| FullText URL | fulltext.pdf |
|---|---|
| Author | Nakamitsu, Yuki| Kuroda, Masahiro| Shimizu, Yudai| Kuroda, Kazuhiro| Yoshimura, Yuuki| Yoshida, Suzuka| Nakamura, Yoshihide| Fukumura, Yuka| Kamizaki, Ryo| Al-Hammad, Wlla E.| Oita, Masataka| Tanabe, Yoshinori| Sugimoto, Kohei| Sugianto, Irfan| Barham, Majd| Tekiki, Nouha| Asaumi, Junichi| |
| Keywords | diffusion-weighted image Gaussian filter head and neck tumor magnetic resonance imaging mean kurtosis median filter nonlocal mean filter phantom simple diffusion kurtosis imaging restricted diffusion-weighted image |
| Published Date | 2024-03-20 |
| Publication Title | Journal of Clinical Medicine |
| Volume | volume13 |
| Issue | issue6 |
| Publisher | MDPI |
| Start Page | 1783 |
| ISSN | 2077-0383 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2024 by the authors. |
| File Version | publisher |
| PubMed ID | 38542005 |
| DOI | 10.3390/jcm13061783 |
| Web of Science KeyUT | 001192756600001 |
| Related Url | isVersionOf https://doi.org/10.3390/jcm13061783 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Imajo, Satoshi| Tanabe, Yoshinori| Nakamura, Nobue| Honda, Mitsugi| Kuroda, Masahiro| |
| Keywords | Quality improvement Signal-to-noise ratio Panoramic X-ray images Cluster analysis Occlusal plane |
| Note | The version of record of this article, first published in Egyptian Journal of Radiology and Nuclear Medicine, is available online at Publisher’s website: http://dx.doi.org/10.1186/s43055-023-01176-w| |
| Published Date | 2024-01-02 |
| Publication Title | Egyptian Journal of Radiology and Nuclear Medicine |
| Volume | volume55 |
| Issue | issue1 |
| Publisher | Springer |
| Start Page | 4 |
| ISSN | 2090-4762 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2023. |
| File Version | publisher |
| DOI | 10.1186/s43055-023-01176-w |
| Web of Science KeyUT | 001132999300005 |
| Related Url | isVersionOf https://doi.org/10.1186/s43055-023-01176-w |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Fukumura, Yuka| Kuroda, Masahiro| Yoshida, Suzuka| Nakamura, Yoshihide| Nakamitsu, Yuki| Al-Hammad, Wlla| Kuroda, Kazuhiro| Kamizaki, Ryo| Shimizu, Yudai| Tanabe, Yoshinori| Sugimoto, Kohei| Oita, Masataka| Sugianto, Irfan| Barham, Majd| Tekiki, Nouha| Kamaruddin, Nurul| Yanagi, Yoshinobu| Asaumi, Junichi| |
| Keywords | dentigerous cyst mean kurtosis simple diffusion kurtosis imaging head and neck magnetic resonance imaging apparent diffusion coefficient value diffusion kurtosis imaging |
| Published Date | 2023-12-07 |
| Publication Title | Diagnostics |
| Volume | volume13 |
| Issue | issue24 |
| Publisher | MDPI |
| Start Page | 3619 |
| ISSN | 2075-4418 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 by the authors. |
| File Version | publisher |
| PubMed ID | 38132203 |
| DOI | 10.3390/diagnostics13243619 |
| Web of Science KeyUT | 001131127700001 |
| Related Url | isVersionOf https://doi.org/10.3390/diagnostics13243619 |
| FullText URL | fulltext20231111-01.pdf |
|---|---|
| Author | Kamizaki, Ryo| Kuroda, Masahiro| Al‑Hammad, Wlla| Tekiki, Nouha| Ishizaka, Hinata| Kuroda, Kazuhiro| Sugimoto, Kohei| Oita, Masataka| Tanabe, Yoshinori| Barham, Majd| Sugianto, Irfan| Nakamitsu, Yuki| Hirano, Masaki| Muto, Yuki| Ihara, Hiroki| Sugiyama, Soichi| |
| Keywords | BC RT heart dose ML DNN DIBH |
| Note | This fulltext file will be available in Apr. 2024.| |
| Published Date | 2023-10-02 |
| Publication Title | Experimental and Therapeutic Medicine |
| Volume | volume26 |
| Issue | issue5 |
| Publisher | Spandidos Publications |
| Start Page | 536 |
