Hirohata, Satoshi - OU Author List - OKAYAMA UNIVERSITY SCIENTIFIC ACHIEVEMENT REPOSITORY

The 3'-untranslated region of ADAMTS1 regulates its mRNA stability

JaLCDOI	10.18926/AMO/31831
FullText URL	fulltext.pdf
Author	Hatipoglu, Omer Faruk\| Hirohata, Satoshi\| Yaykasli, Kursat Oguz\| Cilek, Mehmet Zeynel\| Demircan, Kadir\| Shinohata, Ryoko\| Yonezawa, Tomoko\| Oohashi, Toshitaka\| Kusachi, Shozo\| Ninomiya, Yoshifumi\|
Abstract	ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.
Keywords	ADAMTS1 gene regulation metalloproteinase
Amo Type	Original Article
Publication Title	Acta Medica Okayama
Published Date	2009-04
Volume	volume63
Issue	issue2
Publisher	Okayama University Medical School
Start Page	79
End Page	85
ISSN	0386-300X
NCID	AA00508441
Content Type	Journal Article
language	English
File Version	publisher
Refereed	True
PubMed ID	19404339
Web of Science KeyUT	000265457600002

Eosinophil Cationic Protein Shows Survival Effect on H9c2 Cardiac Myoblast Cells with Enhanced Phosphorylation of ERK and Akt/GSK-3β under Oxidative Stress

JaLCDOI	10.18926/AMO/53521
FullText URL	69_3_145.pdf
Author	Ishii, Hiroko\| Kamikawa, Shigeshi\| Hirohata, Satoshi\| Mizutani, Akifumi\| Abe, Koji\| Seno, Masaharu\| Oohashi, Toshitaka\| Ninomiya, Yoshifumi\|
Abstract	Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.
Keywords	ECP reactive oxygen species Akt ERK
Amo Type	Original Article
Publication Title	Acta Medica Okayama
Published Date	2015-06
Volume	volume69
Issue	issue3
Publisher	Okayama University Medical School
Start Page	145
End Page	153
ISSN	0386-300X
NCID	AA00508441
Content Type	Journal Article
language	English
Copyright Holders	CopyrightⒸ 2015 by Okayama University Medical School
File Version	publisher
Refereed	True
PubMed ID	26101190
Web of Science KeyUT	000356903000003

Increased Glycine-conjugated and Unconjugated Bile Acid Levels Associated with Aggravation of Nonalcoholic Steatohepatitis and Cardiovascular Disease in SHRSP5/Dmcr Rat

JaLCDOI	10.18926/AMO/64358
FullText URL	77_1_29.pdf
Author	Yamamoto, Shusei\| Sato, Ikumi\| Fujii, Moe\| Kakimoto, Mai\| Honma, Koki\| Akiyama, Natsumi\| Sakai, Miku\| Fukuhama, Natsuki\| Kumazaki, Shota\| Hirohata, Satoshi\| Kitamori, Kazuya\| Yamori, Yukio\| Watanabe, Shogo\|
Abstract	The SHRSP5/Dmcr is a useful animal model for the development of nonalcoholic steatohepatitis (NASH) pathology when fed a high-fat, high-cholesterol diet, and further drug interventions can lead to concomitant cardiovascular disease. While SHRSP5/Dmcr rats have been used for basic research related to NASH, details of their bile acid metabolism in this condition are unknown. In this study, we aimed to clarify the changes in the serum bile acid (BA) fractions associated with NASH and found that glycine-conjugated and unconjugated bile acid increased with worsening NASH and cardiovascular disease while taurine-conjugated BA relatively decreased.
Keywords	SHRSP5/Dmc nonalcoholic steatohepatitis cardiovascular disease glycine-conjugated bile acids unconjugated bile acids
Amo Type	Original Article
Publication Title	Acta Medica Okayama
Published Date	2023-02
Volume	volume77
Issue	issue1
Publisher	Okayama University Medical School
Start Page	29
End Page	36
ISSN	0386-300X
NCID	AA00508441
Content Type	Journal Article
language	English
Copyright Holders	Copyright Ⓒ 2023 by Okayama University Medical School
File Version	publisher
Refereed	True
PubMed ID	36849143
Web of Science KeyUT	000953019200001

