JaLCDOI 10.18926/AMO/30405
フルテキストURL fulltext.pdf
著者 Tamura, Tomoyuki| Koide, Norio| Hada, Hajime| Shiraha, Hidenori| Tsuji, Takao|
抄録 Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other.
キーワード hepatocyte spheroid primary culture gene expression IL-6
Amo Type Article
発行日 1995-06
出版物タイトル Acta Medica Okayama
49巻
3号
出版者 Okayama University Medical School
開始ページ 161
終了ページ 167
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7676847
Web of Sience KeyUT A1995RH05400007
著者 Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio|
発行日 2005-01-20
出版物タイトル BMC Cancer
5巻
資料タイプ 学術雑誌論文
著者 小林 功幸| 中村 進一郎| 大西 秀樹| 歳森 淳一| 桑木 健志| 萩原 宏明| 三宅 康広| 白羽 英則| 能祖 一裕| 八木 孝仁| 田中 紀章| 山本 和秀|
発行日 2009-04-01
出版物タイトル 岡山医学会雑誌
121巻
1号
資料タイプ 学術雑誌論文
著者 藤川 達也| 白羽 英則| 山本 和秀|
発行日 2009-04-01
出版物タイトル 岡山医学会雑誌
121巻
1号
資料タイプ 学術雑誌論文
著者 白羽 英則|
発行日 1996-03-31
出版物タイトル
資料タイプ 学位論文