| ISSN | 1792-0981 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © Spandidos Publications 2023. |
| File Version | publisher |
| PubMed ID | 37869640 |
| DOI | 10.3892/etm.2023.12235 |
| Web of Science KeyUT | 001086953300001 |
| Related Url | isVersionOf https://doi.org/10.3892/etm.2023.12235 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Sugimoto, Kohei| Oita, Masataka| Kuroda, Masahiro| |
| Keywords | MR image Image intensity standardization Windowing Prediction Bayesian statistical modeling |
| Published Date | 2023-08 |
| Publication Title | Heliyon |
| Volume | volume9 |
| Issue | issue8 |
| Publisher | Cell Press |
| Start Page | e19038 |
| ISSN | 2405-8440 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 The Authors. |
| File Version | publisher |
| PubMed ID | 37636402 |
| DOI | 10.1016/j.heliyon.2023.e19038 |
| Web of Science KeyUT | 001056714100001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.heliyon.2023.e19038 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Al-Hammad, Wlla E.| Kuroda, Masahiro| Kamizaki, Ryo| Tekiki, Nouha| Ishizaka, Hinata| Kuroda, Kazuhiro| Sugimoto, Kohei| Oita, Masataka| Tanabe, Yoshinori| Barham, Majd| Sugianto, Irfan| Shimizu, Yudai| Nakamitsu, Yuki| Asaumi, Junichi| |
| Keywords | breast cancer radiotherapy heart dose machine learning deep neural network deep inspiration breath-hold technique computed tomography |
| Published Date | 2023-08-04 |
| Publication Title | Current Oncology |
| Volume | volume30 |
| Issue | issue8 |
| Publisher | MDPI |
| Start Page | 7412 |
| End Page | 7424 |
| ISSN | 1198-0052 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 by the authors. |
| File Version | publisher |
| PubMed ID | 37623018 |
| DOI | 10.3390/curroncol30080537 |
| Web of Science KeyUT | 001054543000001 |
| Related Url | isVersionOf https://doi.org/10.3390/curroncol30080537 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Barham, Majd| Kuroda, Masahiro| Yoshimura, Yuuki| Hamada, Kentaro| Khasawneh, Abdullah| Sugimoto, Kohei| Konishi, Kohei| Tekiki, Nouha| Sugianto, Irfan| Bamgbose, Babatunde O.| Ishizaka, Hinata| Shimizu, Yudai| Nakamitsu, Yuki| Al-Hammad, Wlla E.| Kamizaki, Ryo| Kurozumi, Akira| Matsushita, Toshi| Ohno, Seiichiro| Asaumi, Junichi| |
| Published Date | 2023-02-27 |
| Publication Title | PLoS ONE |
| Volume | volume18 |
| Issue | issue2 |
| Publisher | Public Library of Science |
| ISSN | 1932-6203 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 Barham et al. |
| File Version | publisher |
| PubMed ID | 36848353 |
| DOI | 10.1371/journal.pone.0282462 |
| Web of Science KeyUT | 000996122900036 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0282462 |
| FullText URL | fulltext20230619-05.pdf |
|---|---|
| Author | Imajo, Satoshi| Tanabe, Yoshinori| Nakamura, Nobue| Honda, Mitsugi| Kuroda, Masahiro| |
| Keywords | panoramic radiography quantitative evaluation deformable image registration peak signal-to-noise ratio |
| Published Date | 2023-05-04 |
| Publication Title | Polish Journal of Medical Physics and Engineering |
| Volume | volume29 |
| Issue | issue2 |
| Publisher | Sciendo |
| Start Page | 85 |
| End Page | 91 |
| ISSN | 1898-0309 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 Satoshi Imajo et al. |
| File Version | publisher |
| DOI | 10.2478/pjmpe-2023-0010 |
| Web of Science KeyUT | 000981963000001 |
| Related Url | isVersionOf https://doi.org/10.2478/pjmpe-2023-0010 |
| JaLCDOI | 10.18926/AMO/65492 |
|---|---|
| FullText URL | 77_3_273.pdf |
