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ID 58552
著者
Kanazawa, Mai Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University
Ikeda, Yoko Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University Kaken ID researchmap
Nishihama, Ryuichi Graduate School of Biostudies, Kyoto University
Yamaoka, Shohei Graduate School of Biostudies, Kyoto University
Lee, Nam-Hee Department of Life Sciences, Faculty of Science and Engineering, Sorbonne University
Yamato, Katsuyuki T Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kindai University
Kohchi, Takayuk Graduate School of Biostudies, Kyoto University
Hirayama, Takashi Division of Science for Bioresources, Graduate School of Environment and Life Science, Okayama University ORCID Kaken ID publons researchmap
抄録
Regulation of the stability and the quality of mitochondrial RNA is essential for the maintenance of mitochondrial and cellular functions in eukaryotes. We have previously reported that the eukaryotic poly(A)-specific ribonuclease (PARN) and the prokaryotic poly(A) polymerase encoded by AHG2 and AGS1, respectively, coordinately regulate the poly(A) status and the stability of mitochondrial mRNA in Arabidopsis. Mitochondrial function of PARN has not been reported in any other eukaryotes. To know how much this PARN-based mitochondrial mRNA regulation is conserved among plants, we studied the AHG2 and AGS1 counterparts of the liverwort, Marchantia polymorpha, a member of basal land plant lineage. We found that M. polymorpha has one ortholog each for AHG2 and AGS1, named MpAHG2 and MpAGS1, respectively. Their Citrine-fused proteins were detected in mitochondria of the liverwort. Molecular genetic analysis showed that MpAHG2 is essential and functionally interacts with MpAGS1 as observed in Arabidopsis. A recombinant MpAHG2 protein had a deadenylase activity in vitro. Overexpression of MpAGS1 and the reduced expression of MpAHG2 caused an accumulation of polyadenylated Mpcox1 mRNA. Furthermore, MpAHG2 suppressed Arabidopsis ahg2-1 mutant phenotype. These results suggest that the PARN-based mitochondrial mRNA regulatory system is conserved in land plants.
キーワード
Arabidopsis
Marchantia polymorpha
Mitochondria
Poly(A) polymerase
Poly(A) regulation
Poly(A)-specific ribonuclease
備考
This fulltext is available in Nov. 2020.
発行日
2019-11-13
出版物タイトル
Plant and Cell Physiology
61巻
3号
出版者
Oxford University Press
開始ページ
470
終了ページ
480
ISSN
00320781
NCID
AA0077511X
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1093/pcp/pcz212
助成機関名
文部科学省
助成番号
5H04383
19K22434