FullText URL fulltext.pdf
Author Kono, Reika| Hamasaki, Ichiro| Kishimoto, Fumiko| Shimizu, Takehiro| Kindo, Hiroya| Shibata, Kiyo| Morisawa, Shin| Morizane, Yuki|
Keywords High myopia Strabismus Eye dimensions Magnetic resonance imaging Esotropia
Note The version of record of this article, first published in Japanese Journal of Ophthalmology, is available online at Publisher’s website: http://dx.doi.org/10.1007/s10384-024-01133-8|
Published Date 2024-11-08
Publication Title Japanese Journal of Ophthalmology
Volume volume68
Issue issue6
Publisher Springer Science and Business Media LLC
Start Page 641
End Page 650
ISSN 0021-5155
NCID AA00691177
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2024
File Version publisher
PubMed ID 39514039
DOI 10.1007/s10384-024-01133-8
Web of Science KeyUT 001351168700003
Related Url isVersionOf https://doi.org/10.1007/s10384-024-01133-8
FullText URL fulltext.pdf
Author Matoba, Ryo| Morizane, Yuki|
Keywords En face imaging Epiretinal membrane Epiretinal proliferation Internal limiting membrane Lamellar macular hole
Note The version of record of this article, first published in Japanese Journal of Ophthalmology, is available online at Publisher’s website: http://dx.doi.org/10.1007/s10384-024-01127-6|
Published Date 2024-10-28
Publication Title Japanese Journal of Ophthalmology
Volume volume68
Issue issue6
Publisher Springer Science and Business Media LLC
Start Page 603
End Page 613
ISSN 0021-5155
NCID AA00691177
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2024
File Version publisher
PubMed ID 39466371
DOI 10.1007/s10384-024-01127-6
Web of Science KeyUT 001344343900001
Related Url isVersionOf https://doi.org/10.1007/s10384-024-01127-6
Author Matoba, Ryo| Kanzaki, Yuki| Morita, Tetsuro| Masuda, Yuki| Kimura, Shuhei| Hosokawa, Mio M.| Shiode, Yusuke| Morizane, Yuki|
Keywords en-face imaging epiretinal membrane epiretinal membrane foveoschisis foveoschisis lamellar macular hole metamorphopsia optical coherence tomography retinal fold retinal traction
Note This is a non-final version of an article published in final form in Matoba, Ryo MD, PhD; Kanzaki, Yuki MD, PhD; Morita, Tetsuro MD; Masuda, Yuki MD; Kimura, Shuhei MD, PhD; Hosokawa, Mio M. MD, PhD; Shiode, Yusuke MD, PhD; Morizane, Yuki MD, PhD. INVESTIGATION OF THE PATHOPHYSIOLOGY OF EPIRETINAL MEMBRANE FOVEOSCHISIS: Analysis of Longitudinal Changes in Visual Functions, Retinal Structures, and Retinal Traction Force. Retina 44(11):p 1992-2000, November 2024. | DOI: 10.1097/IAE.0000000000004217."| This fulltext file will be available in Nov. 2025.|
Published Date 2024-11
Publication Title Retina
Volume volume44
Issue issue11
Publisher Ovid Technologies (Wolters Kluwer Health)
Start Page 1992
End Page 2000
ISSN 0275-004X
NCID AA10639923
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2024, Wolters Kluwer Health
File Version author
PubMed ID 39024655
DOI 10.1097/iae.0000000000004217
Related Url isVersionOf https://doi.org/10.1097/iae.0000000000004217
Author Matoba, Ryo| Kanzaki, Yuki| Morita, Tetsuro| Masuda, Yuki| Kimura, Shuhei| Hosokawa, Mio M.| Shiode, Yusuke| Morizane, Yuki|
Keywords en-face imaging epiretinal membrane epiretinal membrane foveoschisis lamellar macular hole metamorphopsia mixed type optical coherence tomography retinal fold retinal traction
Note This is a non-final version of an article published in final form in Matoba, Ryo MD, PhD; Kanzaki, Yuki MD, PhD; Morita, Tetsuro MD; Masuda, Yuki MD; Kimura, Shuhei MD, PhD; Hosokawa, Mio M. MD, PhD; Shiode, Yusuke MD, PhD; Morizane, Yuki MD, PhD. MIXED PATHOPHYSIOLOGIES OF LAMELLAR MACULAR HOLES AND RELATED DISEASES: A Multimodal Optical Coherence Tomography–Based Study. Retina 44(10):p 1785-1792, October 2024. | DOI: 10.1097/IAE.0000000000004166.| This fulltext file will be available in Oct. 2025.|
