result 18185 件
| Title Alternative | Detection of the Viruses Occurring in Oriental Cymbidium in Japan |
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| FullText URL | 004_002_149_162.pdf |
| Author | Kondo, Hideki| Maeda, Takanori| Mitsuhata, Koji| Inouye, Narinobu| |
| Abstract | A survey of virus diseases occurring in Oriental Cymbidium collected from a commerical nursery and home garden in Japan was conducted in 1991-1994. Identification of the vurus was based on partcle morphology, symptomatology in indicator plants, ultrastructure of infected cells and serology. Four viruses, odontoglossum ringspot tabamovirus(ORSV), cymbidium mosaic potexvirus(CyMV), orchid fleck virus (ORV) and a previously underscribed spherical virus, were found in 27 out of 37 Cymbidium plants tested. ORSV was detected from 11 plants belinging to Cym. ensifolium, Cym. forrestii, Cym. goeringii, Cym. kanran, Cym. sinense and Cymbidium spp. showing chlorotic streaks and/or mild mosaic. CyMV was isolated from only one plant of Cymbidium sp. showing mosaic and necrotic spots on leaves. In negatibvely stained dip preparations from plants infected with ORSV and CyMV, rod shaped particles of ca. 310 nm and flexuous rod-shaped ca. 475 nm in length were observed, respectively. The viruses were reacted strongly with respective antiserum to each virus in immunosorbent electron microcopy and inderect ELISA. OFV was isolated from four plants of Cym. formosanum, Cym. kanran, Cym. sinense and Cymbidium sp. showing mosaic and necrotic flecks. The virus had non-enveloped, bullet-shaped particles about 40×120～150 nm in dip preparation. The undescribed spherical virus, ca. 28 nm diameter, was isolated from 11 plants of Cym. forrestii, Cym. goeringii and Cymbidium spp. showing stunting and chlorotic streaks on newly developed leaves. The virus was mechanically transmitted only to Cymbidium orchids. Previously, we designated it as cymbidium chlorotic mosaic sobemovirus(CyCMV)(Kondo et al,1994),as the virus was considered to be a new member of the genus Sobemovirsu. |
| Keywords | Oriental Cymbidium Odontoglossum ringspot tobamovirus Cymbidium mosaic potexvirus Orchid fleck virus Cymbidium chlorotic mosaic sobemovirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 149 |
| End Page | 162 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313470 |
| Title Alternative | カクヤリグサ科一年生雑草における数量分類学的研究 |
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| FullText URL | 002_001_123_134.pdf |
| Author | Muhamad Ahmad, Chozin| Satou, Kazuhiro| Yasuda, Shozo| |
| Abstract | Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. |
| Keywords | Cyperus iria Cyperus microiria Cyperus amuricus Numerical taxonomy Speciation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 123 |
| End Page | 134 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313639 |
| Title Alternative | オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較 |
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| FullText URL | 004_002_137_147.pdf |
| Author | Ikegami, Masato| Inouye, Narinobu| |
| Abstract | The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%. |
| Keywords | Tobamovirus Odontoglossum ringspot virus Nucleotide sequence Genome organization |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 137 |
| End Page | 147 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Comparison of Resistance to Powdery Mildew between Wild Barley (Hordeum spontaneum C. Koch) |
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| FullText URL | 002_001_111_122.pdf |
| Author | Fukuyama, Toshinori| Heta, Hideo| Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | A total of 162 strains of wild barley, Hordeum spontaneum C. Koch originating from Iran, Iraq, Turkey and Central Asia, were tested for resistance to powdery mildew. Then, the variation of resistance was compared with that of 145 local varieties of cultivated barley (Hordeum vulgare L.) originating from the same region of the wild barley collection. Ten different isolates of the parasite with Japanese origin were separately inoculated onto the first leaves of the host plants. The infection types were classified into the following: i, immunelike; R, highly resistant; M, moderately resistant; and S, highly susceptible. Resistant strains with i, R or M infection type were more frequent among wild barleys as compared with the cultivated forms. It is noteworthy that among these three resistant reactions,the M type was most frequent in the wild barley. To compare the degree of resistance to a total of 10 isolates, the resistance score was calculated in each of the wild and cultivated strains as the following: Scores 1,2,3 and 4 were given to the infection types of i, R, M and S, respectively, and the mean score for 10 isolates was calculated. Wild barley showed significantly low resistance scores as compared with those of cultivated barley. This was also confirmed by the cluster analysis; the cluster with more resistance to 10 isolates consisted of many strains of wild barley. Next, the resistance of wild barley was characterized by their broader effective ranges to different isolates. According to the x2 test for independence of reactions to two different isolates, the resistant factor(s) involved in wild barley was confirmed to be rather non-specific to the parasite. It was concluded that H. spontaneum may be useful genetic resources for the breeding of resistance to powdery mildew as well as local varieties. |
