JaLCDOI | 10.18926/AMO/31296 |
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FullText URL | fulltext.pdf |
Author | Takeuchi, Kazuhiro| Inoue, Hajime| Yokoyama, Yoshiki| Senda, Masuo| Ota, Yusuke| Abe, Nobuhiro| Nishida, Keiichiro| |
Abstract | We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment. |
Keywords | rheumatoid arthritis magnetic resonance imaging scoring system synovial membrane |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1998-08 |
Volume | volume52 |
Issue | issue4 |
Publisher | Okayama University Medical School |
Start Page | 211 |
End Page | 224 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 9781272 |
Web of Science KeyUT | 000075623600006 |
JaLCDOI | 10.18926/AMO/30974 |
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FullText URL | fulltext.pdf |
Author | Ikuma, Hisanori| Abe, Nobuhiro| Uchida, Youichiro| Furumatsu, Takayuki| Fujiwara, Kazuo| Nishida, Keiichiro| Ozaki, Toshifumi| |
Abstract | Instability of the knee after the medial collateral ligament (MCL) injury is usually assessed with the manual valgus stress test, even though, in recent years, it has become possible to apply magnetic resonance imaging (MRI) to the assessment of the damage of the ligament. The valgus instability of 24 patients (12 isolated injuries and 12 multiple ligament injuries) who suffered MCL injury between 1993 and 1998 was evaluated with the Hughston and Eilers classification, which involves radiographic assessment under manual valgus stress to the injured knees. We developed a novel system for classifying the degree of injury to the MCL by calculating the percentage of injured area based on MRI and investigated the relationship between this novel MRI classification and the magnitude of valgus instability by the Hughston and Eilers classification. There was a significant correlation between the 2 classifications (p=0.0006). On the other hand, the results using other MRI based classification systems, such as the Mink and Deutsch classificaiton and the Petermann classification, were not correlated with the findings by the Hughston and Eilers classification in these cases (p0.05). Since MRI is capable of assessing the injured ligament in clinical practice, this novel classification system would be useful for evaluating the stability of the knee and choosing an appropriate treatment following MCL injury. |
Keywords | medial collateral ligament magnetic resonance imaging knee instability novel method |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2008-06 |
Volume | volume62 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 185 |
End Page | 191 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 18596835 |
Web of Science KeyUT | 000257130300006 |
JaLCDOI | 10.18926/AMO/30956 |
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FullText URL | fulltext.pdf |
Author | Doi, Hideyuki| Nishida, Keiichiro| Yorimitsu, Masanori| Komiyama, Takamitsu| Kadota, Yasutaka| Tetsunaga, Tomonori| Yoshida, Aki| Kubota, Satoshi| Takigawa, Masaharu| Ozaki, Toshifumi| |
Abstract | Mechanical stress plays a key role in the pathogenesis of cartilage destruction seen in osteoarthritis (OA). We investigated the effect of cyclic tensile stress (CTS) on the anabolic and catabolic gene expression of rat cultured normal chondrocytes using the Flexercell strain unit. The effects of interleukin (IL)-4, a chondroprotective cytokine, on the changes in gene expression induced by CTS were also investigated. CTS (7% elongation at 0.5 Hz) for 24 h did not affect the expression of aggrecan and type II collagen, whereas CTS significantly upregulated matrix metalloproteinase (MMP)-13 and cathepsin B mRNA expression by chondrocytes. IL-1beta expression was also signifi cantly upregulated by CTS up to 12 h. The upregulation of MMP-13 was observed at 3 h, which was earlier than that of IL-1beta. Furthermore, pre-treatment with IL-4 (10 ng/ml) suppressed both MMP-13 and cathepsin B induction by mechanical stress, as well as CTS-induced IL-1beta expression. Our results suggest that IL-4 might have a therapeutic value in the treatment of OA by downregulation of mechanical stress-induced MMP-13 and cathepsin B expression by chondrocytes. |
Keywords | IL-4 MMP cathepsin B mechanical stress aggrecanase |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2008-04 |
Volume | volume62 |
Issue | issue2 |
Publisher | Okayama University Medical School |
Start Page | 119 |
End Page | 126 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 18464888 |
Web of Science KeyUT | 000255297600008 |
JaLCDOI | 10.18926/AMO/30794 |
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FullText URL | fulltext.pdf |
Author | Nagoshi, Mitsuru| Hashizume, Hiroyuki| Nishida, Keiichiro| Takagoshi, Hidekazu| Pu, Jean| Inoue, Hajime| |
Abstract | Sixty-seven trigger fingers of 58 idiopathic and hemodialysis patients were treated by percutaneous A1-pulley release technique. Severity of triggering was classified into five grades for treatment selection and prediction of possible results. Results were excellent in 41 fingers, good in 9, fair in 7, and poor in 10, requiring additional treatment. The results of the lower grades were better, and those of the higher grades were poor. Excellent or good results appeared to depend on the proper selection of the patients according to the grading system and confirmation of triggering disappearance just after the release. There were neither infections nor neuro-vascular deficits after treatment. Compared to conventional open release, this treatment was found to be more useful from the standpoints of ease and safety of the technique, and the patients' quick return to normal life. |