Published Date 2024-10
Publication Title Retina
Volume volume44
Issue issue10
Publisher Ovid Technologies (Wolters Kluwer Health)
Start Page 1785
End Page 1792
ISSN 0275-004X
NCID AA10639923
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2024, Wolters Kluwer Health
File Version author
PubMed ID 39287541
DOI 10.1097/iae.0000000000004166
Web of Science KeyUT 001334163300005
Related Url isVersionOf https://doi.org/10.1097/iae.0000000000004166
JaLCDOI 10.18926/AMO/67206
FullText URL 78_3_295.pdf
Author Okamoto, Sara| Shiode, Yusuke| Kimura, Shuhei| Hosokawa, Mio| Matoba, Ryo| Kanzaki, Yuki| Kindo, Hiroya| Morita, Tetsuro| Tsuji, Akihiro| Takahashi, Kosuke| Morizane, Yuki|
Abstract A Japanese woman in her 70s was referred to our hospital for the evaluation and treatment of high intraocular pressure (IOP) in her right eye. She had undergone bilateral cataract surgeries and the insertion of hydrophilic acrylic intraocular lenses (IOLs). We performed trabeculotomy and trabeculectomy to lower her right IOP; thereafter, a circular opacity was observed on the right eye’s IOL surface. We removed the right IOL because that eye’s vision had decreased due to IOL opacification. The analysis of the removed IOL revealed that the main opacity component was calcium phosphate. This is the first post-glaucoma-surgery IOL calcification case report.
Keywords intraocular lens IOL IOL calcification hydrophilic acrylic IOL glaucoma surgery
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2024-06
Volume volume78
Issue issue3
Publisher Okayama University Medical School
Start Page 295
End Page 300
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders Copyright Ⓒ 2024 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 38902219
Web of Science KeyUT 001267339400001
FullText URL fulltext.pdf
Author Kanzaki, Yuki| Matoba, Ryo| Ishihara, Kenji| Morita, Tetsuro| Muraoka, Yuki| Kimura, Shuhei| Koto, Takashi| Kawasaki, Ryo| Baba, Takayuki| Okamoto, Fumiki| Inoue, Makoto| Sakamoto, Taiji| Tsujikawa, Akitaka| Morizane, Yuki|
Published Date 2024-02-08
Publication Title PLoS ONE
Volume volume19
Issue issue2
Publisher Public Library of Science
Start Page e0297347
ISSN 1932-6203
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2024 Kanzaki et al.
File Version publisher
PubMed ID 38329968
DOI 10.1371/journal.pone.0297347
Web of Science KeyUT 001164302700019
Related Url isVersionOf https://doi.org/10.1371/journal.pone.0297347
FullText URL fulltext.pdf
Author Kanzaki, Yuki| Matoba, Ryo| Kimura, Shuhei| Hosokawa, Mio M.| Shiode, Yusuke| Doi, Shinichiro| Morita, Tetsuro| Kanzaki, Sayumi| Takasu, Ippei| Tanikawa, Atsuhiro| Morizane, Yuki|
Keywords Epiretinal membrane En face OCT Retinal traction force Inner nuclear layer Focal macular electroretinogram
Published Date 2023-09
Publication Title Ophthalmology Science
Volume volume3
Issue issue3
Publisher Elsevier
Start Page 100312
ISSN 2666-9145
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2023 by the American Academy of Ophthalmology.
File Version publisher
PubMed ID 37214764
DOI 10.1016/j.xops.2023.100312
Web of Science KeyUT 000998320200001
Related Url isVersionOf https://doi.org/10.1016/j.xops.2023.100312
FullText URL fulltext.pdf
Author Mino, Mai| Matoba, Ryo| Kanzaki, Yuki| Kimura, Shuhei| Hosokawa, Mio M.| Shiode, Yusuke| Morita, Tetsuro| Morizane, Yuki|
Keywords Epiretinal membrane foveoschisis Lamellar macular hole Macular pseudohole Metamorphopsia Optical coherence tomography
Published Date 2023-09
Publication Title Ophthalmology Science
Volume volume3
Issue issue3
Publisher Elsevier
Start Page 100305
ISSN 2666-9145
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2023 by the American Academy of Ophthalmology.