| Keywords | Hordeum spontaneum Barley Powdery mildew Resistance |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 111 |
| End Page | 122 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313607 |
| Title Alternative | Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe |
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| FullText URL | 004_002_119_135.pdf |
| Author | Inouye, Narinobu| Matsumoto, Jun-ichi| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| |
| Abstract | Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV･So from Cymbidium. |
| Keywords | Calanthe Orchid fleck virus Calanthe yellowish fleck disease |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 119 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314037 |
| Title Alternative | 大麦うどんこ病菌(Erysiphe graminis DC. f. sp. hordei)の地理的分化 |
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| FullText URL | 002_001_103_110.pdf |
| Author | Konishi, Takeo| Heta, Hideo| |
| Abstract | Fifteen cultures of barley powdery mildew fungus (Erysiphe graminis DC. f. sp. hordei) isolated in different regions of the world were inoculated to 120 barley varieties. Infection scores varied among the cultures and barley varieties, and their interactions were observed. Principal component analysis of the infection scores revealed that the cultures could be classified into three groups by first and second components (contributing 40.2% and 15.6% of the total variance, respectively), and that two Japanese cultures could be distinguished from the others isolated in Europe and North America. This indicates that the fungus is geographically differentiated in the reaction of the barley varieties to the cultures. Furthermore, East Asian barley varieties differed from European ones in their reactions to the culltures, while barley varieties from regions between East Asia and Europe showed a large genetic diversity in their reactions. |
| Keywords | Barley Powdery mildew Geographic distribution Differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 103 |
| End Page | 110 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
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| FullText URL | 003_002_163_174.pdf |
| Author | Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| Keywords | Odontoglossum ringspot virus Calanthe sp. Identification |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 163 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Sources of Resistance to Net Blotch in Barley Germplasm |
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| FullText URL | 002_001_091_102.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
| Keywords | Barley Net blotch Disease resistance Genetic resources Race differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 91 |
| End Page | 102 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313965 |
| Title Alternative | Diallel Analysis for the Percentage of Grains with Hull Rupture in F2 Populations of Two-rowed Barley |
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| FullText URL | 002_001_079_089.pdf |
| Author | Wu, Jiri| Takeda, Kazuyoshi| Kanatani, Ryoichi| |
| Abstract | Half diallel F2 populations crossed among eight two-rowed barley varieties with warious percentages of hull ruptured grains were raised to analyze the inheritance of the trait. The percentage of grains with hull rupture varied from 0 to 87% among the parents. The diallel analysis revealed the following: the trait was predominantly controlled by the additive genes, and the dominance effect of some parents was also significant. However, the epistatic effect of the genes was not significant. The average dominance was 0.97. The heritability value was estimated as 0.57 and 0.91 in a narrow and a broad sense, respectivelly. The percentage of grains with hull rupture showed continuous and transgressive segregations in 28 F2 populations derived from half diallel crosses among eight parents. Heritability of the trait in a broad sense was 0.43~0.80(0.65 on average) in 28 F2 populations. |
| Keywords | Barley Grain quality Hull rupture Diallel analysis Heritability |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 79 |
| End Page | 89 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Host Range and Some Properties of Orchid Fleck Virus Isolated form Oriental Cymbidium in Japan |
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| FullText URL | 003_002_151_161.pdf |
