| FullText URL | fulltext.pdf |
|---|---|
| Author | Munetomo, Sosuke| Uchiyama, Jumpei| Takemura-Uchiyama, Iyo| Wanganuttara, Thamonwan| Yamamoto, Yumiko| Tsukui, Toshihiro| Hagiya, Hideharu| Kanamaru, Shuji| Kanda, Hideyuki| Matsushita, Osamu| |
| Published Date | 2024-10-23 |
| Publication Title | PLoS ONE |
| Volume | volume19 |
| Issue | issue10 |
| Publisher | Public Library of Science |
| Start Page | e0310962 |
| ISSN | 1932-6203 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2024 Munetomo et al. |
| File Version | publisher |
| PubMed ID | 39441843 |
| DOI | 10.1371/journal.pone.0310962 |
| Web of Science KeyUT | 001345720700068 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0310962 |
| JaLCDOI | 10.18926/AMO/67657 |
|---|---|
| FullText URL | 78_5_371.pdf |
| Author | Gotoh, Kazuyoshi| Miyoshi, Makoto| I Putu Bayu Mayura| Tsuji, Shuma| Iio, Koji| Fukushima, Shinnosuke| Matsushita, Osamu| Hagiya, Hideharu| |
| Abstract | Spread of carbapenemase-producing Enterobacterales (CPE) is an ongoing public health issue worldwide, including in Japan. In this study, we investigated the phenotypic and genetic characteristics of CPE isolates at Okayama University Hospital over the 5 years (2013-2018) prior to the outbreak of the 2019 coronavirus pandemic. Of 24 carbapenem-resistant Enterobacterales isolated during the study period, we identified 8 CPE isolates harboring blaIMP-1 (5 isolates) and blaIMP-6 genes (3 isolates). Bacterial species and carbapenem susceptibility patterns exhibited diversity. Minimum inhibitory concentrations (MICs) of meropenem were generally higher than those of imipenem and biapenem. Results of pulsed-field gel electrophoresis demonstrated that neither clonal nor plasmid-mediated outbreaks of blaIMP-harboring CPE isolates have developed at our hospital. One Klebsiella oxytoca isolate showed a high MIC (128 μg/mL) of meropenem, which could be explained by the high plasmid copy number. Subsequent analysis of this isolate may elucidate the intricacies of carbapenem resistance profiles among CPE isolates. Collectively, our findings underscore the necessity for ongoing genetic surveillance of CPE, complemented by tailored approaches for infection prevention and control. |
| Keywords | antimicrobial resistance carbapenemase-producing enterobacterales carbapenemase-resistant enterobacterales Silent pandemic whole genome sequence |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2024-10 |
| Volume | volume78 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 371 |
| End Page | 376 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2024 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| Author | Tsuji, Shuma| Gotoh, Kazuyoshi| Manabe, Tadahiro| Iio, Koji| Fukushima, Shinnosuke| Matsushita, Osamu| Hagiya, Hideharu| |
|---|---|
| Keywords | Beta-lactamase blaZ Cefazolin Inoculum effect Staphylococcus aureus |
| Note | © 2024 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/| This fulltext file will be available in Jun. 2025.| |
| Published Date | 2024-09 |
| Publication Title | Diagnostic Microbiology and Infectious Disease |
| Volume | volume110 |
| Issue | issue1 |
| Publisher | Elsevier BV |
| Start Page | 116399 |
| ISSN | 0732-8893 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2024 Elsevier Inc. |
| File Version | author |
| PubMed ID | 38875894 |
| DOI | 10.1016/j.diagmicrobio.2024.116399 |
| Web of Science KeyUT | 001255589200001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.diagmicrobio.2024.116399 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Yagisawa, Takuya| Uchiyama, Jumpei| Takemura-Uchiyama, Iyo| Ando, Shun| Ichii, Osamu| Murakami, Hironobu| Matsushita, Osamu| Katagiri, Seiji| |
| Keywords | dairy cows low fertility uterine microbiota microbial diversity bacterial association |
| Published Date | 2023-04-26 |
| Publication Title | Microbiology Spectrum |
