ID | 65933 |
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Author |
Ohmori, Iori
Section of Developmental Physiology and Pathology, Faculty of Education, Okayama University
ORCID
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Ouchida, Mamoru
Department of Molecular Oncology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Shinohara, Masakazu
Division of Epidemiology, Kobe University Graduate School of Medicine
Kobayashi, Kiyoka
Otsuka Pharmaceutical
Ishida, Saeko
Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo
Mashimo, Tomoji
Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo
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Abstract | Thioredoxin, encoded by Txn1, is a critical antioxidant that protects against oxidative damage by regulating the dithiol/disulfide balance of interacting proteins. We recently discovered the Adem rat, an epileptic rat harboring the Txn1-F54L mutation, characterized by wild running and vacuolar degeneration in the midbrain. This study aimed to characterize the classification of epilepsy in Adem rats. We performed simultaneous video-electroencephalographic recordings, magnetic resonance imaging, neurotransmitter measurements using gas chromatography–mass spectrometry (GC-MS), and immunohistochemistry. Adem rats exhibited absence, tonic, and focal seizures. The type of epilepsy was classified as combined generalized and focal epilepsy. Neurotransmitters in the midbrain and cortex were measured at 3 weeks of age, when neuronal cell death occurs in the midbrain. The results of GC-MS ruled out the dominance of the excitatory system in the midbrain and cortex of Adem rats. Activation of astrocytes and microglia was more pronounced at 5 weeks of age, at which time epileptic seizures occurred frequently. The underlying pathology in Adem rats remains unknown. However, glial cell activation and inflammation may play a significant role in the occurrence of epilepsy.
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Keywords | animal model
combined generalized and focal epilepsy
oxidative stress
thioredoxin
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Published Date | 2022-05-28
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Publication Title |
Epilepsia
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Volume | volume63
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Issue | issue7
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Publisher | Wiley
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Start Page | e80
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End Page | e85
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ISSN | 0013-9580
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 The Authors.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1111/epi.17295
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License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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Citation | Ohmori, I, Ouchida, M, Shinohara, M, Kobayashi, K, Ishida, S, Mashimo, T. Novel animal model of combined generalized and focal epilepsy. Epilepsia. 2022; 63: e80–e85. https://doi.org/10.1111/epi.17295
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 16H05354
JP16H06276
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