JaLCDOI | 10.18926/AMO/30945 |
---|---|
FullText URL | fulltext.pdf |
Author | Kobayashi, Tomoko| Sakaguchi, Masakiyo| Tanimoto, Ryuta| Abarzua, Fernando| Takaishi, Mikiro| Kaku, Haruki| Kataoka, Ken| Saika, Takashi| Nasu, Yasutomo| Miyazaki, Masahiro| Kumon, Hiromi| Huh, Nam-ho| |
Abstract | We have recently shown that a new therapeutic modality using the REIC/Dkk-3 gene (Ad-REIC) is effective against various human cancers, including those of prostate, testis and breast origins. The aim of the present study was to examine the sensitivity of bladder cancers to Ad-REIC and to clarify the molecular mechanisms that determine sensitivity/resistance. We found that 2 human bladder cancer cell lines, T24 and J82, are resistant to Ad-REIC. In T24 and J82 cells, the ER stress response and activation of JNK were observed in a manner similar to that in the sensitive PC3 cells. Translocation of Bax to mitochondria occurred in PC3 cells but not in T24 and J82 cells. Bcl-2 was remarkably overexpressed in T24 and J82 compared with the expression levels in sensitive cell lines. Treatment of T24 and J82 cells with a Bcl-2 inhibitor sensitized the cells to Ad-REIC-induced apoptosis. The results indicate that some human bladder cancers are resistant to apoptosis induced by overexpression of REIC/Dkk-3, which is at least in part due to up-regulation of Bcl-2. These results provide a basis for possible use of Bcl-2 as a marker of sensitive cancers and to try to sensitize resistant cancers to Ad-REIC by down-regulation of Bcl-2. |
Keywords | REIC/Dkk-3 bladder cancer apoptosis Bcl-2 |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2008-12 |
Volume | volume62 |
Issue | issue6 |
Publisher | Okayama University Medical School |
Start Page | 393 |
End Page | 401 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
Web of Science KeyUT | 000262025000006 |
JaLCDOI | 10.18926/AMO/31719 |
---|---|
FullText URL | fulltext.pdf |
Author | Kondo, Asami| Sakaguchi, Masakiyo| Makino, Eiichi| Namba, Masayoshi| Okada, Shigeru| Huh, Nam-ho| |
Abstract | Using 2-dimensional gel electrophoresis, we previously demonstrated that the S100C protein remarkably decreased after immortalization of normal human fibroblasts, and that this protein caused growth inhibition of human tumor cells when forcibly expressed in these cells, suggesting that S100C plays a significant role in tumor suppression. The present study was carried out to determine what type of human tissues express S100C protein, and, subsequently, whether the S100C content in these tissues changes after normal cells have been transformed into cancer cells. We found that ductal cells in various tissues were positively stained with the S100C protein. In comparison, epithelial cells in digestive organs such as the stomach, small intestine, and colon were not stained as strongly. When 14 pairs of human normal and cancerous tissues were stained with the antibody, decreases in the staining levels of S100C were observed in 6 kinds of cancerous tissues--from the bronchus, mammary duct, renal tubule, prostate, uterus, and testis--in comparison with staining in their normal counterparts. These results suggest that S100C is a new tumor marker protein, the expression of which significantly decreases after malignant transformation of human tissues. |
Keywords | S100C-antibody human tissues immunostaining |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 2002-02 |
Volume | volume56 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 31 |
End Page | 34 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 11873942 |
Web of Science KeyUT | 000174031300006 |
JaLCDOI | 10.18926/AMO/48076 |
---|---|
FullText URL | 66_1_7.pdf |
Author | Kawauchi, Keiichiro| Watanabe, Masami| Kaku, Haruki| Huang, Peng| Sasaki, Kasumi| Sakaguchi, Masakiyo| Ochiai, Kazuhiko| Huh, Nam-ho| Nasu, Yasutomo| Kumon, Hiromi| |
Abstract | The preclinical safety and therapeutic efficacy of adenoviral vectors that express the REIC/Dkk-3 tumor suppressor gene (Ad-REIC) was examined for use in prostate cancer gene therapy. The Ad-human (h) and mouse (m) REIC were previously demonstrated to induce strong anti-cancer effects in vitro and in vivo, and we herein report the results of two in vivo studies. First, intra-tumor Ad-hREIC administration was examined for toxicity and therapeutic effects in a subcutaneous tumor model using the PC3 prostate cancer cell line. Second, intra-prostatic Ad-mREIC administration was tested for toxicity in normal mice. The whole-body and spleen weights, hematological and serum chemistry parameters, and histological evaluation of tissues from throughout the body were analyzed. Both experiments indicated that there was no significant difference in the examined parameters between the Ad-REIC-treated group and the control (PBS- or Ad-LacZ-treated) group. In the in vitro analysis using PC3 cells, a significant apoptotic effect was observed after Ad-hREIC treatment. Confirming this observation, the robust anti-tumor efficacy of Ad-hREIC was demonstrated in the in vivo subcutaneous prostate cancer model. Based on the results of these preclinical experiments, we consider the adenovirus-mediated REIC/Dkk-3 in situ gene therapy to be safe and useful for the clinical treatment of prostate cancer. |
