ID | 65102 |
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Author |
Takao, Tomoka
Department of Regenerative Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Sato, Masato
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine
Fujisawa, Yuki
Department of Regenerative Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Toyoda, Eriko
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine
Yamada, Daisuke
Department of Regenerative Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Hitsumoto, Yukio
Department of Regenerative Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Nakata, Eiji
Department Orthopedic Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
ORCID
Kaken ID
Ozaki, Toshifumi
Department Orthopedic Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Kaken ID
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Takarada, Takeshi
Department of Regenerative Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
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Abstract | Background Cell sheet fabrication for articular cartilage regenerative medicine necessitates a large number of chondrocytes of consistent quality as a cell source. Previously, we have developed human-induced pluripotent stem cell (iPSC)-derived expandable -PRRX1(+) limb-bud mesenchymal cells (ExpLBM) with stable expansion and high chondrogenic capacity, while in this study; our ExpLBM technology was combined with cell sheet engineering to assess its potential as a stable cell source for articular cartilage regeneration.
Methods ExpLBM cells derived from human-induced pluripotent stem cells (hiPSCs), including 414C2 and Ff-KVs09 (HLA homozygous), were seeded onto a culture plate and two-dimensional chondrogenic induction (2-DCI) was initiated. After 2-DCI, ExpLBM-derived chondrocytes were stripped and transferred to temperature-responsive culture inserts and the chondrocyte sheets were histologically examined or transplanted into osteochondral knee defects of immunodeficient rats. Results Immunohistochemistry revealed that ExpLBM-derived cell sheets were positive for Safranin O, COL2, and ACAN but that they were negative for COL1 and RUNX2. Furthermore, the engrafted tissues in osteochondral knee defects in immunodeficient rats were stained with SafO, human VIMENTIN, ACAN, and COL2. Conclusions The present study is the first to report the chondrocyte sheet fabrication with hiPSC-derived cell source. hiPSC-derived ExpLBM would be a promising cell source for cell sheet technology in articular cartilage regenerative medicine. |
Keywords | Chondrocyte sheet
Human-induced pluripotent stem cells
Expandable limb-bud mesenchymal cells
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Published Date | 2023-02-24
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Publication Title |
Stem Cell Research and Therapy
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Volume | volume14
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Issue | issue1
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Publisher | BMC
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Start Page | 34
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ISSN | 1757-6512
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2023.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1186/s13287-023-03252-4
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License | http://creativecommons.org/licenses/by/4.0/
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Citation | Takao, T., Sato, M., Fujisawa, Y. et al. A novel chondrocyte sheet fabrication using human-induced pluripotent stem cell-derived expandable limb-bud mesenchymal cells. Stem Cell Res Ther 14, 34 (2023). https://doi.org/10.1186/s13287-023-03252-4
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 21H02643
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