| Author | Kuroda, Masahiro| Asaumi, Junichi| Nishikawa, Koji| Tanaka, Seiryo| Gao, Xian Shu| Yamamoto, Michinori| Makihata, Eiichi| Hiraki, Yoshio| Kawasaki, Shoji| |
|---|---|
| Published Date | 1993-02 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume105 |
| Issue | issue1-2 |
| Content Type | Journal Article |
| Author | Gao, Xianshu| Nakagawa, Tomio| Yamamoto, Michinori| Kuroda, Masahiro| Sakae, Katsuyoshi| Shimizu, Mitsuharu| Niiya, Harutaka| Takeda, Yoshihiro| Joja, Ikuo| Hiraki, Yoshio| Kawasaki, Shoji| |
|---|---|
| Published Date | 1996-06-29 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume108 |
| Issue | issue3-6 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/11843 |
|---|---|
| Title Alternative | Relationship between intracellular uptake of adriamycin and membrane potential in ADR resistant Ehrlich ascites tumor cells |
| FullText URL | 003_081_085.pdf |
| Author | Asaumi, Jun-ichi| Kawasaki, Shoji| Nishikawa, Koji| Shu Gao, Xian| Kuroda, Masahiro| Hiraki, Yoshio| |
| Abstract | We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells. |
| Keywords | Adriamycin Cell Membrane Potential Flow Cytometry ADR-Resistant Cells DIDS |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1993-01-31 |
| Volume | volume3 |
| Start Page | 81 |
| End Page | 85 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313508 |
| JaLCDOI | 10.18926/11730 |
|---|---|
| Title Alternative | Measurement of intracellular pH by flow cytometry using pH sensitive fluorescence dye, and influence of hyperthermia and amiloride derivatives on the intracellular pH |
| FullText URL | 006_001_005.pdf |
| Author | Asaumi, Jun-ichi| Kawasaki, Shoji| Kuroda, Masahiro| Takeda, Yoshihiro| Hiraki, Yoshio| |
| Abstract | エールリッヒ腹水癌細胞とそのアドリアマイシン耐性細胞において蛍光pH指示薬2'、7'-bis-(2-carboxyethyl) carboxyfluorescein] (BCECF) の蛍光量をフローサイトメトリーで測定することによって細胞内pHの検量曲線を作成することができた。このことより、これらの細胞においてBCECFの蛍光量で細胞内pHの変化を簡易に比較できることを示唆した。さらに、温熱、Na(+)/H(+) exchanger の阻害例であるアミロライド[3,5-diamino-6-chloro-N-(diaminomethylene) pyrazinecarboxamide]、およびアミロライド誘導隊MH-12-43[N-amidino-3-amino-6-chloro-5-(N-ethyliso-propylamino) pyrazinecarboxyamide] の細胞内pHへの影響をエールリッヒ腹水癌細胞で観察した。37℃では、0.5mMアミロライド、0.05mM MH-12-43により細胞内pHは減少し、42℃処理によりさらに減少した。42℃において、0.05mM MH-12-43による細胞内pHの減少は、0.5mMアミロライドによる減少より大きかった。 |
| Keywords | BCECF 細胞内pH (Intracellular pH) フローサイトメトリー (Flow Cytometry) アミロライド (Amiloride) MH-12-43 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313855 |
| Author | 黒田 昌宏| |
|---|---|
| Published Date | 1993-12-31 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
