Title Alternative イネ幼植物から調整した細胞壁に含まれるペクチン質の性状
FullText URL 005_002_135_144.pdf
Author Konno, Haruyoshi| Tsumuki, Hisaaki| Yamasaki, Yoshiki|
Abstract Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4~0.6% of the starch-free cell walls.
Keywords Cell wall Oryza sativa Pectic polymer
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 135
End Page 144
ISSN 0916-930X
language English
File Version publisher
Title Alternative Purification and Properties of α-Glucodidase from Taro Tuber
FullText URL 005_002_129_134.pdf
Author Mashima, Hideyuki| Yamasaki, Yoshiki| Konno, Haruyoshi|
Abstract α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose.
Keywords α-glucosidase taro tuber Colocasia esculanta Shott
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 129
End Page 134
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative ナメクジのα-グルコシダーゼの精製と性質
FullText URL 005_002_121_127.pdf
Author Yamasaki, Yoshiki| Konno, Haruyoshi|
Abstract Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly.
Keywords Slug Incilaria bilineata α-glucosidase
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 121
End Page 127
ISSN 0916-930X
language English
File Version publisher
Title Alternative テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質
FullText URL 001_002_159_166.pdf
Author Yamasaki, Yoshiki| Konno, Haruyoshi|
Abstract Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity.
Keywords Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast
Publication Title Bulletin of the Research Institute For Bioresources, Okayama University
Published Date 1993
Volume volume1
Issue issue2
Start Page 159
End Page 166
ISSN 0916-930X
language English
File Version publisher
Title Alternative ニンジン懸濁培養細胞の培養液に分泌される細胞外多糖成分
FullText URL 001_002_091_103.pdf
Author Konno, Haruyoshi| Yamasaki, Yoshiki| Katoh, Kenji|
Abstract The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized.
Keywords Carrot Cell suspension culture Cell wall Extracellular polysaccharides Pectic polymer
Publication Title Bulletin of the Research Institute For Bioresources, Okayama University
Published Date 1993
Volume volume1
Issue issue2
Start Page 91
End Page 103
ISSN 0916-930X
language English
File Version publisher