JaLCDOI 10.18926/AMO/54987
FullText URL 71_2_179.pdf
Author Nosaka, Nobuyuki| Tsukahara, Kohei| Knaup, Emily| Yabuuchi, Toshihiko| Kikkawa, Tomonobu| Fujii, Yosuke| Yashiro, Masato| Yasuhara, Takao| Okada, Ayumi| Ugawa, Toyomu| Nakao, Atsunori| Tsukahara, Hirokazu| Date, Isao|
Abstract Newly published clinical practice guidelines recommend intracranial pressure (ICP) monitoring in critical care for the management of pediatric acute encephalopathy (pAE), but the utility of ICP monitoring for pAE has been poorly studied. We recently performed direct ICP monitoring for two patients. We observed that although the direct ICP monitoring had clinical benefits with less body weight gain and no vasopressor use in both cases, this monitoring technique is still invasive. Future studies should determine the utility of non-invasive ICP monitoring systems in pAE to further improve the quality of intensive-care management.
Keywords cerebral perfusion encephalopathy child intracranial pressure neurological intensive care
Amo Type Short Communication
Published Date 2017-04
Publication Title Acta Medica Okayama
Volume volume71
Issue issue2
Publisher Okayama University Medical School
Start Page 179
End Page 180
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28420900
JaLCDOI 10.18926/AMO/53674
FullText URL 69_5_279.pdf
Author Saito, Yukie| Fujii, Yousuke| Yashiro, Masato| Tsuge, Mitsuru| Nosaka, Nobuyuki| Yamashita, Nobuko| Yamada, Mutsuko| Tsukahara, Hirokazu| Morishima, Tsuneo|
Abstract Lung hyperpermeability affects the development of acute respiratory distress syndrome (ARDS), but therapeutic strategies for the control of microvascular permeability have not been established. We examined the effects of edaravone, dexamethasone, and N-monomethyl-L-arginine (L-NMMA) on permeability changes in human pulmonary microvascular endothelial cells (PMVEC) under a hypercytokinemic state. Human PMVEC were seeded in a Boyden chamber. After monolayer confluence was achieved, the culture media were replaced respectively by culture media containing edaravone, dexamethasone, and L-NMMA. After 24-h incubation, the monolayer was stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Fluorescein-labeled dextran was added. Then the trans-human PMVEC leak was measured. Expressions of vascular endothelial-cadherin (VE-cadherin) and zonula occludens-1 protein (ZO-1) were evaluated using real-time quantitative polymerase chain reaction and immunofluorescence microscopy. The results showed that TNF-α+IL-1β markedly increased pulmonary microvascular permeability. Pretreatment with edaravone, dexamethasone, or L-NMMA attenuated the hyperpermeability and inhibited the cytokine-induced reduction of VE-cadherin expression on immunofluorescence staining. Edaravone and dexamethasone increased the expression of ZO-1 at both the mRNA and protein levels. Edaravone and dexamethasone inhibited the permeability changes of human PMVEC, at least partly through an enhancement of VE-cadherin. Collectively, these results suggest a potential therapeutic approach for intervention in patients with ARDS.
Keywords pulmonary microvascular endothelial cells permeability edaravone vascular endothelial-cadherin zonula occludens-1 protein
Amo Type Original Article
Published Date 2015-10
Publication Title Acta Medica Okayama
Volume volume69
Issue issue5
Publisher Okayama University Medical School
Start Page 279
End Page 290
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 26490025
Web of Science KeyUT 000365519600004
Author Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi| Yodoi, Junji| Morishima, Tsuneo|
Published Date 2013-08-01
Publication Title 岡山医学会雑誌
Volume volume125
Issue issue2
Content Type Journal Article
Author Nakatsukasa, Yoko| Tsukahara, Hirokazu| Tabuchi, Kazuhisa| Tabuchi, Masako| Magami, Tomoko| Yamada, Mutsuko| Fujii, Yosuke| Yashiro, Masato| Tsuge, Mitsuru| Morishima, Tsuneo|
Published Date 2013-01-01
Publication Title Journal of Clinical Biochemistry and Nutrition
Volume volume52
Issue issue1
Content Type Journal Article
Author Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi|
Published Date 2013-01
Publication Title Critical Care Medicine
Volume volume41
Issue issue1
Content Type Journal Article