黒田昌宏

Enhancing Diagnostic Precision: Evaluation of Preprocessing Filters in Simple Diffusion Kurtosis Imaging for Head and Neck Tumors

フルテキストURL	fulltext.pdf
著者	Nakamitsu, Yuki\| Kuroda, Masahiro\| Shimizu, Yudai\| Kuroda, Kazuhiro\| Yoshimura, Yuuki\| Yoshida, Suzuka\| Nakamura, Yoshihide\| Fukumura, Yuka\| Kamizaki, Ryo\| Al-Hammad, Wlla E.\| Oita, Masataka\| Tanabe, Yoshinori\| Sugimoto, Kohei\| Sugianto, Irfan\| Barham, Majd\| Tekiki, Nouha\| Asaumi, Junichi\|
キーワード	diffusion-weighted image Gaussian filter head and neck tumor magnetic resonance imaging mean kurtosis median filter nonlocal mean filter phantom simple diffusion kurtosis imaging restricted diffusion-weighted image
発行日	2024-03-20
出版物タイトル	Journal of Clinical Medicine
巻	13巻
号	6号
出版者	MDPI
開始ページ	1783
ISSN	2077-0383
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2024 by the authors.
論文のバージョン	publisher
PubMed ID	38542005
DOI	10.3390/jcm13061783
Web of Science KeyUT	001192756600001
関連URL	isVersionOf https://doi.org/10.3390/jcm13061783

Evaluating the index of panoramic X-ray image quality using K-means clustering method

フルテキストURL	fulltext.pdf
著者	Imajo, Satoshi\| Tanabe, Yoshinori\| Nakamura, Nobue\| Honda, Mitsugi\| Kuroda, Masahiro\|
キーワード	Quality improvement Signal-to-noise ratio Panoramic X-ray images Cluster analysis Occlusal plane
備考	The version of record of this article, first published in Egyptian Journal of Radiology and Nuclear Medicine, is available online at Publisher’s website: http://dx.doi.org/10.1186/s43055-023-01176-w\|
発行日	2024-01-02
出版物タイトル	Egyptian Journal of Radiology and Nuclear Medicine
巻	55巻
号	1号
出版者	Springer
開始ページ	4
ISSN	2090-4762
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© The Author(s) 2023.
論文のバージョン	publisher
DOI	10.1186/s43055-023-01176-w
Web of Science KeyUT	001132999300005
関連URL	isVersionOf https://doi.org/10.1186/s43055-023-01176-w

Characteristic Mean Kurtosis Values in Simple Diffusion Kurtosis Imaging of Dentigerous Cysts

フルテキストURL	fulltext.pdf
著者	Fukumura, Yuka\| Kuroda, Masahiro\| Yoshida, Suzuka\| Nakamura, Yoshihide\| Nakamitsu, Yuki\| Al-Hammad, Wlla\| Kuroda, Kazuhiro\| Kamizaki, Ryo\| Shimizu, Yudai\| Tanabe, Yoshinori\| Sugimoto, Kohei\| Oita, Masataka\| Sugianto, Irfan\| Barham, Majd\| Tekiki, Nouha\| Kamaruddin, Nurul\| Yanagi, Yoshinobu\| Asaumi, Junichi\|
キーワード	dentigerous cyst mean kurtosis simple diffusion kurtosis imaging head and neck magnetic resonance imaging apparent diffusion coefficient value diffusion kurtosis imaging
発行日	2023-12-07
出版物タイトル	Diagnostics
巻	13巻
号	24号
出版者	MDPI
開始ページ	3619
ISSN	2075-4418
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 by the authors.
論文のバージョン	publisher
PubMed ID	38132203
DOI	10.3390/diagnostics13243619
Web of Science KeyUT	001131127700001
関連URL	isVersionOf https://doi.org/10.3390/diagnostics13243619

Evaluation of the accuracy of heart dose prediction by machine learning for selecting patients not requiring deep inspiration breath‑hold radiotherapy after breast cancer surgery

