ID | 13793 |
Eprint ID | 13793
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フルテキストURL | |
タイトル(別表記) | Sustaining capacity of hemopoiesis by human stromal cells in bone marrow
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著者 |
植木 康文
岡山大学医学部第二内科学教室
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抄録 | A bone marrow coculturing system developed by M. Y.Gordon was modified for easier evaluation of the function of bone marrow stromal cells. In the method agar was not used, and cells were dried and stained directly on a 35-mm petri dish after culture. The sustaining capacity of hemopoiesis by human stromal cells in bone marrow obtained by this method was compared between eight young and five eldery healthy volunteers. Hemopoietic progenitor cells were obtained from young volunteers and cultures were prepared for seven days. These appeared 86±28 colonies from 10(5) non-adherent bone marrow cells on the sheet of stromal cells from the young volunteers and 89±27 colonies from the eldery. The colonies were classified into two types according to cytochemical appearance through peroxidase staining, but all of them proved to be composed of myeloid cells by enzyme-labeled antibody staining. Colonies were identified easily and accurately by the newly modified coculturing method. No difference in sustaining capacity of hemopoiesis by human stromal cells in bone marrow was found between the young and the eldery using this system.
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キーワード | human marrow stromal cell
ability to sustain hemopoiesis
IL-6 production
aging
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備考 | 原著
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発行日 | 1994-08
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 106巻
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号 | 7-8号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 837
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終了ページ | 846
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/106/7-8/106_7-8_837/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/index.html
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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