JaLCDOI 10.18926/AMO/32819
FullText URL fulltext.pdf
Author Okamoto, Osamu| Yamamoto, Yuji| Inagaki, Sachiyo| Yoshitome, Kei| ishikawa, Takaki| Imabayashi, Kiyomi| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>Allele and genotype frequencies for 15 short tandem repeat (STR) polymorphisms--D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA--in a Japanese population were estimated. No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for any of the systems studied. Between 2 new pentanucleotide STR loci, Penta E and Penta D, for which there is only limited data regarding the allelic distribution in Japanese, the Penta E locus was found to be highly polymorphic and exhibited a tri- or tetra-modal distribution pattern having allelic peaks with 5, 11, 15 and 20 repeats. The distribution was significantly different from that of the other ethnic groups. Statistical parameters of forensic importance, the power of discrimination (PD), observed and expected heterozygosity values (H), polymorphism information content (PIC), power of discrimination (PD), matching probability (pM), power of exclusion (PE), and typical paternity index (PI), were calculated for the loci. These parameters indicated the usefulness of the loci in forensic personal identification and paternity testing among Japanese. The systems Penta E, FGA, D18S51 and D8S1179 were the most informative. This method was successfully applied to forensic personal identification and paternity testing among Japanese, thereby confirming its efficacy for forensic practice.</p>
Keywords population data DNA typing short tandem repests personal identification paternity testing
Amo Type Article
Published Date 2003-04
Publication Title Acta Medica Okayama
Volume volume57
Issue issue2
Publisher Okayama University Medical School
Start Page 59
End Page 71
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12866745
Web of Sience KeyUT 000182520400003
JaLCDOI 10.18926/AMO/32309
FullText URL fulltext.pdf
Author Murakami, Hiroki| Ymamamoto, Yuji| Yoshitome, Kei| Ono, Toshiaki| Okamoto, Osamu| Shigeta, Yoshiaki| Doi, Yusuke| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>In this study, sex determination using polymerase chain reaction (PCR) on tooth material was evaluated from the viewpoint of forensic medicine. The sensitivity of PCR for detection of the Y chromosome-specific alphoid repeat sequence and the X chromosome-specific alphoid repeat sequence was 0.5 pg of genomic DNA. Sex could be determined by PCR of DNA extracted from the pulp of 16 freshly extracted permanent teeth and dentine including the surface of the pulp cavity of 6 freshly extracted milk teeth. Sex could be determined using the pulp in all 20 teeth (10 male and 10 female) preserved at room temperature for 22 years. For the pulp of teeth stored in sea water, the sex could be determined in all 8 teeth immersed for 1 week and in 5 of 6 teeth immersed for 4 weeks. In the remaining 1 tooth, in which sex determination based on the pulp failed, the sex could be determined correctly when DNA extracted from the tooth hard tissue was examined. For teeth stored in soil, the sex could be determined accurately in all 8 teeth buried for 1 week, 7 of 8 teeth buried for 4 weeks, and in all 6 teeth buried for 8 weeks. When teeth were heated for 30 min, sex determination from the pulp was possible in all teeth heated to 100, 150, and 200 degrees C, and even in some teeth heated to 250 degrees C. When this method was applied to actual forensic cases, the sex of a mummified body estimated to have been discovered half a year to 1 year after death could be determined readily by examination of the dental pulp. In the skeletons of 2 bodies placed under water for approximately 1 year and approximately 11 years and 7 months, pulp tissues had been dissolved and lost, but sex determination was possible using DNA extracted from hard dental tissues. These results indicate that this method is useful in forensic practices for sex determination based on teeth samples.</p>
Keywords personal identification sex determination tooth deoxyribonucleic acid (DNA). polymerase chain reaction
Amo Type Article
Published Date 2000-02
Publication Title Acta Medica Okayama
Volume volume54
Issue issue1
Publisher Okayama University Medical School
Start Page 21
End Page 32
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10709619
Web of Sience KeyUT 000085526000004
JaLCDOI 10.18926/AMO/32024
FullText URL fulltext.pdf
Author Ono, Toshiaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Ishikawa, Takaki| Ishizu, Hideo|
Abstract <p>We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.&#60;/P&#62;</p>
Keywords species identification myoglobin polymerase chain reaction
Amo Type Article
Published Date 2001-06
