| ID | 30527 |
| JaLCDOI | |
| FullText URL | |
| Author |
Oda, Takuzo
Watanabe, Sekiko
Hanakawa, Shiro
Nakamura, Takashi
|
| Abstract | A permeable cell system has been developed by treatment with digitonin for studying in vitro DNA replication of chromatin. DNA replication of simian virus 40 nucleoprotein complexes (SV40 chromatin) in digitonin-treated permeable cells was analyzed by electrophoresis in agarose-gel. Autoradiography of the agarose-gel revealed that [32P]dCTP was incorporated in SV40 DNA I, II and replicating intermediates. The time course of the incorporation indicated the complete replication of SV40 DNA and chromatin with a full number of nucleosomes. The digitonin-treated permeable cell system will serve as a useful system for studying in vitro DNA replication of chromatin. |
| Keywords | digitonin
permeable cells
DNA replication in vitro
SV40 chromatin replication
gel -electrophoresis
autoradiography
|
| Amo Type | Brief Note
|
| Publication Title |
Acta Medica Okayama
|
| Published Date | 1980-12
|
| Volume | volume34
|
| Issue | issue6
|
| Publisher | Okayama University Medical School
|
| Start Page | 409
|
| End Page | 413
|
| ISSN | 0386-300X
|
| NCID | AA00508441
|
| Content Type |
Journal Article
|
| language |
English
|
| File Version | publisher
|
| Refereed |
True
|
| PubMed ID | |
| Web of Science KeyUT |
