during the germinating stage. The concentrations of NaCl used in the experiments were 2 (control), 150 and 300 mM in nutrient solution.From the results of five solution culture experiments, each of which was carried out with about 100 cultivars, 105 cultivars of barley were selected as relatively about 100 cultivars. Then the final selection was carried out on those 105 cultivars, and it was found that more than 10 cultivars were highly salt tolerant, mainly based on the comparisons of shoot yields under the supply of 300 mM of NaCl.High salt tolerant barley cultivars mentioned above showed 42-86% and 17-30% of the control in shoot yield under the condition of 150 and 300 mM of NaCl, respectively.On three groups of barley,each having more than 10 cultivars, which are high, moderate and low in salt tolerance, ion contents of the plants were analyzed.The results suggest that potassium(K) uptake was comparatively larger in the high salt tolerant goup of barley cultivars,through there was a big variation in sodium(Na) and K contents even within the same group. |
| Keywords | Barley Ion content Salt tolerance Sodium chloride Yield |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1993 |
| Volume | volume1 |
| Issue | issue2 |
| Start Page | 113 |
| End Page | 129 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | エビネから分離されたインゲンマメ黄斑モザイクウイルスの諸性質 |
|---|---|
| FullText URL | 006_001_043_051.pdf |
| Author | Matsumoto, Jun-Ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Bean yellow mosaic virus (BYMV) was isolated from Calanthe sp.showing mild chlorosis on the leaves, collected in Yamaguchi Prefecture in Japan. The virus was transmitted by the aphid Myzus persicae in a non-persistent manner, and by sap-inoculation to 29 out of 46 plant species from 9 out of 12 families tested. Stability in crude sap, morphology of virus particles, shape of cylidrical inclusions and the presence of cytoplasmic crystalline inclusions in the infected cells were similar to those of BYMV isolates previously reported. The virus contained a single protein species with a molecular weight of 35,000. In a microprecipition test and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the virus showed a close serological relationship to isolates of BYMV from both crocus and gladiolus, and showed a distant relationship to clover yellow vein virus. Three BYMV isolates used in this study were found to be serologically related to each other, but the virus was more closely related to the BYMV crocus isolate than to gladiolus isolate. |
| Keywords | Calanthe sp. bean yellow mosaic virus clover yellow vein virus serological relationship |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1999 |
| Volume | volume6 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 51 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Author | Oshima, S.| |
|---|---|
| Published Date | 1959-11 |
| Publication Title | 農学研究 |
| Volume | volume47 |
| Issue | issue1-2 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | ゼニゴケ葉緑体遺伝子と相同性を持つラン藻 Synechocystis PCC6803株のORF326、frxC およびORF469を標的にした変異の導入 |
|---|---|
| FullText URL | 002_002_167_180.pdf |
| Author | Ogura, Yutaka| Takemura, Miho| Oda, Kenji| Ohyama, Kanji| |
| Abstract | ORF326, frxC and ORF469 of a transfomable cynobacterium, Synechcystis PCC6803, have sequence similarity with ORF465 on the choroplast genome of a livewort, Marchantia polymorpha, respectively. To elucidate their functions,targeted mutagenesis was performed by transformation with clened DNA in which the ORF was disrupted by insertion of a kanamycin resistancen gene cassette.Streak-purifications of a single colony of each transformant were repeatde to segregate homozygous mutants for disrupted copies, because Synechocystis PCC6803 was reported to have approximately 10 chromosomal DNA copies. Southern blot analysis revealed that mutants for ORF326 had not only disrupted ORF326 copies but also wild type ORF326 copies. This suggests that ORF326 is indispensable for growth under the mixotrophic growth condition used. However, mutants for frxC and mutants for ORF469 had only mutated copies, indicating that they dispensable for growth. Growth and chlorophyll a content of an ORF469-disrupted mutant were compared and chlorophyll a content of an ORF469-disrupted mutant were compared to those of wild type under mixotrophic growth condition, but no significant difference was detected. This indicates that ORF469 is required for neither normal growth nor chlorophyll biosynthesis under thie condition. |