Publication Title Acta Medica Okayama
Volume volume55
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 184
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11434430
Web of Sience KeyUT 000169512600004
JaLCDOI 10.18926/AMO/31971
FullText URL fulltext.pdf
Author Imabayashi, Kiyomi| Yamamoto, Yuji| Inagaki, Sachiyo| Doi, Yusuke| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.</p>
Keywords HLA-DRB1 genotyping group specific primer single nucleotide polymorphism multiplex primer extension reactions application to mixed samples
Amo Type Article
Published Date 2005-10
Publication Title Acta Medica Okayama
Volume volume59
Issue issue5
Publisher Okayama University Medical School
Start Page 179
End Page 194
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16286957
Web of Sience KeyUT 000232835600002
JaLCDOI 10.18926/AMO/31815
FullText URL fulltext.pdf
Author Kamizato, Eigo| Yoshitome, Kei| Yamamoto, Yuji| Iwase, Toshihide| Tsuda, Toshihide| Miyaishi, Satoru| Doi, Hiroyuki|
Abstract <p>The annual number of suicides in Japan increased sharply in 1998, and since that time it has consistently exceeded 30,000 per year. In this study, we analyze a database of personal and background characteristics of 824 cases (605 men, 219 women) who completed suicide in Okayama Prefecture in 2002 and 2003. The data were obtained with cooperation from the police. Using the methodologies in a previous European study as a model, we classified the suicide methods into 8 categories. To examine the generational and regional differences in the choice of methods, we stratified the sample into 4 age groups (&#60;-24, 2544, 4564, and &#62;-65) and 2 regional groups (Okayama/Kurashiki vs. other areas). Our results on gender differences in 7 of the suicide methods were mostly similar to the European data. However, our data showed a remarkably higher proportionate male-to-female mortality ratio for poisoning by other substances (ICD-10, X65-X69 codes) (1.83, 1.15-2.92). In terms of generational differences in the choice of suicide methods, the Mantel-Haenszel test of homogeneity was significant for most of the categories in our study, suggesting an impact of age on how people commit suicide. There were no remarkable regional differences in our sample. An epidemic curve for suicides via carbon monoxide poisoning using charcoal briquets revealed a trend of time clustering not observed in the other 6 means. The database constructed and used in this study contains richer information than conventional death statistics and is expected to provide helpful knowledge and insights for future epidemiological studies.</p>
Keywords suicide methods gender-specific legal medicine cluster suicide
Amo Type Original Article
Published Date 2009-08
Publication Title Acta Medica Okayama
Volume volume63
Issue issue4
Publisher Okayama University Medical School
Start Page 177
End Page 186
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19727202
Web of Sience KeyUT 000269228400003
JaLCDOI 10.18926/AMO/31726
FullText URL fulltext.pdf
Author Yoshitome, Kei| Ishikawa, Takaki| Inagaki, Sachiyo| Yamamoto, Yuji| Miyaishi, Satoru| Ishizu, Hideo|
Abstract <p>We encountered a rare case of suffocation by an advertising balloon filled with pure helium gas. Suffocation caused by inhalation of atmosphere lacking in oxygen is not exceptional, but reports of death by suffocation due to a pure inert gas such as helium are very rare. In this case, the balloon mooring on the ground was enclosed, warning signs were displayed, and it was clear that entering the balloon filled with an atmosphere lacking in oxygen was extremely dangerous and should not be done; the accident did, however, occur. Accidents of this kind may occur in the future unless appropriate education and countermeasures are taken.</p>
Keywords asphyxia suffocation helium advertising balloon atmosphere lacking in oxygen
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 53
End Page 55
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873946
Web of Sience KeyUT 000174031300010
Author Ishikawa, Takaki| Miyaishi, Satoru| Yamamoto, Yuji| Yoshitome, Kei| Inagaki, Sachiyo| Okamura, Michihiko| Ishizu, Hideo|
Published Date 2001-12-31
Publication Title 岡山医学会雑誌
Volume volume113
Issue issue3
Content Type Journal Article
Author Ishikawa, Takaki| Miyaishi, Satoru| Doi, Yusuke| Takata, Tomoyo| Imabayashi, Kiyomi| Inagaki, Sachiyo| Yoshitome, Kei| Yamamoto, Yuji| Ishizu, Hideo|
Published Date 2003-01-31
Publication Title 岡山医学会雑誌
Volume volume114
Issue issue3
Content Type Journal Article
Author Ishikawa, Takaki| Furusato, Masakuni| Yoshitome, Kei| Miyaishi, Satoru| Ishizu, Hideo|
Published Date 2005-05-30
Publication Title 岡山医学会雑誌
Volume volume115
Issue issue1
Content Type Journal Article