ID | 59907 |
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Author |
Nakamura, Shin
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Ito, Takashi
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Okamoto, Kentaro
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Mima, Takehiko
Department of Bacteriology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Uchida, Kentaro
Department of Orthopedic Surgery, Kitasato University School of Medicine
Siddiqui, Yasir D.
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Ito, Masahiro
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Tai, Masako
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Okubo, Keisuke
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Yamashiro, Keisuke
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
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Omori, Kazuhiro
Department of Periodontics and Endodontics, Okayama University Hospital
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Yamamoto, Tadashi
Department of Periodontics and Endodontics, Okayama University Hospital
Matsushita, Osamu
Department of Bacteriology, Dentistry and Pharmaceutical Sciences
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Takashiba, Shogo
Department of Pathophysiology‐Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
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Abstract | Background
Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen‐binding bFGF (CB‐bFGF) has been shown to enhance bone formation by collagen‐anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB‐bFGF with collagen scaffolds in bone regeneration of horizontal bone defect.
Methods
Cell proliferation activity and collagen binding activity of CB‐bFGF was confirmed by WST‐8 assay and collagen binding assay, respectively. The retention of CB‐bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB‐bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses.
Results
CB‐bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB‐bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB‐bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB‐bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin‐positive cells at the regeneration site in CB‐bFGF/CP group were greater than those in other groups.
Conclusions
CB‐bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB‐bFGF composite material for improved periodontal regeneration in vertical axis was shown.
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Keywords | bone regeneration
collagen
drug delivery systems
growth factors
periodontitis
tissue engineering
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Published Date | 2019-03-19
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Publication Title |
Journal of Periodontology
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Volume | volume90
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Issue | issue9
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Publisher | Wiley
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Start Page | 1043
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End Page | 1052
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ISSN | 0022-3492
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NCID | AA00704417
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2019 The Authors
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1002/JPER.18-0674
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Agency for Medical Research and Development
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | H27-052
H28-111
18K07111
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