| Author | Shimizu, Yudai| Kuroda, Masahiro| Nakamitsu, Yuki| Al-Hammad, Wlla E.| Yoshida, Suzuka| Fukumura, Yuka| Nakamura, Yoshihide| Kuroda, Kazuhiro| Kamizaki, Ryo| Imajoh, Satoshi| Tanabe, Yoshinori| Sugimoto, Kohei| Oita, Masataka| Sugianto, Irfan| Bamgbose, Babatunde O.| Yanagi, Yoshinobu| Asaumi, Junichi| |
| Abstract | Diffusion kurtosis (DK) imaging (DKI), a type of restricted diffusion-weighted imaging, has been reported to be useful for tumor diagnoses in clinical studies. We developed a software program to simultaneously create DK images with apparent diffusion coefficient (ADC) maps and conducted an initial clinical study. Multi-shot echo-planar diffusion-weighted images were obtained at b-values of 0, 400, and 800 sec/mm2 for simple DKI, and DK images were created simultaneously with the ADC map. The usefulness of the DK image and ADC map was evaluated using a pixel analysis of all pixels and a median analysis of the pixels of each case. Tumor and normal tissues differed significantly in both pixel and median analyses. In the pixel analysis, the area under the curve was 0.64 for the mean kurtosis (MK) value and 0.77 for the ADC value. In the median analysis, the MK value was 0.74, and the ADC value was 0.75. The MK and ADC values correlated moderately in the pixel analysis and strongly in the median analysis. Our simple DKI system created DK images simultaneously with ADC maps, and the obtained MK and ADC values were useful for differentiating head and neck tumors from normal tissue. |
| Keywords | simple diffusion kurtosis imaging mean kurtosis clinical trial head and neck tumor magnetic resonance imaging |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2023-06 |
| Volume | volume77 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 273 |
| End Page | 280 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2023 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 37357628 |
| Web of Science KeyUT | 001026279600005 |
| FullText URL | fulltext20230418-01.pdf |
|---|---|
| Author | Hirano, Masaki| Muto, Yuki| Kuroda, Masahiro| Fujiwara, Yuta| Sasaki, Tomoaki| Kuroda, Kazuhiro| Kamizaki, Ryo| Imajoh, Satoshi| Tanabe, Yoshinori| E. Al-Hammad, Wlla| Nakamitsu, Yuki| Shimizu, Yudai| Sugimoto, Kohei| Oita, Masataka| Sugianto, Irfan| O. Bamgbose, Babatunde| |
| Keywords | MAVRIC SL metal artifacts implant phantom MRI |
| Published Date | 2023-1-24 |
| Publication Title | Experimental and Therapeutic Medicine |
| Volume | volume25 |
| Issue | issue3 |
| Publisher | Spandidos Publications |
| Start Page | 109 |
| ISSN | 1792-0981 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © Hirano et al. |
| File Version | publisher |
| PubMed ID | 36793326 |
| DOI | 10.3892/etm.2023.11808 |
| Web of Science KeyUT | 000948969100001 |
| Related Url | isVersionOf https://doi.org/10.3892/etm.2023.11808 |
| JaLCDOI | 10.18926/AMO/63205 |
|---|---|
| FullText URL | 76_1_25.pdf |
| Author | Sugimoto, Kohei| Kuroda, Masahiro| Yoshimura, Yuuki| Hamada, Kentaro| Khasawneh, Abdullah| Barham, Majd| Tekiki, Nouha| Konishi, Kohei| Ishizaka, Hinata| Shimizu, Yudai| Nakamitsu, Yuki| Al-Hammad, Wlla E. | Kamizaki, Ryo| Kanazawa, Susumu| Asaumi, Junichi| |