Laminin α1,α'2,α4 and β1,Chain mRNA Expression in Mouse Embryonic,Neonatal, and Adult Hearts

Author	廣畑聡\|
Published Date	1997-03-25
Publication Title
Content Type	Thesis or Dissertation

ADAMTS-9 is synergistically induced by interleukin-1 and tumor necrosis factor in OUMS-27 chondrosarcoma cells and in human chondrocytes

Author	Demircan, Kadir\| Hirohata, Satoshi\| Nishida, Keiichiro\| Hatipoglu, Omer F.\| Oohashi, Toshitaka\| Yonezawa, Tomoko\| Apte, Suneel S.\| Ninomiya, Yoshifumi\|
Published Date	2005-5
Publication Title	Arthritis & Rheumatism
Volume	volume52
Issue	issue5
Content Type	Journal Article

Versican is induced in infiltrating monocytes in myocardial infarction

Author	Toeda, Kenichi\| Nakamura, Keigo\| Hirohata, Satoshi\| Hatipoglu, Omer F.\| Demircan, Kadir\| Yamawaki, Hitoshi\| Ogawa, Hiroko\| Kusachi, Shozo\| Shiratori, Yasushi\| Ninomiya, Yoshifumi\|
Published Date	2005-12
Publication Title	Molecular and Cellular Biochemistry
Volume	volume280
Issue	issue1-2
Content Type	Journal Article

Mechanical strain attenuates cytokine-induced ADAMTS9 expression via transient receptor potential vanilloid type 1

FullText URL	fulltext.pdf
Author	Ohtsuki, Takashi\| Shinaoka, Akira\| Kumagishi-Shinaoka, Kanae\| Asano, Keiichi\| Hatipoglu, Omer Faruk\| Inagaki, Junko\| Takahashi, Ken\| Oohashi, Toshitaka\| Nishida, Keiichiro\| Naruse, Keiji\| Hirohata, Satoshi\|
Published Date	2019-10-15
Publication Title	Experimental Cell Research
Volume	volume383
Issue	issue2
Publisher	Elsevier
Start Page	111556
ISSN	00144827
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2019 The Authors. Published by Elsevier Inc.
File Version	publisher
PubMed ID	31415758
DOI	10.1016/j.yexcr.2019.111556
Web of Science KeyUT	000488145400017
Related Url	isVersionOf https://doi.org/10.1016/j.yexcr.2019.111556

Induction of CEMIP in Chondrocytes by Inflammatory Cytokines: Underlying Mechanisms and Potential Involvement in Osteoarthritis

FullText URL	fulltext.pdf
Author	Ohtsuki, Takashi\| Hatipoglu, Omer F.\| Asano, Keiichi\| Inagaki, Junko\| Nishida, Keiichiro\| Hirohata, Satoshi\|
Keywords	cell migration-inducing hyaluronidase 1 (CEMIP) chondrocyte hyaluronan mechanical strain nuclear factor kappa B (NF-kappa B) osteoarthritis
Published Date	2020-04-29
Publication Title	International Journal of Molecular Sciences
Volume	volume21
Issue	issue9
Publisher	MDPI
Start Page	3140
ISSN	1422-0067
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2020 by the authors.
File Version	publisher
PubMed ID	32365591
DOI	10.3390/ijms21093140
Web of Science KeyUT	000535581700111
Related Url	isVersionOf https://doi.org/10.3390/ijms21093140

Deficiency of CD44 prevents thoracic aortic dissection in a murine model

FullText URL	fulltext.pdf
Author	Hatipoglu, Omer F.\| Miyoshi, Toru\| Yonezawa, Tomoko\| Kondo, Megumi\| Amioka, Naofumi\| Yoshida, Masashi\| Akagi, Satoshi\| Nakamura, Kazufumi\| Hirohata, Satoshi\| Ito, Hiroshi\|
Keywords	Aneurysm Aortic diseases
Published Date	2020-04-22
Publication Title	Scientific Reports
Volume	volume10
Issue	issue1
Publisher	Nature
Start Page	6869
ISSN	2045-2322
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© The Author(s) 2020
File Version	publisher
PubMed ID	32321956
DOI	10.1038/s41598-020-63824-9
Web of Science KeyUT	000560329100004
Related Url	isVersionOf https://doi.org/10.1038/s41598-020-63824-9