| Volume | volume11 |
| Issue | issue3 |
| Publisher | American Society for Microbiology |
| Start Page | e04764-22 |
| ISSN | 2165-0497 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 Yagisawa et al. |
| File Version | publisher |
| PubMed ID | 37098918 |
| DOI | 10.1128/spectrum.04764-22 |
| Web of Science KeyUT | 000975042800001 |
| Related Url | isVersionOf https://doi.org/10.1128/spectrum.04764-22 |
| JaLCDOI | 10.18926/AMO/64355 |
|---|---|
| FullText URL | 77_1_1.pdf |
| Author | Nahar, Lutfun| Hagiya, Hideharu| Nada, Takahiro| Iio, Koji| Gotoh, Kazuyoshi| Matsushita, Osamu| Otsuka, Fumio| |
| Abstract | Inducible resistance to the macrolide, lincosamide, and streptogramin B (iMLSB) antibiotic family is a latent mechanism for antimicrobial resistance in Staphylococcus aureus. We here investigated the frequency and genotypic profiles of iMLSB resistance in clindamycin (CLDM)-susceptible S. aureus isolated in Okayama University Hospital from June 2020 to June 2021. We phenotypically screened the iMLSB resistance via D-zone test and performed PCR testing for the erythromycin ribosomal methylase (erm) genes: ermA and ermC. Among 432 CLDM-susceptible S. aureus isolates, 138 (31.9%) exhibited an iMLSB-resistance phenotype, with methicillinresistant S. aureus isolates (MRSA; 61 isolates: 58.6%) exhibiting higher positivity than methicillin-sensitive S. aureus isolates (MSSA; 77 isolates: 23.5%) (p<0.001). Male patients had a higher frequency of iMLSB resistance than females (OR [95%CI]: 1.8 [1.2-2.8]; p=0.007). Genotypically, ermA predominated in both MSSA (70.1%) and MRSA (86.9%) compared to ermC (14.3% in MSSA and 11.5% in MRSA). A single strain of MRSA possessed both ermA and ermC, while 12 (15.6%) MSSA isolates were negative for both ermA and ermC, suggesting the presence of other genetic mechanisms. Collectively, these results show that approximately 33% of CLDM-susceptible S. aureus isolates at our university hospital exhibited iMLSB resistance, predominantly caused by ermA in both MSSA and MRSA. |
| Keywords | antimicrobial resistance clindamycin erm D-zone test inducible MLSB |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2023-02 |
| Volume | volume77 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 9 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2023 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 36849140 |
| Web of Science KeyUT | 000953663800001 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Ojima, Hinako| Kuraoka, Sakiko| Okanoue, Shyoutarou| Okada, Hiroyuki| Gotoh, Kazuyoshi| Matsushita, Osamu| Watanabe, Akari| Yokota, Kenji| |
| Keywords | Helicobacter pylori nitrate-reducing bacteria IL-8 TNF-alpha cell cycle |
| Published Date | 2022-12-16 |
| Publication Title | Microorganisms |
| Volume | volume10 |
| Issue | issue12 |
| Publisher | MDPI |
| Start Page | 2495 |
| ISSN | 2076-2607 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 by the authors. |
| File Version | publisher |
| PubMed ID | 36557748 |
| DOI | 10.3390/microorganisms10122495 |
| Web of Science KeyUT | 000903731600001 |
| Related Url | isVersionOf https://doi.org/10.3390/microorganisms10122495 |
| FullText URL | fulltext20221116-2.pdf |
|---|---|
| Author | Gotoh, Kazuyoshi| Hagiya, Hideharu| Iio, Koji| Yamada, Haruto| Matsushita, Osamu| Otsuka, Fumio| |
| Keywords | Antimicrobial resistance Carbapenemase-producing Enterobacterales Carbapenem-resistant Enterobacterales New Delhi metallo-β-lactamase (NDM) Enterobacter cloacae complex |
| Note | © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.jiac.2022.08.019] .| This full-text will be available in Oct. 2023.| |
| Published Date | 2022-12 |
| Publication Title | Journal of Infection and Chemotherapy |
| Volume | volume28 |
| Issue | issue12 |
| Publisher | Elsevier BV |
| Start Page | 1697 |
| End Page | 1699 |
