FullText URL | fulltext.pdf |
---|---|
Author | Saito, Yukihiro| Nose, Naoko| Iida, Toshihiro| Akazawa, Kaoru| Kanno, Takayuki| Fujimoto, Yuki| Sasaki, Takanori| Akehi, Masaru| Higuchi, Takahiro| Akagi, Satoshi| Yoshida, Masashi| Miyoshi, Toru| Ito, Hiroshi| Nakamura, Kazufumi| |
Keywords | sodium/iodide symporter human induced pluripotent stem cell-derived cardiomyocytes single photon emission computed tomography cell-based therapy in vivo imaging |
Published Date | 2023-09-07 |
Publication Title | Frontiers in Cardiovascular Medicine |
Volume | volume10 |
Publisher | Frontiers Media |
Start Page | 1261330 |
ISSN | 2297-055X |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2023 Saito, Nose, Iida, Akazawa, Kanno, Fujimoto, Sasaki, Akehi, Higuchi, Akagi, Yoshida, Miyoshi, Ito and Nakamura. |
File Version | publisher |
PubMed ID | 37745108 |
DOI | 10.3389/fcvm.2023.1261330 |
Web of Science KeyUT | 001069842100001 |
Related Url | isVersionOf https://doi.org/10.3389/fcvm.2023.1261330 |
FullText URL | fulltext.pdf |
---|---|
Author | Matsusaka, Yohji| Werner, Rudolf A.| Arias-Loza, Paula| Nose, Naoko| Sasaki, Takanori| Chen, Xinyu| Lapa, Constantin| Higuchi, Takahiro| |
Published Date | 2022-07-16 |
Publication Title | Molecular Imaging |
Volume | volume2022 |
Publisher | Hindawi Ltd. |
Start Page | 9810097 |
ISSN | 1536-0121 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2022 Yohji Matsusaka et al. |
File Version | publisher |
PubMed ID | 35903250 |
DOI | 10.1155/2022/9810097 |
Web of Science KeyUT | 000887057400001 |
Related Url | isVersionOf https://doi.org/10.1155/2022/9810097 |
FullText URL | fulltext.pdf |
---|---|
Author | Matsusaka, Yohji| Chen, Xinyu| Arias-Loza, Paula| Werner, Rudolf A.| Nose, Naoko| Sasaki, Takanori| Rowe, Steven P.| Pomper, Martin G.| Lapa, Constantin| Higuchi, Takahiro| |
Published Date | 2022-06-21 |
Publication Title | Molecular Imaging |
Volume | volume2022 |
Publisher | Hindawi Ltd. |
Start Page | 4635171 |
ISSN | 1536-0121 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2022 Yohji Matsusaka et al. |
File Version | publisher |
PubMed ID | 35903251 |
DOI | 10.1155/2022/4635171 |
Web of Science KeyUT | 000890332200001 |
Related Url | isVersionOf https://doi.org/10.1155/2022/4635171 |
FullText URL | fulltext.pdf |
---|---|
Author | Lim, Melissa Siaw Han| Ohtsuki, Takashi| Takenaka, Fumiaki| Kobayashi, Kazuko| Akehi, Masaru| Uji, Hirotaka| Kobuchi, Hirotsugu| Sasaki, Takanori| Ozeki, Eiichi| Matsuura, Eiji| |
Keywords | theranostics single chain variable fragment of IgG (scFv) drug delivery system (DDS) photodynamic therapy (PDT) PET imaging accelerated blood clearance (ABC) cell penetrating peptide (CPP) siRNA ATP-binding cassette subfamily G member 2 (ABCG2) |
Published Date | 2021-02-18 |
Publication Title | Life |
Volume | volume11 |
Issue | issue2 |
Publisher | MDPI |
Start Page | 158 |
ISSN | 2075-1729 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2021 by the authors. |
File Version | publisher |
PubMed ID | 33670777 |
DOI | 10.3390/life11020158 |
Web of Science KeyUT | 000622756700001 |
Related Url | isVersionOf https://doi.org/10.3390/life11020158 |
FullText URL | fulltext.pdf |
---|---|
Author | Komaki, Toshiyuki| Hiraki, Takao| Kamegawa, Tetsushi| Matsuno, Takayuki| Sakurai, Jun| Matsuura, Ryutaro| Yamaguchi, Takuya| Sasaki, Takanori| Mitsuhashi, Toshiharu| Okamoto, Soichiro| Uka, Mayu| Matsui, Yusuke| Iguchi, Toshihiro| Gobara, Hideo| Kanazawa, Susumu| |
Keywords | Robotics Interventional radiology Animal experiments |
Published Date | 2019-11-26 |
Publication Title | European Radiology |
Volume | volume30 |
Issue | issue3 |
Publisher | Springer |