Keywords | trigger finger percutaneous release idiopathic hemodialysis |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1997-06 |
Volume | volume51 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 155 |
End Page | 158 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 9227795 |
Web of Science KeyUT | A1997XJ12700007 |
JaLCDOI | 10.18926/AMO/30790 |
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FullText URL | fulltext.pdf |
Author | Nanba, Yoshifumi| Nishida, Keiichiro| Yoshikawa, Takeshi| Sato, Toru| Inoue, Hajime| Kuboki, Yoshinori| |
Abstract | The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA. |
Keywords | chondrocalcinosis osteoarthritis osteonection Mankin's histologic-histochemical grading calcification |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1997-10 |
Volume | volume51 |
Issue | issue5 |
Publisher | Okayama University Medical School |
Start Page | 239 |
End Page | 243 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 9359920 |
Web of Science KeyUT | A1997YD65300001 |
JaLCDOI | 10.18926/AMO/30778 |
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FullText URL | fulltext.pdf |
Author | Kinugasa, Kiyoto| Hashizume, Hiroyuki| Nishida, Keiichiro| Shigeyama, Yukio| Inoue, Hajime| |
Abstract | The results of the histological examinations of specimens of the tenosynovium of the flexor tendon, the epineurium and the transverse carpal ligament from two groups of Japanese patients with carpal tunnel syndrome (idiopathic and hemodialysis) were compared. Amyloid deposits, positively identified as β2-microglobulin, appeared in all patients in the long-term hemodialysis group, but in no patients in the idiopathic group. Although the pathogenesis differed between the two groups, both resulted in nerve compression in the carpal tunnel. Therefore, surgical release is considered beneficial for both groups. |
Keywords | carpal tunnel syndrome histopathology clinical results idiopathic hemodialysis |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1997-04 |
Volume | volume51 |
Issue | issue2 |
Publisher | Okayama University Medical School |
Start Page | 63 |
End Page | 70 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 9142342 |
Web of Science KeyUT | A1997WX19600002 |
JaLCDOI | 10.18926/AMO/30753 |
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FullText URL | fulltext.pdf |
Author | Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji| |
Abstract | We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy. |
Keywords | colloidal iron microwave histochemistry transmission electron microscopy |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 2006-02 |
Volume | volume60 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 59 |
End Page | 64 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 16508690 |
Web of Science KeyUT | 000235538900007 |
JaLCDOI | 10.18926/AMO/30749 |
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FullText URL | fulltext.pdf |
Author | Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi| |
Abstract | Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p<0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR. |
Keywords | spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 2006-06 |
Volume | volume60 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 141 |
End Page | 148 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 16838042 |
Web of Science KeyUT | 000238503600001 |
JaLCDOI | 10.18926/AMO/30384 |
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FullText URL | fulltext.pdf |
Author | Nishida, Keiichiro| Inoue, Hajime| Toda, Kazukiyo| Murakami, Takuro| |
Abstract | Localization of the glycosaminoglycans (GAG) was examined in the synovial membranes of patients with osteoarthritis under light microscopy using a fine cationic colloidal iron staining method combined with enzymatic digestion. Our staining method was very useful for demonstrating the difference in the localization of GAG in regions of the inflammatory site in the osteoarthritic synovial membrane. Hyaluronic acid was mainly located in connective tissues in the surface intercellular and perivascular spaces, chondroitin sulfate A/C in the highly fibrous part of and connective tissue around blood vessels, dermatan sulfate (chondroitin sulfate B) in the subsurface interstitium and vascular endothelial cells and heparan sulfate in part of vascular endothelial cells. No keratan sulfate was detected. GAG is reported to have an important role in cell movement, adherence and aggregation in the inflammatory sites. These findings should be useful for understanding the role of GAG in physiological and pathologic processes of secondary synovitis. |
Keywords | glycosaminoglycan synovial tissue osteoarthritis fine cationic colloidal iron |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1995-12 |
Volume | volume49 |
Issue | issue6 |
Publisher | Okayama University Medical School |
Start Page | 287 |
End Page | 294 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 8770237 |
Web of Science KeyUT | A1995TM84600003 |