ID | 56687 |
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Author |
Imamura, Daisuke
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Mizuno, Tamaki
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Miyoshi, Shin‐ichi
Shinoda, Sumio
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
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Abstract | Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
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Keywords | Vibrio cholerae
resuscitation
viable but nonculturable
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Note | This is an Accepted Manuscript of an article published by Wiley-Blackwell
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Published Date | 2015-02-09
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Publication Title |
Microbiology and immunology
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Volume | volume59
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Issue | issue5
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Publisher | Wiley-Blackwell
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Start Page | 305
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End Page | 310
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ISSN | 03855600
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NCID | AA00738350
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.1111/1348-0421.12246
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Project |
Collaborative Research of Okayama University for Infectious Diseases in India
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