| Abstract | The apparent diffusion coefficient subtraction method (ASM) was developed as a new restricted diffusionweighted imaging technique for magnetic resonance imaging (MRI). The usefulness of the ASM has been established by in vitro basic research using a bio-phantom, and clinical research on the application of the ASM for the human body is needed. Herein, we developed a short-time sequence for ASM imaging of the heads of healthy volunteers (n=2), and we investigated the similarity between the obtained ASM images and diffusion kurtosis (DK) images to determine the utility of the ASM for clinical uses. This study appears to be the first to report ASM images of the human head. We observed that the short-time sequence for the ASM imaging of the head can be scanned in approx. 3 min at 1.5T MRI. The noise reduction effect of median filter processing was confirmed on the ASM images scanned by this sequence. The obtained ASM images showed a weak correlation with the DK images, indicating that the ASM images are restricted diffusion-weighted images. The new shorttime imaging sequence could thus be used in clinical studies applying the ASM. |
| Keywords | apparent diffusion coefficient apparent diffusion coefficient subtraction method diffusion kurtosis imaging restricted diffusion short-time imaging |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-02 |
| Volume | volume76 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 25 |
| End Page | 32 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 35236995 |
| Web of Science KeyUT | 000762812700004 |
| FullText URL | fulltext20210824-1.pdf |
|---|---|
| Author | Tekiki, Nouha| Kuroda, Masahiro| Ishizaka, Hinata| Khasawneh, Abdullah| Barham, Majd| Hamada, Kentaro| Konishi, Kohei| Sugimoto, Kohei| Katsui, Kuniaki| Sugiyama, Soichi| Watanabe, Kenta| Yoshio, Kotaro| Katayama, Norihisa| Ogata, Takeshi| Ihara, Hiroki| Kanazawa, Susumu| Asaumi, Junichi| |
| Keywords | breast cancer field‑in‑field radiotherapy dose reference point acute skin toxicity dose distribution high‑dose area |
| Note | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.| |
| Published Date | 2021-7-21 |
| Publication Title | Molecular and Clinical Oncology |
| Volume | volume15 |
| Issue | issue3 |
| Publisher | Spandidos Publications |
| ISSN | 2049-9450 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © Tekiki et al. |
| File Version | publisher |
| PubMed ID | 34349992 |
| DOI | 10.3892/mco.2021.2355 |
| Web of Science KeyUT | 000680476900001 |
| Related Url | isVersionOf https://doi.org/10.3892/mco.2021.2355 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Katsui, Kuniaki| Ogata, Takeshi| Sugiyama, Soichi| Yoshio, Kotaro| Kuroda, Masahiro| Hiraki, Takao| Kiura, Katsuyuki| Maeda, Yoshinobu| Toyooka, Shinichi| Kanazawa, Susumu| |
| Published Date | 2021-06-04 |
| Publication Title | scientific reports |
| Volume | volume11 |
| Issue | issue1 |
| Publisher | Nature Research |
| Start Page | 11882 |
| ISSN | 2045-2322 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2021 |
| File Version | publisher |
| PubMed ID | 34088965 |
| DOI | 10.1038/s41598-021-91449-z |
| Web of Science KeyUT | 000678611200010 |
| Related Url | isVersionOf https://doi.org/10.1038/s41598-021-91449-z |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Watanabe, Kenta| Katsui, Kuniaki| Sugiyama, Soichiro| Yoshio, Kotaro| Kuroda, Masahiro| Hiraki, Takao| Kiura, Katsuyuki| Maeda, Yoshinobu| Toyooka, Shinichi| Kanazawa, Susumu| |
| Keywords | Clinical pathology Elderly Non-small cell lung carcinoma Radiosurgery Stereotactic body radiation therapy |
| Published Date | 2021-02-23 |
| Publication Title | Radiation Oncology |
| Volume | volume16 |
| Issue | issue1 |
| Publisher | BMC |
| Start Page | 39 |
| ISSN | 1748-717X |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2021. |
| File Version | publisher |
| PubMed ID | 33622369 |
| DOI | 10.1186/s13014-021-01769-7 |
| Web of Science KeyUT | 000623175000001 |
