| Title Alternative | タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響 |
|---|---|
| FullText URL | 002_002_181_190.pdf |
| Author | Yamamoto, Yoko| Chang, Yi-Chieh| Ono, Kanji| Matsumoto, Hideaki| |
| Abstract | The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(Ⅱ)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(Ⅱ) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(Ⅱ) and Cd and sensitive to Cu. When cells were treated with both Fe(Ⅱ)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(Ⅱ), Cu and Cd in different manners; synergistic with Fe(Ⅱ), antagonistic with Cu and apparently no effeco on Cd. |
| Keywords | Aluminum Antagonistic Nicotiana tabacum Suspension culture Synergistic |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue2 |
| Start Page | 181 |
| End Page | 190 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Varietal Variation of Earliness in a Narrow Sense and Its Significance for Adaptation in Barley |
|---|---|
| FullText URL | 002_001_023_032.pdf |
| Author | Okubo, Kazuo| Yasuda, Shozo| Takeda, Kazuyoshi| |
| Abstract | Heading time of is a complex character, which is controlled by several internal factors, namely, vernalization requirement, photoperiodic response and earliness in a narrow sense (ENS). The ecological significance of vernalization requirement and photoperiodic response has been clarified. However, the significance of ENS is not explained well. In this study, we examined the varietal variation of ENS, its geographical distribution and significance of ENS for regional adaptability in barley. The ENS was estimated from the number of days from sowing to flag-leaf emergence of sufficiently vernalized plants grown under a 24-h. photoperiod at 20℃ condition. The variation of ENS was determined from the number of leaves and leaf emergence intervals under the controlled condition. The ENS of 1,017 barley varieties ranged from 12 to 28 days. ENS was shortest in varieties from low-latitudes, while it was longer in varieties from high-latitudes and very low-latitudes. Multiple regression analysis for heading time(Y) on the ENS(X1) and the photoperiodic response(X2) was carried out using various data obtained from different experiment sites and sowing times. The multiple correlation was highly significant in all cases. It was clear that the ENS played an important role for determining heading time in the spring-sown conditions, while the photoperiodic response did in the fall-sown condition. |
| Keywords | Barley Heading time Geographica distribution Adaptability |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 23 |
| End Page | 32 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Promotion of Drying of Leaves Detached from Plants after Rainfall Exposure |
|---|---|
| FullText URL | 002_002_191_200.pdf |
| Author | Kimura, Kazuyoshi| |
| Abstract | The drying of leaves detached from 16 plants (mainly, vegetalbles) after artificial rainfall (mist)treatment was examined in a growth chamber(20℃,8 klux). Leaves detached from misted plants had a higher drying rate than those detached from non-misted plants. The promoting effect of mist on the dryint rate was increased with the increase in the duration of mist exposure. The degree of drying after mist exposure for 3 days or more was increased markedly. In almost all the plants,the weight of the leaves detached from the plants decreased to 10-20% of the initial weight 24 hours after a 5-day mist treatment, but 40-50% in cabbadge, chinese leek and welsh onion. In general, the degree of drying was greater in the young developing leaves or older leaves than in active young leaves. These findings suggested that the surface wax and cuticular of the leaf is injured by rainfall wetting, and that water discharge(transpitation) in the leaf is increased greatly. |
| Keywords | Rainfall Leaf Drying Vegetables |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue2 |
| Start Page | 191 |
| End Page | 200 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Comparison of Regenerating Ability of Calli Derived from Mature and Immature Embryos in Barley Varieties |
|---|---|
| FullText URL | 002_001_033_042.pdf |
| Author | Rikiishi, Kazuhide| Yasuda, Shozo| |
| Abstract | The callus forming ability and regenerating ability of the calli derived from mature and immature embryos of 132 barley varieties were examined. These materials were taken from a world-wide collection preserved at the Barley Germplasm Center of Okayama University. The callus forming ability varied widely according to genotype in both mature and immature embryos, but the varieties collected from Ethiopia showed low callus forming ability. Calli derived from mature embryos generally did not regenerate shoots, except for three Japanese varieties. The frequency of shoot regeneration from the calli derived from immature embryos was somewhat higher than that from those derived from mature embryos. Many of the Korean and Japanese varieties had a high shoot regenerating ability. However, few of the varieties from Ethiopia and Southwest Asia had a high shoot regenerating ability. No correlation was observed btween root regenerating ability and shoot regenerating ability of the varieties. No correlation was observed between callus proliferation and root regenerating ability between calli derived from mature and immature embryos. We could not find any difference in the shoot regenerating ability btween the two-rowed and six-rowed genotypes. |