File Version publisher
PubMed ID 37214763
DOI 10.1016/j.xops.2023.100305
Web of Science KeyUT 001033974700001
Related Url isVersionOf https://doi.org/10.1016/j.xops.2023.100305
FullText URL fulltext20231114-03.pdf
Author Matoba, Ryo| Morimoto, Noriko| Kawasaki, Ryo| Fujiwara, Miyuki| Kanenaga, Keisuke| Yamashita, Hidetoshi| Sakamoto, Taiji| Morizane, Yuki|
Keywords Visual impairment Japan Certification criteria Survey Glaucoma
Note This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10384-023-00986-9| This fulltext file will be available in Apr. 2024.|
Published Date 2023-04-17
Publication Title Japanese Journal of Ophthalmology
Volume volume67
Issue issue3
Publisher Springer Science and Business Media LLC
Start Page 346
End Page 352
ISSN 0021-5155
NCID AA00691177
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © Japanese Ophthalmological Society 2023
File Version author
PubMed ID 37067634
DOI 10.1007/s10384-023-00986-9
Web of Science KeyUT 000970743300001
Related Url isVersionOf https://doi.org/10.1007/s10384-023-00986-9
FullText URL fulltext20231114-02.pdf
Author Matoba, Ryo| Kanzaki, Yuki| Doi, Shinichiro| Kanzaki, Sayumi| Kimura, Shuhei| Hosokawa, Mio Morizane| Shiode, Yusuke| Takahashi, Kosuke| Morizane, Yuki|
Keywords Rhegmatogenous retinal detachment Epiretinal membrane Vitrectomy Internal limiting membrane En face optical coherence tomography Retinal folds
Note This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00417-021-05118-y|
Published Date 2021-03-12
Publication Title Graefe's Archive for Clinical and Experimental Ophthalmology
Volume volume259
Issue issue9
Publisher Springer Science and Business Media LLC
Start Page 2503
End Page 2512
ISSN 0721-832X
NCID AA10623581
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
File Version author
PubMed ID 33710473
DOI 10.1007/s00417-021-05118-y
Web of Science KeyUT 000628181800006
Related Url isVersionOf https://doi.org/10.1007/s00417-021-05118-y
FullText URL fulltext20231114-01.pdf
Author Matoba, Ryo| Kanzaki, Yuki| Morita, Tetsuro| Kimura, Shuhei| Hosokawa, Mio Morizane| Shiode, Yusuke| Morizane, Yuki|
Keywords Epiretinal membrane Scleral buckling Retinal detachment Optical coherence tomography En face imaging Posterior vitreous detachment
Note This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00417-023-06285-w| This fulltext file will be available in Oct. 2024.|
Published Date 2023-10-21
Publication Title Graefe's Archive for Clinical and Experimental Ophthalmology
Volume volume262
Issue issue2
Publisher Springer Science and Business Media LLC
Start Page 469
End Page 476
ISSN 0721-832X
NCID AA10623581
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023
File Version author
PubMed ID 37864637
DOI 10.1007/s00417-023-06285-w
Web of Science KeyUT 001089648100003
Related Url isVersionOf https://doi.org/10.1007/s00417-023-06285-w
JaLCDOI 10.18926/AMO/65756
FullText URL 77_4_433.pdf
Author Ono, Ryuki| Shiode, Yusuke| Kimura, Shuhei| Hosokawa, Mio| Matoba, Ryo| Kanzaki, Yuki| Kindo, Hiroya| Morita, Tetsuro| Morizane, Yuki|
Abstract A 23-year-old woman presented with a 1-month history of visual abnormalities in her right eye. A visual field test revealed temporal abnormalities in the right eye. Optical coherence tomography revealed an indistinct ellipsoid zone (EZ) on the B-scan image and hyporeflective areas in the EZ layer on the en face image in the right eye. We diagnosed the patient with acute zonal occult outer retinopathy. Visual field tests and B-scan images improved to almost normal at 6 months, but hyporeflective areas remained on the en face images. Thus, en face images may be more sensitive at detecting abnormalities in the outer retina than other modalities.