| Related Url | isVersionOf https://doi.org/10.1186/s13014-021-01769-7 |
| JaLCDOI | 10.18926/AMO/61429 |
|---|---|
| FullText URL | 75_1_15.pdf |
| Author | Katsui, Kuniaki| Ogata, Takeshi| Tada, Akihiro| Sugiyama, Soichi| Yoshio, Kotaro| Kuroda, Masahiro| Kiura, Katsuyuki| Maeda, Yoshinobu| Toyooka, Shinichi| Hiraki, Takao| Kanazawa, Susumu| |
| Abstract | The aim of this study was to investigate whether volumetric positron emission tomography (PET) parameters are prognostic predictors in stage III non-small cell lung cancer patients receiving definitive concurrent chemo-radiotherapy (CCRT) with cisplatin/docetaxel. Cases involving definitive CCRT were reviewed retrospectively, and the maximum standardized uptake value, metabolic tumor volume (MTV) and total lesion glycolysis (TLG) were calculated. The relationships between these PET parameters and prognosis were analyzed. MTV and TLG were significant predictors of distant metastasis-free survival (DMFS) (p = 0.0003 and 0.0005, respectively) and progression-free survival (PFS) (p = 0.001 and 0.0007, respectively). The three-year DMFS rates in patients with low and high MTV were 13.3% and 64.6%, respectively, and the corresponding values in those with low and high TLG were 13.3% and 65.2%, respectively. The three-year PFS rates in patients with low and high MTV were 13.3% and 57.8%, respectively, and the corresponding values in patients with low and high TLG were 13.3% and 57.8%, respectively. However, MTV and TLG were not predictors of local control or overall sur-vival. We demonstrated that volumetric PET parameters were predictors of patients receiving definitive CCRT. Our findings contradict the findings of previous reports and warrant further research to validate them. |
| Keywords | volumetric positron emission tomography parameters distant metastasis-free survival chemoradiotherapy cisplatin/docetaxel non-small cell lung cancer |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2021-02 |
| Volume | volume75 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 15 |
| End Page | 23 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2021 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 33649609 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Katsui, Kuniaki| Ogata, Takeshi| Watanabe, Kenta| Katayama, Norihisa| Kuroda, Masahiro| Kiura, Katsuyuki| Hiraki, Takao| Maeda, Yoshinobu| Toyooka, Shinichi| Kanazawa, Susumu| |
| Keywords | cisplatin docetaxel dose-volume histogram non-small cell lung cancer PACIFIC trial radiation pneumonitis |
| Published Date | 2020-05-04 |
| Publication Title | Cancer Medicine |
| Publisher | Wiley |
| ISSN | 2045-7634 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 The Authors. |
| File Version | publisher |
| PubMed ID | 32364685 |
| DOI | 10.1002/cam4.3093 |
| Web of Science KeyUT | 000530411600001 |
| Related Url | isVersionOf https://doi.org/10.1002/cam4.3093 |
| FullText URL | MMR20_3_2963.pdf |
|---|---|
| Author | Yoshimura, Yuuki| Kuroda, Masahiro| Sugianto, Irfan| Khasawneh, Abdullah| Bamgbose, Babatunde O.| Hamada, Kentaro| Barham, Majd| Tekiki, Nouha| Kurozumi, Akira| Matsushita, Toshi| Ohno, Seiichiro| Kanazawa, Susumu| Asaumi, Junichi| |
| Keywords | magnetic resonance imaging apparent diffusion coefficient diffusion kurtosis imaging subtraction restricted diffusion bio-phantom cell |
| Published Date | 2019-07-25 |
| Publication Title | Molecular Medicine Reports |
| Volume | volume20 |
| Issue | issue3 |
| Publisher | Spandidos Publications |
| Start Page | 2963 |
