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ID 61197
フルテキストURL
fulltext.pdf 49.6 MB
著者
Takabatake, Kiyofumi Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University Kaken ID publons researchmap
Tsujigiwa, Hidetsugu Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University Kaken ID publons researchmap
Nakano, Keisuke Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University ORCID Kaken ID publons researchmap
Inada, Yasunori Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Qiusheng, Shan Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Kawai, Hotaka Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Sukegawa, Shintaro Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University ORCID Kaken ID publons
Fushimi, Shigeko Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University
Nagatsuka, Hitoshi Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University Kaken ID publons researchmap
抄録
Recently, dental pulp has been attracting attention as a promising source of multipotent mesenchymal stem cells (MSCs) for various clinical applications of regeneration fields. To date, we have succeeded in establishing rat dental pulp-derived cells showing the characteristics of odontoblasts under in vitro conditions. We named them Tooth matrix-forming, GFP rat-derived Cells (TGC). However, though TGC form massive dentin-like hard tissues under in vivo conditions, this does not lead to the induction of polar odontoblasts. Focusing on the importance of the geometrical structure of an artificial biomaterial to induce cell differentiation and hard tissue formation, we previously have succeeded in developing a new biomaterial, honeycomb tricalcium phosphate (TCP) scaffold with through-holes of various diameters. In this study, to induce polar odontoblasts, TGC were induced to form odontoblasts using honeycomb TCP that had various hole diameters (75, 300, and 500 mu m) as a scaffold. The results showed that honeycomb TCP with 300-mu m hole diameters (300TCP) differentiated TGC into polar odontoblasts that were DSP positive. Therefore, our study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration.
キーワード
dental pulp
honeycomb TCP
matrix formation
dentin formation
geometrical structure
scaffold
発行日
2020-11-16
出版物タイトル
Materials
13巻
22号
出版者
MDPI
開始ページ
5155
ISSN
1996-1944
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2020 by the authors.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.3390/ma13225155
ライセンス
http://creativecommons.org/licenses/by/4.0/
助成機関名
日本学術振興会
Japan Agency for Medical Research and Development
助成番号
JP19K19159
JP20K10178
JP20K23079
JP20lm0203008