フルテキストURL	fulltext20231111-01.pdf
著者	Kamizaki, Ryo\| Kuroda, Masahiro\| Al‑Hammad, Wlla\| Tekiki, Nouha\| Ishizaka, Hinata\| Kuroda, Kazuhiro\| Sugimoto, Kohei\| Oita, Masataka\| Tanabe, Yoshinori\| Barham, Majd\| Sugianto, Irfan\| Nakamitsu, Yuki\| Hirano, Masaki\| Muto, Yuki\| Ihara, Hiroki\| Sugiyama, Soichi\|
キーワード	BC RT heart dose ML DNN DIBH
備考	This fulltext file will be available in Apr. 2024.\|
発行日	2023-10-02
出版物タイトル	Experimental and Therapeutic Medicine
巻	26巻
号	5号
出版者	Spandidos Publications
開始ページ	536
ISSN	1792-0981
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© Spandidos Publications 2023.
論文のバージョン	publisher
PubMed ID	37869640
DOI	10.3892/etm.2023.12235
Web of Science KeyUT	001086953300001
関連URL	isVersionOf https://doi.org/10.3892/etm.2023.12235

Bayesian statistical modeling to predict observer-specific optimal windowing parameters in magnetic resonance imaging

フルテキストURL	fulltext.pdf
著者	Sugimoto, Kohei\| Oita, Masataka\| Kuroda, Masahiro\|
キーワード	MR image Image intensity standardization Windowing Prediction Bayesian statistical modeling
発行日	2023-08
出版物タイトル	Heliyon
巻	9巻
号	8号
出版者	Cell Press
開始ページ	e19038
ISSN	2405-8440
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 The Authors.
論文のバージョン	publisher
PubMed ID	37636402
DOI	10.1016/j.heliyon.2023.e19038
Web of Science KeyUT	001056714100001
関連URL	isVersionOf https://doi.org/10.1016/j.heliyon.2023.e19038

Mean Heart Dose Prediction Using Parameters of Single-Slice Computed Tomography and Body Mass Index: Machine Learning Approach for Radiotherapy of Left-Sided Breast Cancer of Asian Patients

フルテキストURL	fulltext.pdf
著者	Al-Hammad, Wlla E.\| Kuroda, Masahiro\| Kamizaki, Ryo\| Tekiki, Nouha\| Ishizaka, Hinata\| Kuroda, Kazuhiro\| Sugimoto, Kohei\| Oita, Masataka\| Tanabe, Yoshinori\| Barham, Majd\| Sugianto, Irfan\| Shimizu, Yudai\| Nakamitsu, Yuki\| Asaumi, Junichi\|
キーワード	breast cancer radiotherapy heart dose machine learning deep neural network deep inspiration breath-hold technique computed tomography
発行日	2023-08-04
出版物タイトル	Current Oncology
巻	30巻
号	8号
出版者	MDPI
開始ページ	7412
終了ページ	7424
ISSN	1198-0052
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 by the authors.
論文のバージョン	publisher
PubMed ID	37623018
DOI	10.3390/curroncol30080537
Web of Science KeyUT	001054543000001
関連URL	isVersionOf https://doi.org/10.3390/curroncol30080537

Evaluation of calculation processes of apparent diffusion coefficient subtraction method (ASM) imaging

フルテキストURL	fulltext.pdf
著者	Barham, Majd\| Kuroda, Masahiro\| Yoshimura, Yuuki\| Hamada, Kentaro\| Khasawneh, Abdullah\| Sugimoto, Kohei\| Konishi, Kohei\| Tekiki, Nouha\| Sugianto, Irfan\| Bamgbose, Babatunde O.\| Ishizaka, Hinata\| Shimizu, Yudai\| Nakamitsu, Yuki\| Al-Hammad, Wlla E.\| Kamizaki, Ryo\| Kurozumi, Akira\| Matsushita, Toshi\| Ohno, Seiichiro\| Asaumi, Junichi\|
発行日	2023-02-27
出版物タイトル	PLoS ONE
巻	18巻
号	2号
出版者	Public Library of Science
ISSN	1932-6203
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 Barham et al.
論文のバージョン	publisher
PubMed ID	36848353
DOI	10.1371/journal.pone.0282462
Web of Science KeyUT	000996122900036
関連URL	isVersionOf https://doi.org/10.1371/journal.pone.0282462

Objective evaluation method using multiple image analyses for panoramic radiography improvement

フルテキストURL	fulltext20230619-05.pdf
著者	Imajo, Satoshi\| Tanabe, Yoshinori\| Nakamura, Nobue\| Honda, Mitsugi\| Kuroda, Masahiro\|
キーワード	panoramic radiography quantitative evaluation deformable image registration peak signal-to-noise ratio
発行日	2023-05-04
出版物タイトル	Polish Journal of Medical Physics and Engineering
巻	29巻
号	2号
出版者	Sciendo
開始ページ	85
終了ページ	91
ISSN	1898-0309
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 Satoshi Imajo et al.
論文のバージョン	publisher
DOI	10.2478/pjmpe-2023-0010
Web of Science KeyUT	000981963000001
関連URL	isVersionOf https://doi.org/10.2478/pjmpe-2023-0010

