| Title Alternative | ニンジン懸濁培養細胞の培養液に分泌される細胞外多糖成分 |
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| FullText URL | 001_002_091_103.pdf |
| Author | Konno, Haruyoshi| Yamasaki, Yoshiki| Katoh, Kenji| |
| Abstract | The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized. |
| Keywords | Carrot Cell suspension culture Cell wall Extracellular polysaccharides Pectic polymer |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1993 |
| Volume | volume1 |
| Issue | issue2 |
| Start Page | 91 |
| End Page | 103 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
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| FullText URL | 001_002_159_166.pdf |
| Author | Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| Keywords | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| Publication Title | Bulletin of the Research Institute For Bioresources, Okayama University |
| Published Date | 1993 |
| Volume | volume1 |
| Issue | issue2 |
| Start Page | 159 |
| End Page | 166 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | ナメクジのα-グルコシダーゼの精製と性質 |
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| FullText URL | 005_002_121_127.pdf |
| Author | Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| Keywords | Slug Incilaria bilineata α-glucosidase |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 121 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Purification and Properties of α-Glucodidase from Taro Tuber |
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| FullText URL | 005_002_129_134.pdf |
| Author | Mashima, Hideyuki| Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. |
| Keywords | α-glucosidase taro tuber Colocasia esculanta Shott |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 129 |
| End Page | 134 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
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| FullText URL | 005_002_135_144.pdf |
| Author | Konno, Haruyoshi| Tsumuki, Hisaaki| Yamasaki, Yoshiki| |
| Abstract | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| Keywords | Cell wall Oryza sativa Pectic polymer |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 135 |
| End Page | 144 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
