Author Baba, Naoko| Sakaguchi, Hiromichi| Taguchi, Takaaki| Hayase, Nobuki| Tamura, Takashi| Inagaki, Kenji|
Published Date 2006-02
Publication Title 岡山大学農学部学術報告
Volume volume95
Issue issue1
Content Type Departmental Bulletin Paper
Author Kuwahara, Mitsuhiko| Tamura, Takashi| Kawamura, Kentaro| Inagaki, Kenji|
Published Date 2007-02
Publication Title 岡山大学農学部学術報告
Volume volume96
Issue issue1
Content Type Departmental Bulletin Paper
Author Tashiro (Yamada), Yuriko| Tamura, Takashi| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2004-02
Publication Title 岡山大学農学部学術報告
Volume volume93
Issue issue1
Content Type Departmental Bulletin Paper
Author Yamaoka, Seiji| Tamura, Takashi| Takenobu, Hisanori| Kojo, Toshiyuki| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2003-02
Publication Title 岡山大学農学部学術報告
Volume volume92
Issue issue1
Content Type Departmental Bulletin Paper
Author Tamura, Takashi| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2003-02
Publication Title 岡山大学農学部学術報告
Volume volume92
Issue issue1
Content Type Departmental Bulletin Paper
Author Tamura, Takashi| Li, Yin Shu| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2002-02
Publication Title 岡山大学農学部学術報告
Volume volume91
Issue issue1
Content Type Departmental Bulletin Paper
Author Takahashi, Tatsuya| Tanaka, Noboru| Tamura, Takashi| Mukai, Chika| Isobe, Kimiyasu| Wakao, Norio| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2002-02
Publication Title 岡山大学農学部学術報告
Volume volume91
Issue issue1
Content Type Departmental Bulletin Paper
Author Arima, Jiro| Tamura, Takashi| Shinohara, Hiroaki| Kusakabe, Hitoshi| Tanaka, Hidehiko| Inagaki, Kenji|
Published Date 2002-02
Publication Title 岡山大学農学部学術報告
Volume volume91
Issue issue1
Content Type Departmental Bulletin Paper
Author Inagaki, Kenji| Kawaguchi, Masakazu| Taguchi, Takaaki| Tamura, Takashi| Tanaka, Hidehiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Inoue, Hiroyuki| Tamura, Takashi| Inagaki, Kenji| Tanaka, Hidehiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Seow, Teck Keong| Inagaki, Kenji| Tamura, Takashi| Tanaka, Hidehiko|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Tamura, Takashi|
Published Date 1995-02-01
Publication Title 岡山大学農学部学術報告
Volume volume84
Issue issue1
Content Type Departmental Bulletin Paper
Author Fukuda, Koji| Tamura, Takashi| Inagaki, Kenji|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Shinozaki, Mai| Yanagitani, Masahiko| Kaneda, Shouichirou| Kudou, Daizou| Endou, Yuuichi| Tamura, Takashi| Kuramitsu, Seiki| Inagaki, Kenji|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Takahata, Muneaki| Tamura, Takashi| Abe, Katsumasa| Mihara, Hisaaki| Kurokawa, Suguru| Yamamoto, Yoshihiro| Nakano, Ryuhei| Esaki, Nobuyoshi| Inagaki, Kenji|
Published Date 2008-06-26
Publication Title Journal of Biochemistry
Volume volume143
Issue issue4
Content Type Journal Article
Title Alternative Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
FullText URL srfa_109_001_006.pdf
Author Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji|
Abstract  Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Keywords glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 1
End Page 6
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL fulltext.pdf
Author Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori|
Keywords Biomineralization Diatom Silica Transcriptome Proteome
Note This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.|
Published Date 2020-06-03
Publication Title Marine Biotechnology
Publisher Springer
ISSN 1436-2228
NCID AA11357643
Content Type Journal Article
language French
OAI-PMH Set 岡山大学
File Version author
PubMed ID 32488507
DOI 10.1007/s10126-020-09976-1
Web of Science KeyUT 000537432000001
Related Url isVersionOf https://doi.org/10.1007/s10126-020-09976-1
Author Kobayashi, Fumiaki| Aomine, Hiroki| Mizunashi, Wataru| Yu, Fujio| Tamura, Takashi| Inagaki, Kenji|
Published Date 2012-02-01
Publication Title 岡山大学農学部学術報告
Volume volume101
Content Type Departmental Bulletin Paper
Author Tachibana, Akiko| Tamura, Takashi| Imada, Katsumi| Kinoshita, Miki| Namba, Keiichi| Tsutsumi, Noriko| Hashida, Miyoko| Sakaguchi, Hiromichi| Inagaki, Kenji|
Published Date 2008-02
Publication Title 岡山大学農学部学術報告
Volume volume97
Issue issue1
Content Type Departmental Bulletin Paper
FullText URL fulltext20210530_4.pdf
Author Kitagawa, Masaki| Ito, Nanako| Matsumoto, Yuya| Saito, Masaya| Tamura, Takashi| Kusakabe, Hitoshi| Inagaki, Kenji| Imada, Katsumi|
Keywords L-Lysine α-oxidase Crystal structure Precursor Substrate recognition
Published Date 2021-12-31
Publication Title Journal of Structural Biology: X
Volume volume5
Publisher Elsevier
Start Page 100044
ISSN 25901524
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2021 The Author(s).
File Version publisher
PubMed ID 33554108
DOI 10.1016/j.yjsbx.2021.100044
Related Url isVersionOf https://doi.org/10.1016/j.yjsbx.2021.100044