| ISSN | 1341-321X |
| NCID | AA11057978 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. |
| File Version | author |
| PubMed ID | 36049614 |
| DOI | 10.1016/j.jiac.2022.08.019 |
| Web of Science KeyUT | 000874559900019 |
| Related Url | isVersionOf https://doi.org/10.1016/j.jiac.2022.08.019 |
| FullText URL | fulltext20220830-2.pdf table20220830-2pdf.pdf Figs20220830-2.pdf Supplementary_data.pdf |
|---|---|
| Author | Uchiyama, Jumpei| Takemura-Uchiyama, Iyo| Gotoh, Kazuyoshi| Kato, Shin-ichiro| Sakaguchi, Yoshihiko| Murakami, Hironobu| Fukuyama, Tomoki| Kaneki, Mao| Matsushita, Osamu| Matsuzaki, Shigenobu| |
| Keywords | Environmental virus Jumbophage Metagenomics Evolution Uncultured phage |
| Note | © 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.virusres.2022.198881] . | |
| Published Date | 2022-10-02 |
| Publication Title | Virus Research |
| Volume | volume319 |
| Publisher | Elsevier BV |
| Start Page | 198881 |
| ISSN | 0168-1702 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 Elsevier B.V. |
| File Version | author |
| PubMed ID | 35934259 |
| DOI | 10.1016/j.virusres.2022.198881 |
| Web of Science KeyUT | 000841172200001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.virusres.2022.198881 |
| FullText URL | fulltext20211206-1.pdf |
|---|---|
| Author | Gotoh, Kazuyoshi| Miyoshi, Makoto| Mayura, I Putu Bayu| Iio, Koji| Matsushita, Osamu| Otsuka, Fumio| Hagiya, Hideharu| |
| Note | This is an Accepted Manuscript published by Microbiology Society.| |
| Published Date | 2021-10-4 |
| Publication Title | Journal of Medical Microbiology |
| Volume | volume70 |
| Issue | issue10 |
| Publisher | Microbiology Society |
| ISSN | 0022-2615 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 Microbiology Society |
| File Version | author |
| PubMed ID | 34605760 |
| DOI | 10.1099/jmm.0.001430 |
| Web of Science KeyUT | 000718022100014 |
| Related Url | isVersionOf https://doi.org/10.1099/jmm.0.001430 |
| FullText URL | fulltext20211109-5.pdf |
|---|---|
| Author | Gotoh, Kazuyoshi| Mayura, I. Putu Bayu| Enomoto, Yusaku| Iio, Koji| Matsushita, Osamu| Otsuka, Fumio| Hagiya, Hideharu| |
| Keywords | Antimicrobial Resistance Daptomycin resistance Corynebacterium striatum Insertion sequence pgsA2 gene |
| Note | This is an Accepted Manuscript of an article published by Springer. This fulltext is available in Oct. 2022.| |
| Published Date | 2021-10-21 |
| Publication Title | European Journal of Clinical Microbiology & Infectious Diseases |
| Volume | volume41 |
| Issue | issue2 |
| Publisher | Springer Science and Business Media LLC |
| Start Page | 331 |
| End Page | 333 |
| ISSN | 0934-9723 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature |
| File Version | author |
| PubMed ID | 34671843 |
| DOI | 10.1007/s10096-021-04369-1 |
| Web of Science KeyUT | 000709253000001 |
| Related Url | isVersionOf https://doi.org/10.1007/s10096-021-04369-1 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Ichihara, Aina| Ojima, Hinako| Gotoh, Kazuyoshi| Matsushita, Osamu| Take, Susumu| Okada, Hiroyuki| Watanabe, Akari| Yokota, Kenji| |
| Keywords | antibody VacA CagA genome |
| Published Date | 2021-07-05 |
| Publication Title | toxins |
| Volume | volume13 |
| Issue | issue7 |
| Publisher | MDPI |
| Start Page | 467 |
| ISSN | 2072-6651 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 by the authors. |
| File Version | publisher |
| DOI | 10.3390/toxins13070467 |
| Web of Science KeyUT | 000677052200001 |
| Related Url | isVersionOf https://doi.org/10.3390/toxins13070467 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Kobatake, Tomomi| Ogino, Keiki| Sakae, Hiroyuki| Gotoh, Kazuyoshi| Watanabe, Akari| Matsushita, Osamu| Okada, Hiroyuki| Yokota, Kenji| |
| Keywords | disulfiram Helicobacter pylori urease vacuolating toxin CagA |
| Published Date | 2021-05-12 |
