| JaLCDOI | 10.18926/AMO/30405 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Tamura, Tomoyuki| Koide, Norio| Hada, Hajime| Shiraha, Hidenori| Tsuji, Takao| |
| 抄録 | Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other. |
| キーワード | hepatocyte spheroid primary culture gene expression IL-6 |
| Amo Type | Article |
| 発行日 | 1995-06 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 49巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 161 |
| 終了ページ | 167 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 7676847 |
| Web of Sience KeyUT | A1995RH05400007 |
| JaLCDOI | 10.18926/AMO/49042 |
|---|---|
| フルテキストURL | 66_6_461.pdf |
| 著者 | Koike, Kazuko| Takaki, Akinobu| Kato, Nobuyuki| Ouchida, Mamoru| Kanzaki, Hirotaka| Yasunaka, Tetsuya| Shiraha, Hidenori| Miyake, Yasuhiro| Yamamoto, Kazuhide| |
| 抄録 | Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression. |
| キーワード | hepatitis C virus retinol-binding protein |
| Amo Type | Original Article |
| 発行日 | 2012-12 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 66巻 |
| 号 | 6号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 461 |
| 終了ページ | 468 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 著作権者 | CopyrightⒸ 2012 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 23254580 |
| Web of Sience KeyUT | 000312966100005 |
| JaLCDOI | 10.18926/AMO/52898 |
|---|---|
| フルテキストURL | 68_5_291.pdf |
| 著者 | Tsuzaki, Ryuichiro| Takaki, Akinobu| Yagi, Takahito| Ikeda, Fusao| Koike, Kazuko| Iwasaki, Yoshiaki| Shiraha, Hidenori| Miyake, Yasuhiro| Sadamori, Hiroshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Nobuoka, Daisuke| Utsumi, Masashi| Nakayama, Eiichi| Fujiwara, Toshiyoshi| Yamamoto, Kazuhide| |
| 抄録 | It is not known how the immune system targets hepatitis C virus (HCV)-infected HLA-mismatched hepatocytes under immune-suppressed conditions after orthotopic liver transplantation (OLT). In addition, the relationship between the HCV-specific immune response and IL28B variants as predictors of HCV clearance has not been well-characterized. We determined the IL28B polymorphisms for 57 post-OLT HCV carriers, and we assessed the HCV-specific immune responses by measuring the peripheral blood mononuclear cell-derived HCV-specific interferon-gamma (IFN-γ) response using an enzyme-linked immunospot assay. At 1-3 years after OLT, patients with no active hepatitis showed higher total spots on the immunospot assay. At＞3 years after OLT, patients with resolved HCV showed higher levels of core, NS3, NS5A, and total spots compared to the chronic hepatitis patients. The IL28B major genotype in the donors correlated with higher spot counts for NS5A and NS5B proteins at 1-3 years after OLT. In the post-OLT setting, the HCV-specific immune response could be strongly induced in patients with no active hepatitis with an IL28B major donor or sustained virological response. Strong immune responses in the patients with no active hepatitis could only be maintained for 3 years and diminished later. It may be beneficial to administer IFN treatment starting 3 years after OLT, to induce the maximum immunological effect. |
| キーワード | interferon gamma ELISPOT assay single nucleotide polymorphisms dendritic cell CD4 T cell |
| Amo Type | Original Article |
| 発行日 | 2014-10 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 68巻 |
| 号 | 5号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 291 |
| 終了ページ | 302 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 著作権者 | CopyrightⒸ 2014 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 25338486 |
| Web of Sience KeyUT | 000343269300006 |
| 関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/53129 |
| JaLCDOI | 10.18926/AMO/53026 |
|---|---|
| フルテキストURL | 68_6_369.pdf |
| 著者 | Iwamuro, Masaya| Miyashima, Yuichi| Yoshioka, Takahiro| Murata, Toshihiro| Miyabe, Yoshio| Kawai, Yoshinari| Urata, Haruo| Shiraha, Hidenori| Okada, Hiroyuki| Yamamoto, Kazuhide| |
| 抄録 | A 67-year-old Japanese man underwent enterotomy because of enterolith ileus. Component analysis by infrared spectroscopy revealed that the enterolith was composed of a high concentration of deoxycholic acid. We further analyzed and compared the ultrastructure of the enterolith and a commercially available powdered form of deoxycholic acid by means of scanning electron microscopy and energy dispersive X-ray spectroscopy. Energy dispersive X-ray spectroscopy analysis revealed that the ratios of carbon and oxygen in the enterolith were equal to those in the deoxycholic acid powder. Scanning electron microscopy analysis showed rectangular prism-shaped particles on the surface of the enterolith. This structure was similar to that of the deoxycholic acid powder. The surgically removed enterolith had a twisted and coiled appearance. Possible mechanisms underlying the formation of this unique form are discussed. |
| キーワード | enterolith deoxycholic acid scanning electron microscopy infrared spectroscopy energy dispersive X-ray spectroscopy |
| Amo Type | Case Report |
| 発行日 | 2014-12 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 68巻 |
| 号 | 6号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 369 |
| 終了ページ | 374 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 著作権者 | CopyrightⒸ 2014 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 25519031 |
| Web of Sience KeyUT | 000346882200007 |
| タイトル（別表記） | Fermented persimmon extract (kaki-shibu) is useful as a standard for component analyses of persimmon phytobezoars |
|---|---|
| フルテキストURL | 126_127.pdf |
| 著者 | 岩室 雅也| 岡本 裕子| 村田 年弘| 河合 良成| 白羽 英則| 岡田 裕之| 山本 和秀| |
| 抄録 | The definite diagnosis of persimmon phytobezoar (i.e., diospyrobezoar) is often accomplished by a component analysis using infrared spectroscopy. However, no studies have been conducted to investigate which substance is the best as a standard for the component analysis. Here we analyzed tannic acid, Japanese persimmon (kaki), fermented persimmon extract (kaki-shibu), conventional dried persimmon, and dried persimmon smoked in sulfur (ampo-kaki) by infrared spectroscopy to determine which would be optimal as a component analysis standard. The spectrum between 1,600 to 600cm－1 of a persimmon phytobezoar was quite similar to the spectrum of kaki-shibu rather than that of tannic acid. Consequently, we conclude that kaki-shibu should be used as a standard for infrared spectroscopy analyses of persimmon phytobezoars. |
| キーワード | 柿胃石（gastric phytobezoar） タンニン酸（tannic acid） 消化管異物（gastrointestinal foreign body） 成分分析（component analysis） |
| 出版物タイトル | 岡山医学会雑誌 |
| 発行日 | 2014-08-01 |
| 巻 | 126巻 |
| 号 | 2号 |
| 開始ページ | 127 |
| 終了ページ | 131 |
| ISSN | 0030-1558 |
| 言語 | Japanese |
| 著作権者 | Copyright (c) 2014 岡山医学会 |
| 論文のバージョン | publisher |
| DOI | 10.4044/joma.126.127 |
| NAID | 130004685263 |