| Keywords | Cyanobacteria Gene disruption Synechocystis PCC6803 |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue2 |
| Start Page | 167 |
| End Page | 180 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | トウモロコシに含まれるDIMBOAとアブラムシ抵抗性の関係 |
|---|---|
| FullText URL | 004_001_033_042.pdf |
| Author | Rustamaki, Maqsood A| Kanehisa, Katsuo| Tsumuki, Hisaaki| Shiraga, Takashi| |
| Abstract | 2,4-Dihydrox-7-methoxy1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid is considered to be one of the components responsible for the resistance to pest insects in cereal plants. The relationship between concentration of DIMBOA and aphid infestation on 21 corn lines was investigated in 1990 and 1991. DIMBOA was detected in leaves od all corn lines tested, contained larger amounts in young plants and gradually decreased with growth. This property was the same as observed in wheat. A more than ten times difference in DIMBOA concentration was observed in corn lines. However, there was no positive correlation between DIMBOA concentration and aphid density. Most of the lines used had resistance to aphids. Resistance may be built together with other components such as (E)-aconitic acid. |
| Keywords | Apfid Resistant substance Hydroxamic acid DIMBOA Corn |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 33 |
| End Page | 42 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Author | Kasahara, Y.| Nishi, K.| Ueyama, Y.| |
|---|---|
| Published Date | 1966-12 |
| Publication Title | 農学研究 |
| Volume | volume51 |
| Issue | issue3 |
| Content Type | Departmental Bulletin Paper |
| Author | Inouye, T.| Inouye, N.| |
|---|---|
| Published Date | 1964-03 |
| Publication Title | 農学研究 |
| Volume | volume50 |
| Issue | issue2 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
|---|---|
| FullText URL | 003_001_035_041.pdf |
| Author | Hou, Fwu-Fenn| Thseng, Fu-Sheng| Wu, Shu-Tu| Takeda, Kazuyoshi| |
| Abstract | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| Keywords | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 35 |
| End Page | 41 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Author | Inouye, T.| |
|---|---|
| Published Date | 1965-12 |
| Publication Title | 農学研究 |
| Volume | volume51 |
| Issue | issue1-2 |
| Content Type | Departmental Bulletin Paper |
| Title Alternative | Absorption of Iron by MuSty-Odor Producing Cyanobacteria (Anabaena macrospora, Phormidium tenue, Oscillatoria tenuis and Oscillatoria brevis) |
|---|---|
| FullText URL | 001_001_035_050.pdf |
| Author | Nakashima, Susumu| Aoyama, Isao| Yagi, Masakazu| |
| Abstract | 近年、琵琶湖の南湖をはじめ、霞々浦や相模湖など多くの湖沼や水道水源地において、人間活動に付随すると富栄養化問題となり、ラン藻類、特にかび臭物質を産生するラン藻の発生が憂慮されている。琵琶湖を水源とする大津、京都、大阪、神戸など関西の各都市の水道では、同湖で毎年発生するラン藻、すなわちAnabaena macrospora｛ジオスミン（geosmin）を産生｝やPhormidium tenue, Oscillatoria tenuis｛2-メチルイソボルネオール（2-methylisoborneol, MIB）を産生｝などが作り出すこれらのかび臭物質（ジオスミンまたはMIB）による水道水の異臭味が大きな問題となっている。こうした水圏環境における異臭味は世界各地でも問題となり、近年この問題に関する国際シンポジウム（International Symposium on Off-Flavors in the Aquatic Environment）が開かれるようになり、1982年6月には第1回（ヘルシンキ）、1987年10月には第2回（鹿児島）、そして1991年3月には第3回（ロサンゼルス）のシンポジウムがそれぞれ開催された。しかしこうしたラン藻が異常増殖する要因はまだ明らかではない。そのためかび臭物質を産生するラン藻類の発生を予測あるいは抑制する情報に関する研究は強い要請があるにもかかわらずあまり進展をみていない。 |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1992 |
| Volume | volume1 |
| Issue | issue1 |
| Start Page | 35 |
| End Page | 50 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Author | Kobayashi, J.| Morii, F.| Muramoto, S.| Nakashima, S.| |
|---|---|
| Published Date | 1976-02-28 |
| Publication Title | 農学研究 |
| Volume | volume55 |
| Issue | issue3 |
| Content Type | Departmental Bulletin Paper |
| Author | Inouye, N.| Mitsuhata, K.| |
|---|---|
| Published Date | 1978-02 |
| Publication Title | 農学研究 |
| Volume | volume57 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
