FullText URL | fulltext.pdf |
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Author | Kondo, Yuki| Rikiishi, Kazuhide| Sugimoto, Manabu| |
Keywords | 8-oxo-dGTP nudix hydrolase Oryza sativa transcriptional error UV-C |
Published Date | 2022-09 |
Publication Title | Antioxidants |
Volume | volume11 |
Issue | issue9 |
Publisher | MDPI |
Start Page | 1805 |
ISSN | 2076-3921 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2022 by the authors. |
File Version | publisher |
PubMed ID | 36139879 |
DOI | 10.3390/antiox11091805 |
Web of Science KeyUT | 000858199700001 |
Related Url | isVersionOf https://doi.org/10.3390/antiox11091805 |
FullText URL | fulltext.pdf |
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Author | Sugimoto, Manabu| Watanabe, Toshiro| Takaoka, Motoko| Suzuki, Kyoko| Murakami, Tadatoshi| Murakami, Nobutada| Sumikawa, Shoichi| |
Keywords | fermented plant extract microbiota dextran sulfate sodium inflammatory Clostridiales |
Published Date | 2021-04-06 |
Publication Title | Fermentation-Basel |
Volume | volume7 |
Issue | issue2 |
Publisher | MDPI |
Start Page | 55 |
ISSN | 2311-5637 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2021 by the authors. |
File Version | publisher |
DOI | 10.3390/fermentation7020055 |
Web of Science KeyUT | 000665199500001 |
Related Url | isVersionOf https://doi.org/10.3390/fermentation7020055 |
FullText URL | fulltext20210616-1.pdf |
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Author | Rikiishi, Kazuhide| Sugimoto, Manabu| Maekawa, Masahiko| |
Keywords | mutant seed development seed dormancy transcriptome wheat |
Published Date | 2021-02-17 |
Publication Title | Breeding Science |
Volume | volume71 |
Issue | issue2 |
Publisher | Japanese Society of Breeding |
Start Page | 155 |
End Page | 166 |
ISSN | 1344-7610 |
NCID | AA11353132 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
NAID | 130008040484 |
DOI | 10.1270/jsbbs.20016 |
Web of Science KeyUT | 000654086400005 |
Related Url | isVersionOf https://doi.org/10.1270/jsbbs.20016 |
Author | Fujitani, Yoshiyuki| Horiuchi, Terumi| Ito, Kazutoshi| Sugimoto, Manabu| |
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Published Date | 2007-6 |
Publication Title | PHYTOCHEMISTRY |
Volume | volume68 |
Issue | issue11 |
Content Type | Journal Article |
Title Alternative | ホウレンソウ種子に存在するα-グルコシダーゼの分子多型変化 |
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FullText URL | 005_001_001_009.pdf |
Author | Furui, Satoshi| Sugimoto, Manabu| Suzuki, Yukio| |
Abstract | Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70 kDa by gel filtration, respectively. α-Glucosidase A had high activity not only toward maltooligosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 65℃ for 20 min. On the other hand, α-glucosidase B had high activity toward maltooligosaccharides but faint activity toward α-glucans. The optimum pH was 5.0 and no activity was found after incubation at 65℃ for 20 min. The enzymatic and immunological properties of α-glucosidase A and B were similar to those of α-glucosidase. Ⅰor Ⅱ, and α-glucosidase Ⅲ or Ⅳ, isolated from spinach seeds without 4℃ storage, respectively. These findings suggest that the α-glucosidase in spinach seeds is modified to be two molecular forms. |
Keywords | Spinach α-Glucosidase Multiple molecular forms |
Publication Title | 岡山大学資源生物科学研究所報告 |
Published Date | 1997 |
Volume | volume5 |
Issue | issue1 |
Start Page | 1 |
End Page | 9 |
ISSN | 0916-930X |
language | English |
File Version | publisher |
Title Alternative | シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 |
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FullText URL | 005_002_145_153.pdf |
Author | Sugimoto, Manabu| Kawai, Fusako| |
Abstract | A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. |
Keywords | Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression |
Publication Title | 岡山大学資源生物科学研究所報告 |
Published Date | 1998 |
Volume | volume5 |
Issue | issue2 |
Start Page | 145 |
End Page | 153 |
ISSN | 0916-930X |
language | English |
File Version | publisher |
Title Alternative | Purification and Characterization of α-Glucosidases from Spinach Seeds |
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FullText URL | 004_002_239_252.pdf |
Author | Sugimoto, Manabu| Furui, Satoshi| Suzuki, Yukio| |
Abstract | Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Ⅰand Ⅱ showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases Ⅲ and Ⅳ showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase Ⅲ serum precipitated specifically with α-glucosidase Ⅲ. |
Keywords | α-Glucosidase Spinach Seed Spinacia oleracea L. Molecular form |
Publication Title | 岡山大学資源生物科学研究所報告 |
Published Date | 1996 |
Volume | volume4 |
Issue | issue2 |
Start Page | 239 |
End Page | 252 |
ISSN | 0916-930X |
language | Japanese |
File Version | publisher |
Author | 杉本 学| |
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Published Date | 1994-03-25 |
Publication Title | |
Content Type | Thesis or Dissertation |