ID | 10604 |
Eprint ID | 10604
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FullText URL | |
Title Alternative | Glutamate Snsor Using L-Glutamate Oxidase and Its Application for Sensing GOT/GPT Activity
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Author |
Arima, Jiro
Shinohara, Hiroaki
Kusakabe, Hitoshi
Tanaka, Hidehiko
Inagaki, Kenji
Kaken ID
researchmap
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Abstract | L-Glutamate measurement and GOT/GPT assay was successful by H2O2 measurement using the L-glutamate oxidase with 4-aminoantipyrine / phenol method. But, in examination of oxigen electrode, immobilized L-glutamate oxidase at the cellulose to L-glutamate and GOT-GPT sensor, Lpglutamate measurement was used for the amperometric determination with non-fixed enzyme. On examination of electron mediator, response for L-glutamate was observed with each of the compounds ferricyane, ferrocene-COOH, ferrocene-MeOH, and benzoquinone. L-Glutamate was measured by carbon printed tip electrode the L-glutamate oxidase and ferricyane based on the principle of chronoamperometry. A linear calibration graph was obtained between 1mM and 30mM. These results suggest that L-glutamate oxidase is able to utilize to L-glutamate sensor, and that there is a strong possibility to put this sensor to sensing for GOT/GPT activity.
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Keywords | L-glutamate ocidase
L-glutamate sensor
GOT/GPT sensor
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Published Date | 2002-02
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Publication Title |
岡山大学農学部学術報告
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Publication Title Alternative | Scientific reports of the Faculty of Agriculture, Okayama University
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Volume | volume91
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Issue | issue1
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Publisher | 岡山大学農学部
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Publisher Alternative | Faculty of Agriculture,Okayama University
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Start Page | 15
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End Page | 22
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ISSN | 0474-0254
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NCID | AN00033029
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Content Type |
Departmental Bulletin Paper
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language |
Japanese
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File Version | publisher
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Refereed |
False
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Eprints Journal Name | srfa
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