| Keywords | Barley Tissue culture Mature embryo Immature embryo Regenerating |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 33 |
| End Page | 42 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
|---|---|
| FullText URL | 003_002_091_103.pdf |
| Author | Moritsugu, Masumi| Kawasaki, Toshio| Suzuki, Takao| |
| Abstract | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
| Keywords | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 091 |
| End Page | 103 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley |
|---|---|
| FullText URL | 002_001_043_053.pdf |
| Author | Mano, Yoshiro| Rikiishi, Kazuhide| Yasuda, Shozo| |
| Abstract | Immature embryos of 99 varieties of barley were cultured to investigate the ability of callus growth and plant regeneration. These two in vitro traits showed wide and continuous variations among the barley varieties tested. Ability of callus growth, which were evaluated by callus diameter ranged from 3.9mm to 11.2mm, and ability of plant regeneration from the calli ranged from 0% to 100%. A set of complete diallel crosses was made using six cultivars as the parents which differed in ability of callus growth and plant regeneration. The Vr/Wr graphical analysis showed that there were epistasis, or interaction among nonallelic genes for callus growth. As to ability of plant regeneration, no epistasis existed in the subdiallel without P1 (J232) which showed high specific combining ability, and it was controlled by a simple additive dominance genetic system. The mean degree of dominance(0.42) was relatively low and the broad(0.86) and narrow(0.78) sense heritabilities were high. |
| Keywords | Barley Tissue culture Plant regeneration Diallel analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 53 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Effect of Light Irradiation on the Absorption of Rubidium in Chlorella |
|---|---|
| FullText URL | 003_002_105_116.pdf |
| Author | Hachiya, Kinji| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The effect of light irradiation on the absorption of rubidium was examined in Chlorella. Rubidium absorption in Chlorella was clearly stimulated by the irradiation of light. To clarify the mechanism of light-stimulation on rubidium absorption, experiments were carried out using several metabolic inhibitors; DCMU, NaCN,DNP,CCCP and ouabain. Among the metabolic inhibitors used, DCMU had the most similar effects on the rubidium absorption and oxygen evolution under light condition. These findings suggest a close correlation between the light-stimulation on rubidium absorption and the photosynthetic process in Chlorella. |
| Keywords | Chlorella Light-stimulation Metabolic inhibitors Oxygen evolution Rubidium absorption |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 116 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Inheritance of Phenol Reaction in the Awn of Barley |
|---|---|
| FullText URL | 002_001_055_062.pdf |
| Author | Takeda, Kazuyoshi| Zhang, Chan Lin| Kanatani, Ryoichi| |
| Abstract | The inheritance and geographical distribution of the phenol reaction in rice has been investigated intensively. On the other hand, in the case of barley, almost all of the varieties show positive reaction to phenol, and inheritance study of the trait has not yet been reported. We investigated the phenol reaction of ca. 5,000 barley varieties preserved at the Barley Germplasm Center of Okayama University to find about 30 varieties which show a negative reaction to the phenol. These varieties are mainly from Southwest Asia. The reaction of awn was sharpest at all parts of the plant including seeds. The positive reaction must be the prototype of barley, because Hordeum spontaneum, a possible ancestor of the cultivated barley, shows positive reaction. Inheritance and linkage studies were conducted using varieties with negative reaction and linkage testers with positive reaction. Phenol reaction was dominant and showed a clear 3:1 segregation ratio in the F2 populations, indicating the trait was controlled by a single dominant gene Ph(phenol reaction). Linkage study revealed that Ph was linked with four marker genes(three loci) on the chromosome 2 and independent of 13 other marker genes located on the chromosomes except chromosome 2. Ph may be a useful marker gene and an interesting material for molecular-biological studies. |
| Keywords | Barley Phenol reaction Linkage analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 55 |
| End Page | 62 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
|---|---|
| FullText URL | 003_002_117_127.pdf |
| Author | Yonetani, Tsutomu| Moritsugu, Masumi| Kawasaki, Toshio| |
| Abstract | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
| Keywords | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 117 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Genetic Analysis of Large Trichome in Barley Leaf Blade |
|---|---|