| Author | Kondo, Hideki| Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Orchid flck virus (OFV) was isolated from Oriental Cymbidium (Cymbidium sp.),showing chlorotic flecks on leaves. The virus was transmitted mechanically to Chenopodium quinoa,C.murale and Beta vulgaris by sap-inoculation and caused systemic infection. Local lesions were produced on C.amaranticolar, Petunia hybrida, Tetragonia expansa and Vigna sinensis. Sap from infected T.expansa was still infective after 10 mim at 40℃ but not after 10 min at 45℃, at a dilution of 10-3 but not 10-4, and after 30 min at room temperature but not after 60 min. The isolate of OFV had non-enveloped, bullet-shaped patricles measuring about 40×120－150 nm in dip preparations. However, bacilliform particles about 40×120-140 nm were observed in ultrathin sections. In ultrahtin sections of virus-infected tissues, virus patricles were detected both in the nuclei and in the cytoplasm. Moreover, the inclusions of low electron density(viroplasm) were also observed in the nuclei.Virus particles were found to attach at one end to the inner nuclear membrane. A number of particles surrounded by the inner membrane often showed an appearance like a spoked wheel. |
| Keywords | Orchid fleck virus Oriental Cymbidium Non-enveloped rhabdovirus like particles |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 151 |
| End Page | 161 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313940 |
| Title Alternative | Character Expression and Inheritance of a "Short Upper Leaves" Mutant in Barley |
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| FullText URL | 002_001_069_078.pdf |
| Author | Kanatani, Ryoichi| Wu, Jiri| Takeda, Kazuyoshi| |
| Abstract | A mutant which devolops an extremely shortened flag leaf and shorter upper leaves was obtained from the progeny of 90 KR of gamma-ray irradiated Fuji Nijo, a malting barley variety. This mutant was controlled by a single partial dominant gene named Sul (short upper leaves). The character expression and the inheritance of the gene were investigated in this study. Sul shortened the flag leaf and upper several leaves and uppermost internode length, while it did not affect the length of spike, lower leaves and the second or lower internodes. A reciprocal translocation of the chromosomes was observed in this mutant line, and the Sull gene was linked with the V (two-rowed) gene on chromosome 2, and also linked with the breaking point of the reciprocal translocation. Small leaf area caused by the Sull gene might reduce the mutual shading and the transpiration of the leaf canopy at the later growth stages. |
| Keywords | Barley Plant type Linkage analysis Developmental pattern |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 69 |
| End Page | 78 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum |
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| FullText URL | 003_002_145_149.pdf |
| Author | Katsuhara, Maki| Bohnert, Hans J.| |
| Abstract | Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists. |
| Keywords | Mesembryanthemum crystallinum Potassium channel RT-PCR |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 145 |
| End Page | 149 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification |
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| FullText URL | 003_002_137_143.pdf |
| Author | Akiyama, Yoshiko| Shibasaka, Mineo| Kawasaki, Toshio| |
| Abstract | Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged. |
| Keywords | Adenylate extraction ADP AMP ATP Barley roots |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 137 |
| End Page | 143 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Effects of Mannosen on Hydroponically Grown Barley |
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| FullText URL | 003_002_129_135.pdf |
| Author | Shibasaka, Mineo| Miyata, Masahiko| Akiyama, Yoshiko| Kawasaki, Toshio| |
| Abstract | To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. |
| Keywords | Barley Hydroponics Mannose Potassium Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 129 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Genetic Analysis of Large Trichome in Barley Leaf Blade |
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| FullText URL | 002_001_063_068.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | The inheritance and linkage relationship of a new hairiness trait "large trichome" was investigated in barley. Although the size of large trichome is about four times that of normal one, the character can not be recognized with the naked eye. However, it is easily identified by the roughness of leaf touch. The large trichomes develop on both sides of the leaf blades. The direction of trichome is both acropetal and basipetal. It is clearly distinguished from the extremely long trichome controlled by Pub gene. About 2,300 varieties of our Barley Germplasm Center were screened by the leaf touch to find nine varieties with large trichome. Two of them were six-rowed local variety from Pakistan, and other seven were two-rowed varieties from Europe and Japan. All of them were hulled type. Crosses of six large trichome varieties with a normal Japanese variety resulted in the large trichome type F1s, suggesting the dominant nature of the trait. The large trichome line Hokuiku 17 was crossed with various linkage testres to study the mode of inheritance and the linkage relationship of the gene. In the F2 populations, the large trichome was controlled by a single dominant gene named Ltr (large trichome), which was independentiy inherited from the following marker genes; br and gl-5 on chromosome 1; li and ν on chromosome 2; uz on chromosome 3; K and gl-3 on chromosome 4; trd on chromosome 5; ο on chromosome 6. On the other hand, from the cross between Hokuiku 17 and OUL166, Ltr was found to be linked with s and fs on chromosome 7. Although the allelism test has not been completed, the very low frequency of the large trichome type (9/2,300) indicates that the variant resulted from a recent mutation event, or the fitness of the variant is low in the natural and/or artificial selection. |
