著者 Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio|
発行日 2005-01-20
出版物タイトル BMC Cancer
5巻
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/49042
フルテキストURL 66_6_461.pdf
著者 Koike, Kazuko| Takaki, Akinobu| Kato, Nobuyuki| Ouchida, Mamoru| Kanzaki, Hirotaka| Yasunaka, Tetsuya| Shiraha, Hidenori| Miyake, Yasuhiro| Yamamoto, Kazuhide|
抄録 Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression.
キーワード hepatitis C virus retinol-binding protein
Amo Type Original Article
発行日 2012-12
出版物タイトル Acta Medica Okayama
66巻
6号
出版者 Okayama University Medical School
開始ページ 461
終了ページ 468
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 23254580
Web of Sience KeyUT 000312966100005
著者 藤川 達也| 白羽 英則| 山本 和秀|
発行日 2009-04-01
出版物タイトル 岡山医学会雑誌
121巻
1号
資料タイプ 学術雑誌論文
著者 Matsubara, Minoru| Shiraha, Hidenori| Kataoka, Jyunro| Iwamuro, Masaya| Horiguchi, Shigeru| Nishina, Shin-ichi| Takaoka, Nobuyuki| Uemura, Masayuki| Takaki, Akinobu| Nakamura, Shinichiro| Kobayashi, Yoshiyuki| Nouso, Kazuhiro| Yamamoto, Kazuhide|
発行日 2012-10
出版物タイトル Journal of Gastroenterology and Hepatology
27巻
10号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32110
フルテキストURL fulltext.pdf
著者 Shinji, Toshiyuki| Kyaw, Yi Yi| Gokan, Katsunori| Tanaka, Yasuhito| Ochi, Koji| Kusano, Nobuchika| Mizushima, Takaaki| Fujioka, Shin-ichi| Shiraha, Hidenori| Lwin, Aye Aye| Shiratori, Yasushi| Mizokami, Masashi| Khin, Myo| Miyahara, Masayuki| Okada, Shigeru| Koide, Norio|
抄録 The prevalence of hepatitis C virus (HCV) genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR) amplified. Among them, 35 (31.8%) were of genotype 1, 52 (47.3%) were of genotype 3, and 23 (20.9%) were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.
キーワード hepatitis C virus(HCV)genotype type 6 variant Myanmar Southeast Asia phylogenetic analysis
Amo Type Article
発行日 2004-06
出版物タイトル Acta Medica Okayama
58巻
3号
出版者 Okayama University Medical School
開始ページ 135
終了ページ 142
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 15471435
Web of Sience KeyUT 000222273300004