| FullText URL | 002_001_063_068.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | The inheritance and linkage relationship of a new hairiness trait "large trichome" was investigated in barley. Although the size of large trichome is about four times that of normal one, the character can not be recognized with the naked eye. However, it is easily identified by the roughness of leaf touch. The large trichomes develop on both sides of the leaf blades. The direction of trichome is both acropetal and basipetal. It is clearly distinguished from the extremely long trichome controlled by Pub gene. About 2,300 varieties of our Barley Germplasm Center were screened by the leaf touch to find nine varieties with large trichome. Two of them were six-rowed local variety from Pakistan, and other seven were two-rowed varieties from Europe and Japan. All of them were hulled type. Crosses of six large trichome varieties with a normal Japanese variety resulted in the large trichome type F1s, suggesting the dominant nature of the trait. The large trichome line Hokuiku 17 was crossed with various linkage testres to study the mode of inheritance and the linkage relationship of the gene. In the F2 populations, the large trichome was controlled by a single dominant gene named Ltr (large trichome), which was independentiy inherited from the following marker genes; br and gl-5 on chromosome 1; li and ν on chromosome 2; uz on chromosome 3; K and gl-3 on chromosome 4; trd on chromosome 5; ο on chromosome 6. On the other hand, from the cross between Hokuiku 17 and OUL166, Ltr was found to be linked with s and fs on chromosome 7. Although the allelism test has not been completed, the very low frequency of the large trichome type (9/2,300) indicates that the variant resulted from a recent mutation event, or the fitness of the variant is low in the natural and/or artificial selection. |
| Keywords | Barley Trichome Linkage analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 63 |
| End Page | 68 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313663 |
| Title Alternative | Effects of Mannosen on Hydroponically Grown Barley |
|---|---|
| FullText URL | 003_002_129_135.pdf |
| Author | Shibasaka, Mineo| Miyata, Masahiko| Akiyama, Yoshiko| Kawasaki, Toshio| |
| Abstract | To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. |
| Keywords | Barley Hydroponics Mannose Potassium Phosphate |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 129 |
| End Page | 135 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification |
|---|---|
| FullText URL | 003_002_137_143.pdf |
| Author | Akiyama, Yoshiko| Shibasaka, Mineo| Kawasaki, Toshio| |
| Abstract | Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged. |
| Keywords | Adenylate extraction ADP AMP ATP Barley roots |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 137 |
| End Page | 143 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum |
|---|---|
| FullText URL | 003_002_145_149.pdf |
| Author | Katsuhara, Maki| Bohnert, Hans J.| |
| Abstract | Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists. |
| Keywords | Mesembryanthemum crystallinum Potassium channel RT-PCR |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 145 |
| End Page | 149 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Character Expression and Inheritance of a "Short Upper Leaves" Mutant in Barley |
|---|---|
| FullText URL | 002_001_069_078.pdf |
| Author | Kanatani, Ryoichi| Wu, Jiri| Takeda, Kazuyoshi| |
| Abstract | A mutant which devolops an extremely shortened flag leaf and shorter upper leaves was obtained from the progeny of 90 KR of gamma-ray irradiated Fuji Nijo, a malting barley variety. This mutant was controlled by a single partial dominant gene named Sul (short upper leaves). The character expression and the inheritance of the gene were investigated in this study. Sul shortened the flag leaf and upper several leaves and uppermost internode length, while it did not affect the length of spike, lower leaves and the second or lower internodes. A reciprocal translocation of the chromosomes was observed in this mutant line, and the Sull gene was linked with the V (two-rowed) gene on chromosome 2, and also linked with the breaking point of the reciprocal translocation. Small leaf area caused by the Sull gene might reduce the mutual shading and the transpiration of the leaf canopy at the later growth stages. |
| Keywords | Barley Plant type Linkage analysis Developmental pattern |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 69 |
| End Page | 78 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Host Range and Some Properties of Orchid Fleck Virus Isolated form Oriental Cymbidium in Japan |
|---|---|
| FullText URL | 003_002_151_161.pdf |
| Author | Kondo, Hideki| Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | Orchid flck virus (OFV) was isolated from Oriental Cymbidium (Cymbidium sp.),showing chlorotic flecks on leaves. The virus was transmitted mechanically to Chenopodium quinoa,C.murale and Beta vulgaris by sap-inoculation and caused systemic infection. Local lesions were produced on C.amaranticolar, Petunia hybrida, Tetragonia expansa and Vigna sinensis. Sap from infected T.expansa was still infective after 10 mim at 40℃ but not after 10 min at 45℃, at a dilution of 10-3 but not 10-4, and after 30 min at room temperature but not after 60 min. The isolate of OFV had non-enveloped, bullet-shaped patricles measuring about 40×120－150 nm in dip preparations. However, bacilliform particles about 40×120-140 nm were observed in ultrathin sections. In ultrahtin sections of virus-infected tissues, virus patricles were detected both in the nuclei and in the cytoplasm. Moreover, the inclusions of low electron density(viroplasm) were also observed in the nuclei.Virus particles were found to attach at one end to the inner nuclear membrane. A number of particles surrounded by the inner membrane often showed an appearance like a spoked wheel. |
