ID | 60527 |
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Akhtar, Parveen
Biological Research Centre
Nowakowski, Paweł J.
ivision of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University
Wang, Wenda
Photosynthesis Research Centre, Chinese Academy of Sciences
Do, Thanh Nhut
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University
Zhao, Songhao
Photosynthesis Research Centre, Chinese Academy of Sciences
Siligardi, Giuliano
Diamond Light Source Ltd., Harwell Science and Innovation Campus
Garab, Győző
Biological Research Centre
Shen, Jian-Ren
Research Institute for Interdisciplinary Science, Graduate School of Natural Science and Technology, Okayama University
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Tan, Howe-Siang
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University
Lambrev, Petar H.
Biological Research Centre
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Abstract | Light-harvesting complex II (LHCII) from the marine green macroalga Bryopsis corticulans is spectroscopically characterized to understand the structural and functional changes resulting from adaptation to intertidal environment. LHCII is homologous to its counterpart in land plants but has a different carotenoid and chlorophyll (Chl) composition. This is reflected in the steady-state absorption, fluorescence, linear dichroism, circular dichroism and anisotropic circular dichroism spectra. Time-resolved fluorescence and two-dimensional electronic spectroscopy were used to investigate the consequences of this adaptive change in the pigment composition on the excited-state dynamics. The complex contains additional Chl b spectral forms – absorbing at around 650 nm and 658 nm – and lacks the red-most Chl a forms compared with higher-plant LHCII. Similar to plant LHCII, energy transfer between Chls occurs on timescales from under hundred fs (mainly from Chl b to Chl a) to several picoseconds (mainly between Chl a pools). However, the presence of long-lived, weakly coupled Chl b and Chl a states leads to slower exciton equilibration in LHCII from B. corticulans. The finding demonstrates a trade-off between the enhanced absorption of blue-green light and the excitation migration time. However, the adaptive change does not result in a significant drop in the overall photochemical efficiency of Photosystem II. These results show that LHCII is a robust adaptable system whose spectral properties can be tuned to the environment for optimal light harvesting.
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Keywords | Circular dichroism
Light-harvesting complexes
Marine algae
Photosynthesis
Time-resolved spectroscopy
Two-dimensional spectroscopy
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Published Date | 2020-07-01
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Publication Title |
Biochimica et Biophysica Acta (BBA) - Bioenergetics
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Volume | volume1861
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Issue | issue7
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Publisher | Elsevier
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Start Page | 148191
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ISSN | 0005-2728
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NCID | AA00564646
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2020 The Authors
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1016/j.bbabio.2020.148191
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License | http://creativecommons.org/licenses/by/4.0/
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Open Access (Publisher) |
OA
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Open Archive (publisher) |
Non-OpenArchive
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