| End Page | 2969 |
| ISSN | 1791-2997 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| PubMed ID | 31524240 |
| DOI | 10.3892/mmr.2019.10523 |
| Web of Science KeyUT | 000482435500103 |
| Related Url | isVersionOf https://doi.org/10.3892/mmr.2019.10523 |
| JaLCDOI | 10.18926/AMO/52009 |
|---|---|
| FullText URL | 67_6_359.pdf |
| Author | Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi| |
| Abstract | It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis. |
| Keywords | ADC apoptosis necrosis hyperthermia cell density |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-12 |
| Volume | volume67 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 359 |
| End Page | 367 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 24356720 |
| Web of Science KeyUT | 000328915700004 |
| JaLCDOI | 10.18926/AMO/48964 |
|---|---|
| FullText URL | 66_5_399.pdf |
| Author | Jing, Shao-Wu| Wang, Ya-Di| Kuroda, Masahiro| Su, Jing-Wei| Sun, Guo-Gui| Liu, Qing| Cheng, Yun-Jie| Yang, Cong-Rong| |
| Abstract | Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma. The goal of this study was to explore the probable mechanism of HIF-1α in the invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo. mRNA and protein expression of HIF-1α, E-cadherin and matrix metalloproteinase-2 (MMP-2) under hypoxia were detected by RT-PCR and Western blotting. The effects of silencing HIF-1α on E-cadherin, MMP-2 mRNA and protein expression under hypoxia or normoxia were detected by RT-PCR and Western blotting, respectively. The invasive ability of Eca109 cells was tested using a transwell chambers. We established an Eca109-implanted tumor model and observed tumor growth and lymph node metastasis. The expression of HIF-1α, E-cadherin and MMP-2 in xenograft tumors was detected by Western blotting. After exposure to hypoxia, HIF-1α protein was up-regulated, both mRNA and protein levels of E-cadherin were down-regulated and MMP-2 was up-regulated, while HIF-1α mRNA showed no significant change. SiRNA could block HIF-1α effectively, increase E-cadherin expression and inhibit MMP-2 expression. The number of invading cells decreased after HIF-1α was silenced. Meanwhile, the tumor volume was much smaller, and the metastatic rate of lymph nodes and the positive rate were lower in vivo. Our observations suggest that HIF-1α inhibition might be an effective strategy to weaken invasion and metastasis in the esophageal carcinoma Eca109 cell line. |
| Keywords | hypoxia-inducible factor-1α esophageal carcinoma invasion and metastasis |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-10 |
| Volume | volume66 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 399 |
| End Page | 407 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23093058 |
| Web of Science KeyUT | 000310253900004 |
| JaLCDOI | 10.18926/AMO/48566 |
|---|---|
| FullText URL | 66_3_263.pdf |
| Author | Sasaki, Takanori| Kuroda, Masahiro| Katashima, Kazunori| Ashida, Masakazu| Matsuzaki, Hidenobu| Asaumi, Junichi| Murakami, Jun| Ohno, Seiichiro| Kato, Hirokazu| Kanazawa, Susumu| |
| Abstract | The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittｾs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values. |
| Keywords | apparent diffusion coefficient value cell density extracellular space bio-phantom |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-06 |
| Volume | volume66 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 263 |
| End Page | 270 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22729107 |
| Web of Science KeyUT | 000305669700010 |