Start Page | 1342 |
End Page | 1349 |
ISSN | 0938-7994 |
NCID | AA10824302 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © Authors |
File Version | publisher |
PubMed ID | 31773299 |
DOI | 10.1007/s00330-019-06477-1 |
Web of Science KeyUT | 000517458800006 |
Related Url | isVersionOf https://doi.org/10.1007/s00330-019-06477-1 |
JaLCDOI | 10.18926/AMO/53999 |
---|---|
FullText URL | 70_1_13.pdf |
Author | Arum Tri Wahyuningsih| Shen, Lianhua| Kobayashi, Kazuko| Sasaki, Takanori| Takenaka, Fumiaki| Hanada, Takahisa| Akehi, Masaru| Akahoshi, Akiya| Ozeki, Eiichi| Ando, Eiji| Matsuura, Eiji| |
Abstract | Intact β2-glycoprotein I (iβ2GPI) is a glycoprotein that regulates coagulation and fibrinolysis. Nicked β2GPI (nβ2GPI) possesses an angiogenic property at a relatively low concentration, and an antiangiogenic property at a high concentration. Here we investigated the functions of βi 2GPI and nβ2GPI in vascular endothelial growth factor (VEGF)-A-induced endothelial cell proliferation and tube formation. We used noninvasive PET imaging to analyze the in vivo distribution of intravenously injected β2GPI variants in tumor lesions in mice. iβ2GPI was incubated with plasmin to obtain nβ2GPI, and its N-terminal sequence was analyzed. nβ2GPI had at least one other cleavage site upstream of the β2GPIʼs domain V, whereas the former plasmin-cleavage site locates between K317 and T318. Both of intact and nicked β2GPI significantly inhibited the VEGF-A-induced cell proliferation and the tube formation of human umbilical vein endothelial cells (HUVECs). PET imaging visualized considerably distributed intensities of all tested β2GPI variants in tumor lesions of pancreatic tumor cell-xenografts. These results indicate that β2GPI may be physiologically and pathophysiologically important in the regulation of not only coagulation and fibrinolysis, but also angiogenesis. |
Keywords | β2-glycoprotein I (β2GPI) angiogenesis vascular endothelial growth factor-A (VEGF-A) positron emission tomography (PET) imaging |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2016-02 |
Volume | volume70 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 13 |
End Page | 24 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2016 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 26899605 |
Web of Science KeyUT | 000371288700002 |
JaLCDOI | 10.18926/AMO/52009 |
---|---|
FullText URL | 67_6_359.pdf |
Author | Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi| |
Abstract | It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis. |
Keywords | ADC apoptosis necrosis hyperthermia cell density |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2013-12 |
Volume | volume67 |
Issue | issue6 |
Publisher | Okayama University Medical School |
Start Page | 359 |
End Page | 367 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 24356720 |
Web of Science KeyUT | 000328915700004 |
JaLCDOI | 10.18926/AMO/48566 |
---|---|
FullText URL | 66_3_263.pdf |
Author | Sasaki, Takanori| Kuroda, Masahiro| Katashima, Kazunori| Ashida, Masakazu| Matsuzaki, Hidenobu| Asaumi, Junichi| Murakami, Jun| Ohno, Seiichiro| Kato, Hirokazu| Kanazawa, Susumu| |
Abstract | The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittセs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values. |
Keywords | apparent diffusion coefficient value cell density extracellular space bio-phantom |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2012-06 |
Volume | volume66 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 263 |
End Page | 270 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 22729107 |
Web of Science KeyUT | 000305669700010 |