ID | 34248 |
FullText URL | |
Author |
Sakamura, Sho
Ushijima, Takayuki
Hama, Yoichiro
Kajiura, Hiroyuki
Fujiyama, Kazuhito
Okihara, Kiyoshi
Hashimoto, Ken
Sugimoto, Hiroyuki
Yamada, Hideo
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Abstract | In a previous study (Y. Kimura et al., Biosci. Biotechnol Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Ga1 beta 1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta 1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta 1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta 1-3 linkage to beta 1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc(2)GlcNAc(2)Man(3)-GlcNAc(2)-PA as acceptor N-glycan, we detected the beta 1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta 1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
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Keywords | royal jelly glycoprotein; N-glycan
Thomsen-Friedenreich antigen (Gal beta 1-3GalNAc)
beta 1-3 galactosyltransferase
Apis mellifera
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Note | Published with permission from the copyright holder.
This is published in Bioscience Biotechnology and Biochemistry, 2007 Vol.71 Issue.4 pp.1111-1114 Publisher URL: http://dx.doi.org/10.1271/bbb.70081 Direct access to Thomson Web of Science record (C)2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
Published Date | 2007-04
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Publication Title |
Bioscience, Biotechnology, and Biochemistry
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Volume | volume71
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Issue | issue4
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Publisher | Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Publisher Alternative | 日本農芸化学会
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Start Page | 1111
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End Page | 1114
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ISSN | 0916-8451
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NCID | AA10824164
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Content Type |
Journal Article
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language |
English
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Copyright Holders | Japan Society for Bioscience, Biotechnology and Agrochemistry
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File Version | publisher
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Refereed |
True
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DOI | |
Web of Science KeyUT | |
Submission Path | biochemistry/18
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