Osteopontin silencing attenuates bleomycin-induced murine pulmonary fibrosis by regulating epithelial-mesenchymal transition

FullText URL	fulltext.pdf
Author	Hatipoglu, Omer Faruk\| Uctepe, Eyyup\| Opoku, Gabriel\| Wake, Hidenori\| Ikemura, Kentaro\| Ohtsuki, Takashi\| Inagaki, Junko\| Gunduz, Mehmet\| Gunduz, Esra\| Watanabe, Shogo\| Nishinaka, Takashi\| Takahashi, Hideo\| Hirohata, Satoshi\|
Keywords	Pulmonary fibrosis Osteopontin Epithelial-mesenchymal transition
Published Date	2021-07
Publication Title	Biomedicine & Pharmacotherapy
Volume	volume139
Publisher	Elsevier France-Editions Scientifiques Medicales e
Start Page	111633
ISSN	0753-3322
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2021 The Author(s).
File Version	publisher
DOI	10.1016/j.biopha.2021.111633
Web of Science KeyUT	000663683300007
Related Url	isVersionOf https://doi.org/10.1016/j.biopha.2021.111633

Potential of a Novel Chemical Compound Targeting Matrix Metalloprotease-13 for Early Osteoarthritis: An In Vitro Study

FullText URL	fulltext.pdf
Author	Inagaki, Junko\| Nakano, Airi\| Hatipoglu, Omer Faruk\| Ooka, Yuka\| Tani, Yurina\| Miki, Akane\| Ikemura, Kentaro\| Opoku, Gabriel\| Ando, Ryosuke\| Kodama, Shintaro\| Ohtsuki, Takashi\| Yamaji, Hirosuke\| Yamamoto, Shusei\| Katsuyama, Eri\| Watanabe, Shogo\| Hirohata, Satoshi\|
Keywords	osteoarthritis matrix metalloproteinase MMP13 ADAMTS9 expression screening chondrocytes
Published Date	2022-02-28
Publication Title	International Journal Of Molecular Sciences
Volume	volume23
Issue	issue5
Publisher	MDPI
Start Page	2681
ISSN	1422-0067
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2022 by the authors.
File Version	publisher
PubMed ID	35269821
DOI	10.3390/ijms23052681
Web of Science KeyUT	000773522200001
Related Url	isVersionOf https://doi.org/10.3390/ijms23052681

Evidence for Hypoxia-Induced Shift in ATP Production from Glycolysis to Mitochondrial Respiration in Pulmonary Artery Smooth Muscle Cells in Pulmonary Arterial Hypertension

FullText URL	fulltext.pdf
Author	Akagi, Satoshi\| Nakamura, Kazufumi\| Kondo, Megumi\| Hirohata, Satoshi\| Udono, Heiichiro\| Nishida, Mikako\| Saito, Yukihiro\| Yoshida, Masashi\| Miyoshi, Toru\| Ito, Hiroshi\|
Keywords	glycolysis mitochondrial respiration pulmonary arterial hypertension pulmonary artery smooth muscle cells Seahorse technology hypoxia ATP production
Published Date	2023-07-31
Publication Title	Journal of Clinical Medicine
Volume	volume12
Issue	issue15
Publisher	MDPI
Start Page	5028
ISSN	2077-0383
Content Type	Journal Article
language	English
OAI-PMH Set	岡山大学
Copyright Holders	© 2023 by the authors.
File Version	publisher
PubMed ID	37568430
DOI	10.3390/jcm12155028
Web of Science KeyUT	001045690800001
Related Url	isVersionOf https://doi.org/10.3390/jcm12155028