ID | 61197 |
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Takabatake, Kiyofumi
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
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Tsujigiwa, Hidetsugu
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
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Nakano, Keisuke
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
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Inada, Yasunori
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Qiusheng, Shan
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Kawai, Hotaka
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Sukegawa, Shintaro
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
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Fushimi, Shigeko
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Nagatsuka, Hitoshi
Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
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Abstract | Recently, dental pulp has been attracting attention as a promising source of multipotent mesenchymal stem cells (MSCs) for various clinical applications of regeneration fields. To date, we have succeeded in establishing rat dental pulp-derived cells showing the characteristics of odontoblasts under in vitro conditions. We named them Tooth matrix-forming, GFP rat-derived Cells (TGC). However, though TGC form massive dentin-like hard tissues under in vivo conditions, this does not lead to the induction of polar odontoblasts. Focusing on the importance of the geometrical structure of an artificial biomaterial to induce cell differentiation and hard tissue formation, we previously have succeeded in developing a new biomaterial, honeycomb tricalcium phosphate (TCP) scaffold with through-holes of various diameters. In this study, to induce polar odontoblasts, TGC were induced to form odontoblasts using honeycomb TCP that had various hole diameters (75, 300, and 500 mu m) as a scaffold. The results showed that honeycomb TCP with 300-mu m hole diameters (300TCP) differentiated TGC into polar odontoblasts that were DSP positive. Therefore, our study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration.
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Keywords | dental pulp
honeycomb TCP
matrix formation
dentin formation
geometrical structure
scaffold
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Published Date | 2020-11-16
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Publication Title |
Materials
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Volume | volume13
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Issue | issue22
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Publisher | MDPI
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Start Page | 5155
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ISSN | 1996-1944
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2020 by the authors.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.3390/ma13225155
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License | http://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Society for the Promotion of Science
Japan Agency for Medical Research and Development
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助成番号 | JP19K19159
JP20K10178
JP20K23079
JP20lm0203008
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