ID | 56687 |
フルテキストURL | |
著者 |
Imamura, Daisuke
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Mizuno, Tamaki
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
Miyoshi, Shin‐ichi
Shinoda, Sumio
Collaborative Research Center of Okayama University for Infectious Diseases in India, National Institute of Cholera and Enteric Diseases
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抄録 | Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
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キーワード | Vibrio cholerae
resuscitation
viable but nonculturable
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備考 | This is an Accepted Manuscript of an article published by Wiley-Blackwell
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発行日 | 2015-02-09
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出版物タイトル |
Microbiology and immunology
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巻 | 59巻
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号 | 5号
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出版者 | Wiley-Blackwell
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開始ページ | 305
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終了ページ | 310
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ISSN | 03855600
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NCID | AA00738350
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1111/1348-0421.12246
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プロジェクト |
インド感染症共同研究
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