| Keywords | Barley Trichome Linkage analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 63 |
| End Page | 68 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313663 |
| Title Alternative | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
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| FullText URL | 003_002_117_127.pdf |
| Author | Yonetani, Tsutomu| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
| Keywords | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 117 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Inheritance of Phenol Reaction in the Awn of Barley |
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| FullText URL | 002_001_055_062.pdf |
| Author | Takeda, Kazuyoshi| Zhang, Chan Lin| Kanatani, Ryoichi| |
| Abstract | The inheritance and geographical distribution of the phenol reaction in rice has been investigated intensively. On the other hand, in the case of barley, almost all of the varieties show positive reaction to phenol, and inheritance study of the trait has not yet been reported. We investigated the phenol reaction of ca. 5,000 barley varieties preserved at the Barley Germplasm Center of Okayama University to find about 30 varieties which show a negative reaction to the phenol. These varieties are mainly from Southwest Asia. The reaction of awn was sharpest at all parts of the plant including seeds. The positive reaction must be the prototype of barley, because Hordeum spontaneum, a possible ancestor of the cultivated barley, shows positive reaction. Inheritance and linkage studies were conducted using varieties with negative reaction and linkage testers with positive reaction. Phenol reaction was dominant and showed a clear 3:1 segregation ratio in the F2 populations, indicating the trait was controlled by a single dominant gene Ph(phenol reaction). Linkage study revealed that Ph was linked with four marker genes(three loci) on the chromosome 2 and independent of 13 other marker genes located on the chromosomes except chromosome 2. Ph may be a useful marker gene and an interesting material for molecular-biological studies. |
| Keywords | Barley Phenol reaction Linkage analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 55 |
| End Page | 62 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Effect of Light Irradiation on the Absorption of Rubidium in Chlorella |
|---|---|
| FullText URL | 003_002_105_116.pdf |
| Author | Hachiya, Kinji| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The effect of light irradiation on the absorption of rubidium was examined in Chlorella. Rubidium absorption in Chlorella was clearly stimulated by the irradiation of light. To clarify the mechanism of light-stimulation on rubidium absorption, experiments were carried out using several metabolic inhibitors; DCMU, NaCN,DNP,CCCP and ouabain. Among the metabolic inhibitors used, DCMU had the most similar effects on the rubidium absorption and oxygen evolution under light condition. These findings suggest a close correlation between the light-stimulation on rubidium absorption and the photosynthetic process in Chlorella. |
| Keywords | Chlorella Light-stimulation Metabolic inhibitors Oxygen evolution Rubidium absorption |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 116 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley |
|---|---|
| FullText URL | 002_001_043_053.pdf |
| Author | Mano, Yoshiro| Rikiishi, Kazuhide| Yasuda, Shozo| |
| Abstract | Immature embryos of 99 varieties of barley were cultured to investigate the ability of callus growth and plant regeneration. These two in vitro traits showed wide and continuous variations among the barley varieties tested. Ability of callus growth, which were evaluated by callus diameter ranged from 3.9mm to 11.2mm, and ability of plant regeneration from the calli ranged from 0% to 100%. A set of complete diallel crosses was made using six cultivars as the parents which differed in ability of callus growth and plant regeneration. The Vr/Wr graphical analysis showed that there were epistasis, or interaction among nonallelic genes for callus growth. As to ability of plant regeneration, no epistasis existed in the subdiallel without P1 (J232) which showed high specific combining ability, and it was controlled by a simple additive dominance genetic system. The mean degree of dominance(0.42) was relatively low and the broad(0.86) and narrow(0.78) sense heritabilities were high. |
| Keywords | Barley Tissue culture Plant regeneration Diallel analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 53 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
|---|---|
| FullText URL | 003_002_091_103.pdf |
| Author | Moritsugu, Masumi| Kawasaki, Toshio| Suzuki, Takao| |
| Abstract | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
| Keywords | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 091 |
| End Page | 103 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