Keywords acute zonal occult outer retinopathy optical coherence tomography en face image ellipsoid zone
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2023-08
Volume volume77
Issue issue4
Publisher Okayama University Medical School
Start Page 433
End Page 437
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders Copyright Ⓒ 2023 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 37635145
Web of Science KeyUT 001088434900001
FullText URL fulltext20230821-02.pdf fig1_20230821-02.pdf fig2_20230821-02.pdf fig3_20230821-02.pdf fig4_20230821-02.pdf fig5_20230821-02.pdf fig6_20230821-02.pdf
Author Kimura, Shuhei| Hosokawa, Mio Morizane| Shiode, Yusuke| Matoba, Ryo| Kanzaki, Yuki| Goto, Yasuhito| Kanenaga, Keisuke| Suzuki, Etsuji| Morizane, Yuki|
Keywords Axial length Rhegmatogenous retinal detachment Macular detachment Fourier-domain optic biometry Ultrasound
Note This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10384-023-01018-2| This fulltext file will be available in Aug. 2024.|
Published Date 2023-08-10
Publication Title Japanese Journal of Ophthalmology
Volume volume67
Issue issue6
Publisher Springer Science and Business Media LLC
Start Page 645
End Page 651
ISSN 0021-5155
NCID AA00691177
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © Japanese Ophthalmological Society 2023
File Version author
PubMed ID 37561309
DOI 10.1007/s10384-023-01018-2
Web of Science KeyUT 001045669100002
Related Url isVersionOf https://doi.org/10.1007/s10384-023-01018-2
FullText URL fulltext20220428-6.pdf
Author Kanzaki, Yuki| Doi, Shinichiro| Matoba, Ryo| Kanzaki, Sayumi| Kimura, Shuhei| Hosokawa, Mio M.| Shiode, Yusuke| Takasu, Ippei| Morizane, Yuki|
Keywords en-face optical coherence tomography epiretinal membrane maximum depth of the retinal folds M-CHARTS metamorphopsia
Note This is a non-final version of an article published in final form in Kanzaki, Yuki MD, PhD*; Doi, Shinichiro MD, PhD*; Matoba, Ryo MD, PhD*; Kanzaki, Sayumi MD*; Kimura, Shuhei MD, PhD*; Hosokawa, Mio M. MD, PhD*; Shiode, Yusuke MD, PhD*; Takasu, Ippei MD, PhD†; Morizane, Yuki MD, PhD*. OBJECTIVE AND QUANTITATIVE ESTIMATION OF THE OPTIMAL TIMING FOR EPIRETINAL MEMBRANE SURGERY ON THE BASIS OF METAMORPHOPSIA. Retina 42(4):p 704-711, April 2022. | DOI: 10.1097/IAE.0000000000003401 .| This fulltext is available in Apr. 2023.|
Published Date 2022-04-01
Publication Title RETINA: The Journal of Retinal and Vitreous Diseases
Volume volume42
Issue issue4
Publisher Lippincott, Williams & Wilkins
Start Page 704
End Page 711
ISSN 0275-004X
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders Copyright © 2022, Wolters Kluwer Health
File Version author
PubMed ID 34983900
DOI 10.1097/iae.0000000000003401
Web of Science KeyUT 000774215200015
Related Url isVersionOf https://doi.org/10.1097/iae.0000000000003401
Title Alternative Artificial intelligence in ophthalmology
FullText URL 133_113.pdf
Author Morizane, Yuki|
Keywords 眼科 人工知能 AI 糖尿病網膜症 加齢黄斑変性
Publication Title Journal of Okayama Medical Association
Published Date 2021-08-02
Volume volume133
Issue issue2
Start Page 113
End Page 115
ISSN 0030-1558
Related Url isVersionOf https://doi.org/10.4044/joma.133.113
language Japanese
Copyright Holders Copyright (c) 2021 岡山医学会
File Version publisher
DOI 10.4044/joma.133.113
JaLCDOI 10.18926/AMO/62396
FullText URL 75_4_447.pdf
Author Kono, Reika| Hamasaki, Ichiro| Kishimoto, Fumiko| Ohtuski, Hiroshi| Shibata, Kiyo| Morizane, Yuki| Shiraga, Fumio|
Abstract In high myopia, eye dislocation due to increased globe volume or tight orbital volume causes acquired esotro-pia. GOR (globe/orbit volume ratio), an indicator of the degree of progression of this pathology, was investi-gated the relationships among easily obtained clinical parameters. In this retrospective study, 20 eyes from 10 acquired esotropia patients with high myopia but without abduction limitations were examined. The mean age of the patients was 63.7 ± 8.2 years (mean ± standard deviation). Volumes were measured on the three-dimen-sional fast imaging employing steady-state acquisition magnetic resonance imaging images using the vol-ume-measurement function. Correlations between GOR and the displacement angle of the globe (DA), axial length (AL), and equatorial diameter (ED) were investigated. Mean DA, AL, ED, and GOR values were 107.5 ± 8.5°, 28.86 ± 1.92 mm, 25.00 ± 1.16 mm, and 0.36 ± 0.05, respectively. Only AL was correlated with GOR (p < 0.0001, R2 = 0.6649); DA (p = 0.30, R2 = 0.0633) and ED (p = 0.91, R2 = 0.0008) were not. AL was the only clinically available parameter to indicate globe/orbit volume imbalances in acquired esotropia with high myopia but without abduction limitation. AL may be important for the clinical assessment of the progression of this pathology.