| Publication Title | Infection and Drug Resistance |
| Volume | volume14 |
| Publisher | Dove Medical Press Ltd |
| Start Page | 1757 |
| End Page | 1764 |
| ISSN | 1178-6973 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2021 Kobatake et al. |
| File Version | publisher |
| PubMed ID | 34012274 |
| NAID | 120007042392 |
| DOI | 10.2147/IDR.S299177 |
| Web of Science KeyUT | 000653055700001 |
| Related Url | isVersionOf https://doi.org/10.2147/IDR.S299177 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nakamura, Shin| Ito, Takashi| Okamoto, Kentaro| Mima, Takehiko| Uchida, Kentaro| Siddiqui, Yasir D.| Ito, Masahiro| Tai, Masako| Okubo, Keisuke| Yamashiro, Keisuke| Omori, Kazuhiro| Yamamoto, Tadashi| Matsushita, Osamu| Takashiba, Shogo| |
| Keywords | bone regeneration collagen drug delivery systems growth factors periodontitis tissue engineering |
| Published Date | 2019-03-19 |
| Publication Title | Journal of Periodontology |
| Volume | volume90 |
| Issue | issue9 |
| Publisher | Wiley |
| Start Page | 1043 |
| End Page | 1052 |
| ISSN | 0022-3492 |
| NCID | AA00704417 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2019 The Authors |
| File Version | publisher |
| PubMed ID | 30889294 |
| DOI | 10.1002/JPER.18-0674 |
| Web of Science KeyUT | 000485288400012 |
| Related Url | isVersionOf https://doi.org/10.1002/JPER.18-0674 |
| JaLCDOI | 10.18926/AMO/49667 |
|---|---|
| FullText URL | 67_2_93.pdf |
| Author | Kita, Masahide| Yokota, Kenji| Okada, Hiroyuki| Take, Susumu| Takenaka, Ryuta| Kawahara, Yoshiro| Oguma, Keiji| Matsushita, Osamu| Yamamoto, Kazuhide| |
| Abstract | Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer and duodenal ulcer accompanied by atrophic gastritis in background mucosa. H. pylori strains were stored at －80℃ prior to the experiments being carried out. We analyzed iceA, babA, vacA, cagA, and cagE genes by PCR. The cagA gene was analyzed through sequencing of the C-terminal region containing the EPIYA motif, which is related to tyrosine phosphorylation. Severe atrophy was observed in patients with gastric ulcer. The major phenotype of the vacA gene was s1c/m1 (93ｵ). The cagA gene was detected in all strains. The cagE gene was not detected in 2 and 5 strains from the mild cases and severe cases, respectively. The major cagA EPIYA motif, which is amino acids repeat in the C terminus, was the A-B-D type (44 of 58 strains). The virulence genes were not statistically associated with the severity of atrophy in the background gastric mucosa in humans. Not only identification of bacterial virulence factors but also studies of the host response will be necessary to investigate the progression of gastric atrophy and subsequent cancer development in humans. |
| Keywords | Helicobacter pylori virulence genes chronic atrophic gastritis |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-04 |
| Volume | volume67 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 93 |
| End Page | 98 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23603925 |
| Web of Science KeyUT | 000317801700003 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52508 |
| JaLCDOI | 10.18926/AMO/49252 |
|---|---|
| FullText URL | 67_1_9.pdf |
| Author | Fatmawati, Ni Nengah Dwi| Sakaguchi, Yoshihiko| Suzuki, Tomonori| Oda, Masataka| Shimizu, Kenta| Yamamoto, Yumiko| Sakurai, Jun| Matsushita, Osamu| Oguma, Keiji| |
| Abstract | Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival. |
| Keywords | botulinum phospholipase C botulinum toxin phospholipase C activity sphingomyelinase activity hemolytic activity |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-02 |
| Volume | volume67 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 9 |
| End Page | 18 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23439504 |
| Web of Science KeyUT | 000316829900002 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/49731 |
