JaLCDOI 10.18926/AMO/30405
FullText URL fulltext.pdf
Author Tamura, Tomoyuki| Koide, Norio| Hada, Hajime| Shiraha, Hidenori| Tsuji, Takao|
Abstract Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other.
Keywords hepatocyte spheroid primary culture gene expression IL-6
Amo Type Article
Published Date 1995-06
Publication Title Acta Medica Okayama
Volume volume49
Issue issue3
Publisher Okayama University Medical School
Start Page 161
End Page 167
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7676847
Web of Sience KeyUT A1995RH05400007
Author Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio|
Published Date 2005-01-20
Publication Title BMC Cancer
Volume volume5
Content Type Journal Article
Author Kobayashi, Yoshiyuki| Nakamura, Shinichiro| Ohnishi, Hideki| Toshimori, Junichi| Kuwaki, Kenji| Hagihara, Hiroaki| Miyake, Yasuhiro| Shiraha, Hidenori| Nouso, Kazuhiro| Yagi, Takahito| Tanaka, Noriaki| Yamamoto, Kazuhide|
Published Date 2009-04-01
Publication Title 岡山医学会雑誌
Volume volume121
Issue issue1
Content Type Journal Article
Author Fujikawa, Tatsuya| Shiraha, Hidenori| Yamamoto, Kazuhide|
Published Date 2009-04-01
Publication Title 岡山医学会雑誌
Volume volume121
Issue issue1
Content Type Journal Article
Author 白羽 英則|
Published Date 1996-03-31
Publication Title
Content Type Thesis or Dissertation