ID | 58552 |
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Author |
Kanazawa, Mai
Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University
Ikeda, Yoko
Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University
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Nishihama, Ryuichi
Graduate School of Biostudies, Kyoto University
Yamaoka, Shohei
Graduate School of Biostudies, Kyoto University
Lee, Nam-Hee
Department of Life Sciences, Faculty of Science and Engineering, Sorbonne University
Yamato, Katsuyuki T
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kindai University
Kohchi, Takayuk
Graduate School of Biostudies, Kyoto University
Hirayama, Takashi
Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University
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Abstract | Regulation of the stability and the quality of mitochondrial RNA is essential for the maintenance of mitochondrial and cellular functions in eukaryotes. We have previously reported that the eukaryotic poly(A)-specific ribonuclease (PARN) and the prokaryotic poly(A) polymerase encoded by AHG2 and AGS1, respectively, coordinately regulate the poly(A) status and the stability of mitochondrial mRNA in Arabidopsis. Mitochondrial function of PARN has not been reported in any other eukaryotes. To know how much this PARN-based mitochondrial mRNA regulation is conserved among plants, we studied the AHG2 and AGS1 counterparts of the liverwort, Marchantia polymorpha, a member of basal land plant lineage. We found that M. polymorpha has one ortholog each for AHG2 and AGS1, named MpAHG2 and MpAGS1, respectively. Their Citrine-fused proteins were detected in mitochondria of the liverwort. Molecular genetic analysis showed that MpAHG2 is essential and functionally interacts with MpAGS1 as observed in Arabidopsis. A recombinant MpAHG2 protein had a deadenylase activity in vitro. Overexpression of MpAGS1 and the reduced expression of MpAHG2 caused an accumulation of polyadenylated Mpcox1 mRNA. Furthermore, MpAHG2 suppressed Arabidopsis ahg2-1 mutant phenotype. These results suggest that the PARN-based mitochondrial mRNA regulatory system is conserved in land plants.
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Keywords | Arabidopsis
Marchantia polymorpha
Mitochondria
Poly(A) polymerase
Poly(A) regulation
Poly(A)-specific ribonuclease
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Note | This is a pre-copyedited, author-produced version of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record Mai Kanazawa et. al. Regulation of the Poly(A) Status of Mitochondrial mRNA by Poly(A)-Specific Ribonuclease Is Conserved among Land Plants. Plant and Cell Physiology 61(3), 470–480 (2020) is available online at: https://doi.org/10.1093/pcp/pcz212.
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Published Date | 2019-11-13
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Publication Title |
Plant and Cell Physiology
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Volume | volume61
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Issue | issue3
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Publisher | Oxford University Press
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Start Page | 470
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End Page | 480
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ISSN | 00320781
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NCID | AA0077511X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1093/pcp/pcz212
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | 5H04383
19K22434
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