Usefulness of Simple Diffusion Kurtosis Imaging for Head and Neck Tumors: An Early Clinical Study

JaLCDOI	10.18926/AMO/65492
フルテキストURL	77_3_273.pdf
著者	Shimizu, Yudai\| Kuroda, Masahiro\| Nakamitsu, Yuki\| Al-Hammad, Wlla E.\| Yoshida, Suzuka\| Fukumura, Yuka\| Nakamura, Yoshihide\| Kuroda, Kazuhiro\| Kamizaki, Ryo\| Imajoh, Satoshi\| Tanabe, Yoshinori\| Sugimoto, Kohei\| Oita, Masataka\| Sugianto, Irfan\| Bamgbose, Babatunde O.\| Yanagi, Yoshinobu\| Asaumi, Junichi\|
抄録	Diffusion kurtosis (DK) imaging (DKI), a type of restricted diffusion-weighted imaging, has been reported to be useful for tumor diagnoses in clinical studies. We developed a software program to simultaneously create DK images with apparent diffusion coefficient (ADC) maps and conducted an initial clinical study. Multi-shot echo-planar diffusion-weighted images were obtained at b-values of 0, 400, and 800 sec/mm2 for simple DKI, and DK images were created simultaneously with the ADC map. The usefulness of the DK image and ADC map was evaluated using a pixel analysis of all pixels and a median analysis of the pixels of each case. Tumor and normal tissues differed significantly in both pixel and median analyses. In the pixel analysis, the area under the curve was 0.64 for the mean kurtosis (MK) value and 0.77 for the ADC value. In the median analysis, the MK value was 0.74, and the ADC value was 0.75. The MK and ADC values correlated moderately in the pixel analysis and strongly in the median analysis. Our simple DKI system created DK images simultaneously with ADC maps, and the obtained MK and ADC values were useful for differentiating head and neck tumors from normal tissue.
キーワード	simple diffusion kurtosis imaging mean kurtosis clinical trial head and neck tumor magnetic resonance imaging
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2023-06
巻	77巻
号	3号
出版者	Okayama University Medical School
開始ページ	273
終了ページ	280
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	Copyright Ⓒ 2023 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	37357628
Web of Science KeyUT	001026279600005

Quantitative evaluation of the reduction of distortion and metallic artifacts in magnetic resonance images using the multiacquisition variable‑resonance image combination selective sequence

フルテキストURL	fulltext20230418-01.pdf
著者	Hirano, Masaki\| Muto, Yuki\| Kuroda, Masahiro\| Fujiwara, Yuta\| Sasaki, Tomoaki\| Kuroda, Kazuhiro\| Kamizaki, Ryo\| Imajoh, Satoshi\| Tanabe, Yoshinori\| E. Al-Hammad, Wlla\| Nakamitsu, Yuki\| Shimizu, Yudai\| Sugimoto, Kohei\| Oita, Masataka\| Sugianto, Irfan\| O. Bamgbose, Babatunde\|
キーワード	MAVRIC SL metal artifacts implant phantom MRI
発行日	2023-1-24
出版物タイトル	Experimental and Therapeutic Medicine
巻	25巻
号	3号
出版者	Spandidos Publications
開始ページ	109
ISSN	1792-0981
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© Hirano et al.
論文のバージョン	publisher
PubMed ID	36793326
DOI	10.3892/etm.2023.11808
Web of Science KeyUT	000948969100001
関連URL	isVersionOf https://doi.org/10.3892/etm.2023.11808

Development and Evaluation of a Short-time Imaging Method for the Clinical Study of the Apparent Diffusion Coefficient Subtraction Method