| Keywords | Orchid fleck virus Oriental Cymbidium Non-enveloped rhabdovirus like particles |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 151 |
| End Page | 161 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313940 |
| Title Alternative | Diallel Analysis for the Percentage of Grains with Hull Rupture in F2 Populations of Two-rowed Barley |
|---|---|
| FullText URL | 002_001_079_089.pdf |
| Author | Wu, Jiri| Takeda, Kazuyoshi| Kanatani, Ryoichi| |
| Abstract | Half diallel F2 populations crossed among eight two-rowed barley varieties with warious percentages of hull ruptured grains were raised to analyze the inheritance of the trait. The percentage of grains with hull rupture varied from 0 to 87% among the parents. The diallel analysis revealed the following: the trait was predominantly controlled by the additive genes, and the dominance effect of some parents was also significant. However, the epistatic effect of the genes was not significant. The average dominance was 0.97. The heritability value was estimated as 0.57 and 0.91 in a narrow and a broad sense, respectivelly. The percentage of grains with hull rupture showed continuous and transgressive segregations in 28 F2 populations derived from half diallel crosses among eight parents. Heritability of the trait in a broad sense was 0.43~0.80(0.65 on average) in 28 F2 populations. |
| Keywords | Barley Grain quality Hull rupture Diallel analysis Heritability |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 79 |
| End Page | 89 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Sources of Resistance to Net Blotch in Barley Germplasm |
|---|---|
| FullText URL | 002_001_091_102.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
| Keywords | Barley Net blotch Disease resistance Genetic resources Race differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 91 |
| End Page | 102 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313965 |
| Title Alternative | Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan |
|---|---|
| FullText URL | 003_002_163_174.pdf |
| Author | Matsumoto, Jun-ichi| Maeda, Takanori| Inouye, Narinobu| |
| Abstract | A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. |
| Keywords | Odontoglossum ringspot virus Calanthe sp. Identification |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue2 |
| Start Page | 163 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | インドネシアのランに発生するウイルスの検索と同定 |
|---|---|
| FullText URL | 004_002_109_118.pdf |
| Author | Inouye, Narinobu| Gara, I Wayan| |
| Abstract | Three viruses, Cymbidium mosaic virsu(CyMV), odontoglossum ringspot virus (ORSV) and an unidentified potyvirus were found in the orchids in Indonesia. CyMV was detected from orchids in 8 genera, namely Aranthera, Calanthe, Cattleya, Cymbidium, Gromatophyllum, Phalaenopsis,Oncidium and Vanda. The virus was widespread in many orchids in Indonesia and was common in Aranthera and Calanthe, thus being an economically important virus in Indonesia.ORSV was also detected in orchids of 5 genera, namely Bulvophyllum,Calanthe, Cattleya,Oncidium and Phalaenopsis. The unidentified potyvirus was found in Aranthera. |
| Keywords | Cymbidium mosaic virus Odontoglossum ringspot virus Unidentified potyvirus Identification Orchids in Indonesia |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 109 |
| End Page | 118 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | 大麦うどんこ病菌(Erysiphe graminis DC. f. sp. hordei)の地理的分化 |
|---|---|
| FullText URL | 002_001_103_110.pdf |
| Author | Konishi, Takeo| Heta, Hideo| |
| Abstract | Fifteen cultures of barley powdery mildew fungus (Erysiphe graminis DC. f. sp. hordei) isolated in different regions of the world were inoculated to 120 barley varieties. Infection scores varied among the cultures and barley varieties, and their interactions were observed. Principal component analysis of the infection scores revealed that the cultures could be classified into three groups by first and second components (contributing 40.2% and 15.6% of the total variance, respectively), and that two Japanese cultures could be distinguished from the others isolated in Europe and North America. This indicates that the fungus is geographically differentiated in the reaction of the barley varieties to the cultures. Furthermore, East Asian barley varieties differed from European ones in their reactions to the culltures, while barley varieties from regions between East Asia and Europe showed a large genetic diversity in their reactions. |
| Keywords | Barley Powdery mildew Geographic distribution Differentiation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1994 |
| Volume | volume2 |
| Issue | issue1 |
| Start Page | 103 |
| End Page | 110 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