Keywords acquired esotropia high myopia (high myopes) globe volume magnetic resonance imaging limitation of abduction
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2021-08
Volume volume75
Issue issue4
Publisher Okayama University Medical School
Start Page 447
End Page 453
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2021 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 34511611
Web of Science KeyUT 000697944600006
NAID 120007146039
JaLCDOI 10.18926/AMO/62378
FullText URL 75_4_403.pdf
Author Matoba, Ryo| Morizane, Yuki|
Abstract Epiretinal membrane (ERM) is a common retinal disease characterized by cellular proliferation and metaplasia that lead to the formation of a pathological fibrocellular membrane immediately superjacent to the inner retinal surface. The vast majority of ERMs are considered idiopathic. However, ERM formation can result from various primary intraocular diseases, including retinal breaks and detachment, retinal vascular diseases, and vitreoretinal inflammatory conditions. Although ERMs are generally asymptomatic or cause mild metamorphopsia and/or a modest decrease in visual acuity, some can cause severe macular distortion and macular edema, resulting in significantly impaired function. Surgical removal of ERM is the only treatment, and improvements in vitrectomy systems have enabled less invasive treatment. However, there are currently no standardized criteria for ERM surgery, and the indications for surgery are determined from the patient’s subjective symptoms. Another problem with ERM surgery is that not all patients show satisfactory postoperative recovery of visual function. Thus, further research is needed to determine the criteria for ERM surgery and methods to improve the postoperative prognosis.
Keywords epiretinal membrane vitrectomy optical coherence tomography internal limiting membrane lamellar macular hole
Amo Type Review
Publication Title Acta Medica Okayama
Published Date 2021-08
Volume volume75
Issue issue4
Publisher Okayama University Medical School
Start Page 403
End Page 413
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2021 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 34511606
Web of Science KeyUT 000697944600001
NAID 120007146059
Title Alternative Advances in vitrectomy for diabetic retinopathy
FullText URL 133_36.pdf
Author Morizane, Yuki|
Keywords 糖尿病 糖尿病網膜症 糖尿病黄斑浮腫 硝子体手術
Publication Title Journal of Okayama Medical Association
Published Date 2021-04-01
Volume volume133
Issue issue1
Start Page 36
End Page 42
ISSN 0030-1558
Related Url isVersionOf https://doi.org/10.4044/joma.133.36
language Japanese
Copyright Holders Copyright (c) 2021 岡山医学会
File Version publisher
DOI 10.4044/joma.133.36
NAID 130008034815
FullText URL fulltext.pdf
Author Fujiwara, Atsushi| Kanzaki, Yuki| Kimura, Shuhei| Hosokawa, Mio| Shiode, Yusuke| Doi, Shinichiro| Takahashi, Kosuke| Matoba, Ryo| Morizane, Yuki|
Published Date 2021-04-07
Publication Title Scientific Reports
Volume volume11
Issue issue1
Publisher Nature Research
ISSN 2045-2322
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2021
File Version publisher
PubMed ID 33828222
NAID 120007037374
DOI 10.1038/s41598-021-87440-3
Web of Science KeyUT 000640395800029
Related Url isVersionOf https://doi.org/10.1038/s41598-021-87440-3
FullText URL fulltext.pdf
Author Noda, Hiroshi| Kimura, Shuhei| Hosokawa Morizane, Mio| Shiode, Yusuke| Doi, Shinichiro| Takahashi, Kosuke| Matoba, Ryo| Kanzaki, Yuki| Fujiwara, Atsushi| Morizane, Yuki|
Published Date 2020-12-09
Publication Title Scientific Reports
Volume volume10
Issue issue1
Publisher Nature Research
Start Page 21497
ISSN 2045-2322
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2020
File Version publisher
PubMed ID 33299123
DOI 10.1038/s41598-020-78693-5
Web of Science KeyUT 000608953600006
Related Url isVersionOf https://doi.org/10.1038/s41598-020-78693-5