JaLCDOI	10.18926/AMO/63205
フルテキストURL	76_1_25.pdf
著者	Sugimoto, Kohei\| Kuroda, Masahiro\| Yoshimura, Yuuki\| Hamada, Kentaro\| Khasawneh, Abdullah\| Barham, Majd\| Tekiki, Nouha\| Konishi, Kohei\| Ishizaka, Hinata\| Shimizu, Yudai\| Nakamitsu, Yuki\| Al-Hammad, Wlla E. \| Kamizaki, Ryo\| Kanazawa, Susumu\| Asaumi, Junichi\|
抄録	The apparent diffusion coefficient subtraction method (ASM) was developed as a new restricted diffusionweighted imaging technique for magnetic resonance imaging (MRI). The usefulness of the ASM has been established by in vitro basic research using a bio-phantom, and clinical research on the application of the ASM for the human body is needed. Herein, we developed a short-time sequence for ASM imaging of the heads of healthy volunteers (n=2), and we investigated the similarity between the obtained ASM images and diffusion kurtosis (DK) images to determine the utility of the ASM for clinical uses. This study appears to be the first to report ASM images of the human head. We observed that the short-time sequence for the ASM imaging of the head can be scanned in approx. 3 min at 1.5T MRI. The noise reduction effect of median filter processing was confirmed on the ASM images scanned by this sequence. The obtained ASM images showed a weak correlation with the DK images, indicating that the ASM images are restricted diffusion-weighted images. The new shorttime imaging sequence could thus be used in clinical studies applying the ASM.
キーワード	apparent diffusion coefficient apparent diffusion coefficient subtraction method diffusion kurtosis imaging restricted diffusion short-time imaging
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2022-02
巻	76巻
号	1号
出版者	Okayama University Medical School
開始ページ	25
終了ページ	32
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	Copyright Ⓒ 2022 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	35236995
Web of Science KeyUT	000762812700004

New field‑in‑field with two reference points method for whole breast radiotherapy: Dosimetric analysis and radiation‑induced skin toxicities assessment

フルテキストURL	fulltext20210824-1.pdf
著者	Tekiki, Nouha\| Kuroda, Masahiro\| Ishizaka, Hinata\| Khasawneh, Abdullah\| Barham, Majd\| Hamada, Kentaro\| Konishi, Kohei\| Sugimoto, Kohei\| Katsui, Kuniaki\| Sugiyama, Soichi\| Watanabe, Kenta\| Yoshio, Kotaro\| Katayama, Norihisa\| Ogata, Takeshi\| Ihara, Hiroki\| Kanazawa, Susumu\| Asaumi, Junichi\|
キーワード	breast cancer field‑in‑field radiotherapy dose reference point acute skin toxicity dose distribution high‑dose area
備考	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.\|
発行日	2021-7-21
出版物タイトル	Molecular and Clinical Oncology
巻	15巻
号	3号
出版者	Spandidos Publications
ISSN	2049-9450
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© Tekiki et al.
論文のバージョン	publisher
PubMed ID	34349992
DOI	10.3892/mco.2021.2355
Web of Science KeyUT	000680476900001
関連URL	isVersionOf https://doi.org/10.3892/mco.2021.2355

Sarcopenia is associated with poor prognosis after chemoradiotherapy in patients with stage III non-small-cell lung cancer: a retrospective analysis

フルテキストURL	fulltext.pdf
著者	Katsui, Kuniaki\| Ogata, Takeshi\| Sugiyama, Soichi\| Yoshio, Kotaro\| Kuroda, Masahiro\| Hiraki, Takao\| Kiura, Katsuyuki\| Maeda, Yoshinobu\| Toyooka, Shinichi\| Kanazawa, Susumu\|
発行日	2021-06-04
出版物タイトル	scientific reports
巻	11巻
号	1号
出版者	Nature Research
開始ページ	11882
ISSN	2045-2322
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© The Author(s) 2021
論文のバージョン	publisher
PubMed ID	34088965
DOI	10.1038/s41598-021-91449-z
Web of Science KeyUT	000678611200010
関連URL	isVersionOf https://doi.org/10.1038/s41598-021-91449-z

Lung stereotactic body radiation therapy for elderly patients aged >= 80 years with pathologically proven early-stage non-small cell lung cancer: a retrospective cohort study

フルテキストURL	fulltext.pdf
著者	Watanabe, Kenta\| Katsui, Kuniaki\| Sugiyama, Soichiro\| Yoshio, Kotaro\| Kuroda, Masahiro\| Hiraki, Takao\| Kiura, Katsuyuki\| Maeda, Yoshinobu\| Toyooka, Shinichi\| Kanazawa, Susumu\|
キーワード	Clinical pathology Elderly Non-small cell lung carcinoma Radiosurgery Stereotactic body radiation therapy
発行日	2021-02-23
出版物タイトル	Radiation Oncology
巻	16巻
号	1号
出版者	BMC
開始ページ	39
ISSN	1748-717X
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© The Author(s) 2021.
論文のバージョン	publisher
PubMed ID	33622369
DOI	10.1186/s13014-021-01769-7
Web of Science KeyUT	000623175000001
関連URL	isVersionOf https://doi.org/10.1186/s13014-021-01769-7

