| ID | 30527 |
| JaLCDOI | |
| フルテキストURL | |
| 著者 |
Oda, Takuzo
Okayama University
Watanabe, Sekiko
Okayama University
Hanakawa, Shiro
Okayama University
Nakamura, Takashi
Okayama University
|
| 抄録 | A permeable cell system has been developed by treatment with digitonin for studying in vitro DNA replication of chromatin. DNA replication of simian virus 40 nucleoprotein complexes (SV40 chromatin) in digitonin-treated permeable cells was analyzed by electrophoresis in agarose-gel. Autoradiography of the agarose-gel revealed that [32P]dCTP was incorporated in SV40 DNA I, II and replicating intermediates. The time course of the incorporation indicated the complete replication of SV40 DNA and chromatin with a full number of nucleosomes. The digitonin-treated permeable cell system will serve as a useful system for studying in vitro DNA replication of chromatin. |
| キーワード | digitonin
permeable cells
DNA replication in vitro
SV40 chromatin replication
gel -electrophoresis
autoradiography
|
| Amo Type | Brief Note
|
| 出版物タイトル |
Acta Medica Okayama
|
| 発行日 | 1980-12
|
| 巻 | 34巻
|
| 号 | 6号
|
| 出版者 | Okayama University Medical School
|
| 開始ページ | 409
|
| 終了ページ | 413
|
| ISSN | 0386-300X
|
| NCID | AA00508441
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
英語
|
| 論文のバージョン | publisher
|
| 査読 |
有り
|
| PubMed ID | |
| Web of Science KeyUT |
