ID | 13793 |
Eprint ID | 13793
|
FullText URL | |
Title Alternative | Sustaining capacity of hemopoiesis by human stromal cells in bone marrow
|
Author |
Ueki, Koubun
|
Abstract | A bone marrow coculturing system developed by M. Y.Gordon was modified for easier evaluation of the function of bone marrow stromal cells. In the method agar was not used, and cells were dried and stained directly on a 35-mm petri dish after culture. The sustaining capacity of hemopoiesis by human stromal cells in bone marrow obtained by this method was compared between eight young and five eldery healthy volunteers. Hemopoietic progenitor cells were obtained from young volunteers and cultures were prepared for seven days. These appeared 86±28 colonies from 10(5) non-adherent bone marrow cells on the sheet of stromal cells from the young volunteers and 89±27 colonies from the eldery. The colonies were classified into two types according to cytochemical appearance through peroxidase staining, but all of them proved to be composed of myeloid cells by enzyme-labeled antibody staining. Colonies were identified easily and accurately by the newly modified coculturing method. No difference in sustaining capacity of hemopoiesis by human stromal cells in bone marrow was found between the young and the eldery using this system.
|
Keywords | human marrow stromal cell
ability to sustain hemopoiesis
IL-6 production
aging
|
Note | 原著
|
Published Date | 1994-08
|
Publication Title |
岡山医学会雑誌
|
Publication Title Alternative | Journal of Okayama Medical Association
|
Volume | volume106
|
Issue | issue7-8
|
Publisher | 岡山医学会
|
Publisher Alternative | Okayama Medical Association
|
Start Page | 837
|
End Page | 846
|
ISSN | 0030-1558
|
NCID | AN00032489
|
Content Type |
Journal Article
|
Official Url | https://www.jstage.jst.go.jp/article/joma1947/106/7-8/106_7-8_837/_article/-char/ja/
|
Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
|
language |
Japanese
|
Copyright Holders | 岡山医学会
|
File Version | publisher
|
Refereed |
True
|
Eprints Journal Name | joma
|