Volumetric PET Parameters Predict Prognosis after Definitive Chemoradiotherapy with Cisplatin/Docetaxel for Stage III Non-Small Cell Lung Cancer

JaLCDOI	10.18926/AMO/61429
フルテキストURL	75_1_15.pdf
著者	Katsui, Kuniaki\| Ogata, Takeshi\| Tada, Akihiro\| Sugiyama, Soichi\| Yoshio, Kotaro\| Kuroda, Masahiro\| Kiura, Katsuyuki\| Maeda, Yoshinobu\| Toyooka, Shinichi\| Hiraki, Takao\| Kanazawa, Susumu\|
抄録	The aim of this study was to investigate whether volumetric positron emission tomography (PET) parameters are prognostic predictors in stage III non-small cell lung cancer patients receiving definitive concurrent chemo-radiotherapy (CCRT) with cisplatin/docetaxel. Cases involving definitive CCRT were reviewed retrospectively, and the maximum standardized uptake value, metabolic tumor volume (MTV) and total lesion glycolysis (TLG) were calculated. The relationships between these PET parameters and prognosis were analyzed. MTV and TLG were significant predictors of distant metastasis-free survival (DMFS) (p = 0.0003 and 0.0005, respectively) and progression-free survival (PFS) (p = 0.001 and 0.0007, respectively). The three-year DMFS rates in patients with low and high MTV were 13.3% and 64.6%, respectively, and the corresponding values in those with low and high TLG were 13.3% and 65.2%, respectively. The three-year PFS rates in patients with low and high MTV were 13.3% and 57.8%, respectively, and the corresponding values in patients with low and high TLG were 13.3% and 57.8%, respectively. However, MTV and TLG were not predictors of local control or overall sur-vival. We demonstrated that volumetric PET parameters were predictors of patients receiving definitive CCRT. Our findings contradict the findings of previous reports and warrant further research to validate them.
キーワード	volumetric positron emission tomography parameters distant metastasis-free survival chemoradiotherapy cisplatin/docetaxel non-small cell lung cancer
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2021-02
巻	75巻
号	1号
出版者	Okayama University Medical School
開始ページ	15
終了ページ	23
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2021 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	33649609

Radiation pneumonitis after definitive concurrent chemoradiotherapy with cisplatin/docetaxel for non-small cell lung cancer: Analysis of dose-volume parameters

フルテキストURL	fulltext.pdf
著者	Katsui, Kuniaki\| Ogata, Takeshi\| Watanabe, Kenta\| Katayama, Norihisa\| Kuroda, Masahiro\| Kiura, Katsuyuki\| Hiraki, Takao\| Maeda, Yoshinobu\| Toyooka, Shinichi\| Kanazawa, Susumu\|
キーワード	cisplatin docetaxel dose-volume histogram non-small cell lung cancer PACIFIC trial radiation pneumonitis
発行日	2020-05-04
出版物タイトル	Cancer Medicine
出版者	Wiley
ISSN	2045-7634
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2020 The Authors.
論文のバージョン	publisher
PubMed ID	32364685
DOI	10.1002/cam4.3093
Web of Science KeyUT	000530411600001
関連URL	isVersionOf https://doi.org/10.1002/cam4.3093

Development of a novel method for visualizing restricted diffusion using subtraction of apparent diffusion coefficient values

フルテキストURL	MMR20_3_2963.pdf
著者	Yoshimura, Yuuki\| Kuroda, Masahiro\| Sugianto, Irfan\| Khasawneh, Abdullah\| Bamgbose, Babatunde O.\| Hamada, Kentaro\| Barham, Majd\| Tekiki, Nouha\| Kurozumi, Akira\| Matsushita, Toshi\| Ohno, Seiichiro\| Kanazawa, Susumu\| Asaumi, Junichi\|
キーワード	magnetic resonance imaging apparent diffusion coefficient diffusion kurtosis imaging subtraction restricted diffusion bio-phantom cell
発行日	2019-07-25
出版物タイトル	Molecular Medicine Reports
巻	20巻
号	3号
出版者	Spandidos Publications
開始ページ	2963
終了ページ	2969
ISSN	1791-2997
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
論文のバージョン	publisher
PubMed ID	31524240
DOI	10.3892/mmr.2019.10523
Web of Science KeyUT	000482435500103
関連URL	isVersionOf https://doi.org/10.3892/mmr.2019.10523

