Author Okada, Koki| Morimoto, Yu| Shiraishi, Yukine| Tamura, Takashi| Mayama, Shigeki| Kadono, Takashi| Adachi, Masao| Ifuku, Kentaro| Nemoto, Michiko|
Keywords Diatom Genetic transformation Nitzschia Multi-pulse electroporation
Note This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10126-023-10273-w| This fulltext file will be available in Dec. 2024.|
Published Date 2023-12-10
Publication Title Marine Biotechnology
Volume volume25
Issue issue6
Publisher Springer Science and Business Media LLC
Start Page 1208
End Page 1219
ISSN 1436-2228
NCID AA11357643
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023
File Version author
PubMed ID 38071657
DOI 10.1007/s10126-023-10273-w
Web of Science KeyUT 001120097200001
Related Url isVersionOf https://doi.org/10.1007/s10126-023-10273-w
FullText URL fulltext.pdf
Author Jaunet-Lahary, Titouan| Shimamura, Tatsuro| Hayashi, Masahiro| Nomura, Norimichi| Hirasawa, Kouta| Shimizu, Tetsuya| Yamashita, Masao| Tsutsumi, Naotaka| Suehiro, Yuta| Kojima, Keiichi| Sudo, Yuki| Tamura, Takashi| Iwanari, Hiroko| Hamakubo, Takao| Iwata, So| Okazaki, Kei-Ichi| Hirai, Teruhisa| Yamashita, Atsuko|
Note The version of record of this article, first published in Nature Communications, is available online at Publisher’s website: http://dx.doi.org/10.1038/s41467-023-36883-5|
Published Date 2023-04-03
Publication Title Nature Communications
Volume volume14
Issue issue1
Publisher Nature Portfolio
Start Page 1730
ISSN 2041-1723
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2023
File Version publisher
PubMed ID 37012268
DOI 10.1038/s41467-023-36883-5
Web of Science KeyUT 001002031500007
Related Url isVersionOf https://doi.org/10.1038/s41467-023-36883-5
FullText URL fulltext20210530_4.pdf
Author Kitagawa, Masaki| Ito, Nanako| Matsumoto, Yuya| Saito, Masaya| Tamura, Takashi| Kusakabe, Hitoshi| Inagaki, Kenji| Imada, Katsumi|
Keywords L-Lysine α-oxidase Crystal structure Precursor Substrate recognition
Published Date 2021-12-31
Publication Title Journal of Structural Biology: X
Volume volume5
Publisher Elsevier
Start Page 100044
ISSN 25901524
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2021 The Author(s).
File Version publisher
PubMed ID 33554108
DOI 10.1016/j.yjsbx.2021.100044
Related Url isVersionOf https://doi.org/10.1016/j.yjsbx.2021.100044
FullText URL fulltext.pdf
Author Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori|
Keywords Biomineralization Diatom Silica Transcriptome Proteome
Note This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.|
Published Date 2020-06-03
Publication Title Marine Biotechnology
Publisher Springer
ISSN 1436-2228
NCID AA11357643
Content Type Journal Article
language French
OAI-PMH Set 岡山大学
File Version author
PubMed ID 32488507
DOI 10.1007/s10126-020-09976-1
Web of Science KeyUT 000537432000001
Related Url isVersionOf https://doi.org/10.1007/s10126-020-09976-1
Title Alternative Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
FullText URL srfa_109_001_006.pdf
Author Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji|
Abstract  Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Keywords glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 1
End Page 6
ISSN 2186-7755
language Japanese
File Version publisher
Author Tamura, T.| Ibi, T.| Inagaki, K.| Kubo, Y.| Okuda, K.|
Published Date 2016-02-01
Publication Title 岡山大学農学部学術報告
Volume volume105
Content Type Departmental Bulletin Paper
Author Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi|
Published Date 2016-01-28
Publication Title Scientific reports
Volume volume6
Content Type Journal Article
Author Oshima, Kenshiro| Hattori, Masahira| Shimizu, Hitomi| Fukuda, Koji| Nemoto, Michiko| Inagaki, Kenji| Tamura, Takashi|
Published Date 2015-07-09
Publication Title Genome Announcements
Volume volume3
Issue issue4
Content Type Journal Article
Author Nakai, Ryuichiro| Fujino, Shihoko| Utsumi, Tomohiro| Tamura, Takashi| Kusakabea, Hitoshi| Inagaki, Kenji|
Published Date 2014-02-01
Publication Title 岡山大学農学部学術報告
Volume volume103
Content Type Departmental Bulletin Paper
Author Kobayashi, Fumiaki| Aomine, Hiroki| Mizunashi, Wataru| Yu, Fujio| Tamura, Takashi| Inagaki, Kenji|
Published Date 2012-02-01
Publication Title 岡山大学農学部学術報告
Volume volume101
Content Type Departmental Bulletin Paper
Author Maemura, Tomomi| Uchitomi, Kumiko| Kusaka, Chika| Inagaki, Junko| Tamura, Takashi| Soda, Kenji| Inagaki, Kenji|
Published Date 2011-02-01
Publication Title 岡山大学農学部学術報告
Volume volume100
Content Type Departmental Bulletin Paper
Author Takahata, Muneaki| Tamura, Takashi| Abe, Katsumasa| Mihara, Hisaaki| Kurokawa, Suguru| Yamamoto, Yoshihiro| Nakano, Ryuhei| Esaki, Nobuyoshi| Inagaki, Kenji|
Published Date 2008-06-26
Publication Title Journal of Biochemistry
Volume volume143
Issue issue4
Content Type Journal Article
Author Shinozaki, Mai| Yanagitani, Masahiko| Kaneda, Shouichirou| Kudou, Daizou| Endou, Yuuichi| Tamura, Takashi| Kuramitsu, Seiki| Inagaki, Kenji|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Fukuda, Koji| Tamura, Takashi| Inagaki, Kenji|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Departmental Bulletin Paper
Author Yanagitani, Masahiko| Uemae, Satoshi| Shiraga, Tomoyuki| Tamura, Takashi| Inagaki, Kenji|
Published Date 2009-02
Publication Title 岡山大学農学部学術報告
Volume volume98
Issue issue1
Content Type Departmental Bulletin Paper
Author Tachibana, Akiko| Tamura, Takashi| Imada, Katsumi| Kinoshita, Miki| Namba, Keiichi| Tsutsumi, Noriko| Hashida, Miyoko| Sakaguchi, Hiromichi| Inagaki, Kenji|
Published Date 2008-02
Publication Title 岡山大学農学部学術報告
Volume volume97
Issue issue1
Content Type Departmental Bulletin Paper
Author Tamura, Takashi|
Published Date 1995-02-01
Publication Title 岡山大学農学部学術報告
Volume volume84
Issue issue1
Content Type Departmental Bulletin Paper
Author Seow, Teck Keong| Inagaki, Kenji| Tamura, Takashi| Tanaka, Hidehiko|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Inoue, Hiroyuki| Tamura, Takashi| Inagaki, Kenji| Tanaka, Hidehiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper
Author Inagaki, Kenji| Kawaguchi, Masakazu| Taguchi, Takaaki| Tamura, Takashi| Tanaka, Hidehiko|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper