Title Alternative Annual report 2009 / Research Institute for Bioresources Okayama University
FullText URL 017.pdf
Author Research Institute for Bioresources Okayama University|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2010-03-31
Volume volume17
Start Page 1
End Page 45
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Annual Report 2008 / Research Institute for Bioresources, Okayama University
FullText URL 016.pdf
Author Research Institute for Bioresources, Okayama University|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2009-03-31
Volume volume16
Start Page 1
End Page 47
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Annual Report 2007 / Research Institute for Bioresources, Okayama University
FullText URL 015.pdf
Author Research Institute for Bioresources, Okayama University|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2008-03-31
Volume volume15
Start Page 1
End Page 43
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Annual Report 2006 / Research Institute for Bioresources, Okayama University
FullText URL 014.pdf
Author Research Institute for Bioresources, Okayama University|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2007-03-31
Volume volume14
Start Page 1
End Page 50
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 013.pdf
Author Research Institute for Bioresources, Okayama University|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2005
Volume volume13
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 012.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2004
Volume volume12
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 011.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2003
Volume volume11
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 010.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2002
Volume volume10
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 009.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2001
Volume volume9
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 008.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 2000
Volume volume8
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Influence of climatic factors on "crop situation index" of wheat and barley in Okayama prefecture
FullText URL 006_001_013_019.pdf
Author Kimura, Kazuyoshi| Tanakamaru, Shigemi|
Abstract The influences of air temperature, precipitation, number of rainy days and sunshine duration on the crop situation index of wheat, six-rowed barley and two-rowed barley in Okayama prefecture were analyzed by using the data for the 30-year-period of 1966-1995. The crop situation index of these three crops showed high negative correlation with the precipitation in April and a positive correlation with sunshine duration in April. Precipitation over 150 mm or lesser sunshine duration under 190 hrs in April seems to reduce the yield of wheat and barley in Okayama.
Keywords Wheat Barley Crop situation index Precipitaion Climatic factor
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 13
End Page 19
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative エビネから分離されたインゲンマメ黄斑モザイクウイルスの諸性質
FullText URL 006_001_043_051.pdf
Author Matsumoto, Jun-Ichi| Maeda, Takanori| Inouye, Narinobu|
Abstract Bean yellow mosaic virus (BYMV) was isolated from Calanthe sp.showing mild chlorosis on the leaves, collected in Yamaguchi Prefecture in Japan. The virus was transmitted by the aphid Myzus persicae in a non-persistent manner, and by sap-inoculation to 29 out of 46 plant species from 9 out of 12 families tested. Stability in crude sap, morphology of virus particles, shape of cylidrical inclusions and the presence of cytoplasmic crystalline inclusions in the infected cells were similar to those of BYMV isolates previously reported. The virus contained a single protein species with a molecular weight of 35,000. In a microprecipition test and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the virus showed a close serological relationship to isolates of BYMV from both crocus and gladiolus, and showed a distant relationship to clover yellow vein virus. Three BYMV isolates used in this study were found to be serologically related to each other, but the virus was more closely related to the BYMV crocus isolate than to gladiolus isolate.
Keywords Calanthe sp. bean yellow mosaic virus clover yellow vein virus serological relationship
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 43
End Page 51
ISSN 0916-930X
language English
File Version publisher
Title Alternative ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング
FullText URL 006_001_053_064.pdf
Author Murata, Minoru|
Abstract Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (Ⅰ-Ⅵ) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class Ⅰ, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes.
Keywords Cytoplasm substitution line Differential screening Midget chromosome Rye Wheat
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 53
End Page 64
ISSN 0916-930X
language English
File Version publisher
Title Alternative QTL Mapping for Water Sensitivity in Barley Seeds
FullText URL 006_001_021_028.pdf
Author Iwasa, Tomohiko| Takahashi, Hidekazu| Takeda, Kazuyoshi|
Abstract Sensitivity to water condition during germination is an important character in malting barley because it affects malt poduction. Water sensitivity in malting barley is defined as the defference in germination percentage between the seeds in standard and excess-water conditions. Usually standard and excess-water condition means 4 and 8 ml water in a 90 mm perti dish, respectively. Takeda and Fukuyama (1983) examined more than 4,000 barley accessions and found 0~100% water sensitivity. This means complete to no suppression of germination by an excess-water condition. In this study, quantiative trait loci (QTL) analysis was applied to analize the inheritance of water sensitivity. Two sets of doubled haploid (DH) populations derived from Steptoe×Morex (S/M) and Harrington×TR306 (H/T) crosses,were grown in the experiment field of the Research Institute for Bioresources, Okayama University. Bulbosum method was adapted to develop DH lines (Chen and Hayes 1989). Water sensitivities of 150 (S/M) and (H/T) lines and their parents were evaluated using 50 seeds each and the germination test (25℃, 4 days) was repeated four times. Water sensitivity showed a large variation (Fig.1) and the heritability of the trait was 0.78 and 0.72 in S/M and H/T, respectively, indicating that about three-quarters of the phenotypic variance was genetic in origin. Three (S/M) and one (H/T) significant QTL were detected and 23% (S/M) and 25% (H/T) of the phenotypic variance was explained by the QTL. These QTL were located on chromosomes 2H,3H,6H and 7H (Table 1, Fig. 2). QTL relating to germination traits were lacated near the QTL controlling water sensitivity (Fig. 3). Interaction among the QTL was small (Table 2). Marker-assisted selection was effective (Table 3).
Keywords barley water sensitivity QTL analysis
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 21
End Page 28
ISSN 0916-930X
language Japanese
File Version publisher
FullText URL 007.pdf
Author 岡山大学資源生物科学研究所|
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume7
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative The Characteristics of Trace Metal Distribution of Artificial and Natural-Origin Suspended Particulate Matter (SPM) by grain size
FullText URL 006_001_029_041.pdf
Author Muramoto, Shigeki| Maitani, Toshihiko| Hiraoka, Naoko| Aoyama, Isao|
Abstract Air pollution caused by suspended particulate matter (SPM) has increased remarkedly in industrial areas, and this has become an emergency issue in the global environment. Atomospheric SPM influences the local ecosystem including various plants and animals. It is necessary to examine the distribution characteristics of such trace metals in fine particulates (especially those with a diameter (φ) of less than 2.1μm). The Environment Agency of the Japanese goverment will complete analysis of the a concentration of suspended particulate matter less than 2.4μm by the end of 1999, and will be examining the relationship between the concentration of suspended particulate matter (0.1-9.0μm) with consideration given to the particle size throughout two years. It became clear that the metals of artificial origin such as Pb, V, Cd, Ni, Cr, existed in fine particulates (0.1 or more 2.0μm). By contrast, the metals of natural origin, such as Ti, Mn, Mg, Sr, mainly existed in coarse Area in Japan.
Keywords Atomospheric Suspended particulate matter (SPM) Natural-origin metal Artificial-origin metal Biological effects
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 29
End Page 41
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Estimation of Turbulent Fluxes by Gradient Method with Surface Temperatures of Rice Plant Canopy
FullText URL 006_001_001_011.pdf
Author Miyashita, Koichi| Maitani, Toshihiko|
Abstract Micrometeorological observations including turbulent fluxes and canopy surface temperatures were made in paddy in the summer of 1997. The sensible and latent heat fluxes were estimated by the gradient method under the assumption of similarity between the turbulent exchange coefficient for momentum and that for heat and water vapor. The surface temperatures measured by a thermal imager and water vapor pressures estimated from surface temperatures and relative humidity were used for temperature and water vapor at the height(d+z0) of U=0, where d is the displacement height, z0 the roughness length and U the mean wind velocity. The sensible and latent heat fluxes estimated by the gradient method depicted typical diurnal varietions. The turbulent fluxes by the present method and eddy correlation method showed fairly good agreement, especially for latent heat fluxes. These results confirmed that this method is a promising one.
Keywords Surface temperature Rice plant canopy Thermal imager Turbulent fluxes
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1999
Volume volume6
Issue issue1
Start Page 1
End Page 11
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Habenaria Mosaic Virus, A New Member of Potyvirus from Habenaria radiata Orchid
FullText URL 005_002_155_168.pdf
Author Inouye, Narinobu| Maeda, Takanori| Mitsuhata, Koji| Gara, I Wayan|
Abstract A potyvirus, isolated from Habenaria radiaata showing severe mosaic in the leaves named habenaria mosaic virus(HaMV). HaMV was transmitted by inoculation of sap to 6 of 41 species from 4 of 12 families. Systemic infection was cauded in Habenaria radiata, and local infection was produced in Chenopodium amaranticolor, C. quinoa, Spinacia olracea, Tetragonia expansa and Gomphrena globosa. HaMV was also transmitted by Myzus persicae in a non persistent manner, but not by Aphis gossypii. The virus has filamentous particles of c. 750×13nm. Sap from inoculated C. quinoa was infective after dilution to 10-5 but not 10-6, after 10 min at 60℃ but 65℃, or after 8-16 days at 20℃.In ultrathin sections of diseased leaves of Habenaria radiata and inoculated C. quinoa, virus particles were found to disperse in the cytoplasm. Cylindrical inclusions were present in the cytoplasm of infected cells, as pinwheels, scrolls or tubes. The inclusions observed in negatively stained crude sap preparations exhibited fine linear striation at about 5.2 nm intervals.The antiserum to HaMV produced by immunizing a rabbit had a titer of 1/512(precipitin test). HaMV showed distant serological relationships to potyvirus;alstroemeria mosaic virus, bean yellow mosaic virus, calanthe mild mosaic virus, clover yellow vein virus, dendrobium mosaic virus, freesia mosaic virus, turnip mosaic virus and watermelon mosaic virus 2.
Keywords Habenaria orchid Habenaria mosaic potyvirus Habenaria mosaic disease
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 155
End Page 168
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative イネ幼植物から調整した細胞壁に含まれるペクチン質の性状
FullText URL 005_002_135_144.pdf
Author Konno, Haruyoshi| Tsumuki, Hisaaki| Yamasaki, Yoshiki|
Abstract Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4~0.6% of the starch-free cell walls.
Keywords Cell wall Oryza sativa Pectic polymer
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 135
End Page 144
ISSN 0916-930X
language English
File Version publisher
Title Alternative シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現
FullText URL 005_002_145_153.pdf
Author Sugimoto, Manabu| Kawai, Fusako|
Abstract A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one.
Keywords Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 145
End Page 153
ISSN 0916-930X
language English
File Version publisher