In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Jurkat Cells Using Bio-phantoms

JaLCDOI	10.18926/AMO/52009
フルテキストURL	67_6_359.pdf
著者	Katashima, Kazunori\| Kuroda, Masahiro\| Ashida, Masakazu\| Sasaki, Takanori\| Taguchi, Takehito\| Matsuzaki, Hidenobu\| Murakami, Jun\| Yanagi, Yoshinobu\| Hisatomi, Miki\| Hara, Marina\| Kato, Hirokazu\| Ohmura, Yuichi\| Kobayashi, Tomoki\| Kanazawa, Susumu\| Harada, Sosuke\| Takemoto, Mitsuhiro\| Ohno, Seiichiro\| Mimura, Seiichi\| Asaumi, Junichi\|
抄録	It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.
キーワード	ADC apoptosis necrosis hyperthermia cell density
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2013-12
巻	67巻
号	6号
出版者	Okayama University Medical School
開始ページ	359
終了ページ	367
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	24356720
Web of Science KeyUT	000328915700004

HIF-1α Contributes to Hypoxia-induced Invasion and Metastasis of Esophageal Carcinoma via Inhibiting E-cadherin and Promoting MMP-2 Expression

JaLCDOI	10.18926/AMO/48964
フルテキストURL	66_5_399.pdf
著者	Jing, Shao-Wu\| Wang, Ya-Di\| Kuroda, Masahiro\| Su, Jing-Wei\| Sun, Guo-Gui\| Liu, Qing\| Cheng, Yun-Jie\| Yang, Cong-Rong\|
抄録	Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma. The goal of this study was to explore the probable mechanism of HIF-1α in the invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo. mRNA and protein expression of HIF-1α, E-cadherin and matrix metalloproteinase-2 (MMP-2) under hypoxia were detected by RT-PCR and Western blotting. The effects of silencing HIF-1α on E-cadherin, MMP-2 mRNA and protein expression under hypoxia or normoxia were detected by RT-PCR and Western blotting, respectively. The invasive ability of Eca109 cells was tested using a transwell chambers. We established an Eca109-implanted tumor model and observed tumor growth and lymph node metastasis. The expression of HIF-1α, E-cadherin and MMP-2 in xenograft tumors was detected by Western blotting. After exposure to hypoxia, HIF-1α protein was up-regulated, both mRNA and protein levels of E-cadherin were down-regulated and MMP-2 was up-regulated, while HIF-1α mRNA showed no significant change. SiRNA could block HIF-1α effectively, increase E-cadherin expression and inhibit MMP-2 expression. The number of invading cells decreased after HIF-1α was silenced. Meanwhile, the tumor volume was much smaller, and the metastatic rate of lymph nodes and the positive rate were lower in vivo. Our observations suggest that HIF-1α inhibition might be an effective strategy to weaken invasion and metastasis in the esophageal carcinoma Eca109 cell line.
キーワード	hypoxia-inducible factor-1α esophageal carcinoma invasion and metastasis
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2012-10
巻	66巻
号	5号
出版者	Okayama University Medical School
開始ページ	399
終了ページ	407
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	23093058
Web of Science KeyUT	000310253900004

In Vitro Assessment of Factors Affecting the Apparent Diffusion Coefficient of Ramos Cells Using Bio-phantoms

JaLCDOI	10.18926/AMO/48566
フルテキストURL	66_3_263.pdf
著者	Sasaki, Takanori\| Kuroda, Masahiro\| Katashima, Kazunori\| Ashida, Masakazu\| Matsuzaki, Hidenobu\| Asaumi, Junichi\| Murakami, Jun\| Ohno, Seiichiro\| Kato, Hirokazu\| Kanazawa, Susumu\|
抄録	The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittｾs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.
キーワード	apparent diffusion coefficient value cell density extracellular space bio-phantom
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2012-06
巻	66巻
号	3号
出版者	Okayama University Medical School
開始ページ	263
終了ページ	270
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	22729107
Web of Science KeyUT	000305669700010

黒田 昌宏