Keywords | REIC Dickkopf-3 gene therapy prostate cancer preclinical study |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2012-02 |
Volume | volume66 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 7 |
End Page | 16 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 22358134 |
Web of Science KeyUT | 000300800700002 |
JaLCDOI | 10.18926/AMO/52140 |
---|---|
FullText URL | 68_1_23.pdf |
Author | Ueno, Tsuyoshi| Toyooka, Shinichi| Fukazawa, Takuya| Kubo, Takafumi| Soh, Junichi| Asano, Hiroaki| Muraoka, Takayuki| Tanaka, Norimitsu| Maki, Yuho| Shien, Kazuhiko| Furukawa, Masashi| Sakaguchi, Masakiyo| Yamamoto, Hiromasa| Tsukuda, Kazunori| Miyoshi, Shinichiro| |
Abstract | The microRNA-34s (miR-34s) have p53 response elements in their 5ʼ-flanking regions and demonstrate tumor-suppressive functions. In malignant pleural mesothelioma (MPM), we previously reported that expression of miR-34b and miR-34c (miR-34b/c) was frequently downregulated by methylation in MPM cell lines and primary tumors. The forced overexpression of miR-34b/c showed significant antitumor effects with the induction of apoptosis in MPM cells. In this study, we examined the in vivo antitumor effects of miR-34b/c using adenovirus vector on MPM. We subcutaneously transplanted NCI-H290, a human MPM cell line, into BALB/C mice and injected adenovirus vector expressing miR-34b/c, luciferase driven by the cytomegalovirus promoter (Ad-miR-34b/c or Ad-Luc), or PBS control into tumors over 5mm in diameter. A statistically significant growth inhibition of the tumor volume was observed in the Ad-miR-34b/c group from day 6 onward compared to the Ad-Luc group. The inhibition rate of Ad-miR-34b/c, compared to the tumor volume treated with Ad-Luc, was 58.6% on day 10 and 54.7% on day13. Our results indicate that adenovirus-mediated miR-34b/c gene therapy could be useful for the clinical treatment of MPM. |
Keywords | mesothelioma microRNA microRNA-34b/c p53 |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2014-02 |
Volume | volume68 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 23 |
End Page | 26 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2014 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 24553485 |
Web of Science KeyUT | 000331592800004 |
JaLCDOI | 10.18926/AMO/60371 |
---|---|
FullText URL | 74_4_327.pdf |
Author | Yamamoto, Ken-ichi| Kagawa, Hiroko| Arimoto, Sakae| Tan, Xian Wen| Yasui, Kento| Oshiki, Toshiyuki| Sakaguchi, Masakiyo| |
Abstract | An increasing accumulation of microplastics and further degraded nanoplastics in our environment is suspected to have harmful effects on humans and animals. To clarify this problem, we tested the cytotoxicity of two types of plastic wrap on human cultured liver cells and mouse primary cultured liver cells. Alcohol extracts from plastic wrap, i.e., polyvinylidene chloride (PVDC), showed cytotoxic effects on the cells. Alcohol extracts of polyethylene (PE) wrap were not toxic. The commercially available PVDC wrap consists of vinylidene chloride, epoxidized soybean oil, epoxidized linseed oil as a stiffener and stabilizer; we sought to identify which component(s) are toxic. The epoxidized soybean oil and epoxidized linseed oil exerted strong cytotoxicity, but the plastic raw material itself, vinylidene chloride, did not. Our findings indicate that plastic wraps should be used with caution in order to prevent health risks. |
Keywords | plastic wrap plasticizer, cytotoxicity, liver cells in vitro |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2020-08 |
Volume | volume74 |
Issue | issue4 |
Publisher | Okayama University Medical School |
Start Page | 327 |
End Page | 334 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2020 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 32843764 |
Web of Science KeyUT | 000562508700008 |
NAID | 120006880210 |
Author | 阪口 政清| |
---|---|
Published Date | 2001-03-25 |
Publication Title | |
Content Type | Thesis or Dissertation |
Author | Ochiai, Kazuhiko| Watanabe, Masami| Ueki, Hideo| Huang, Peng| Fujii, Yasuyuki| Nasu, Yasutomo| Noguchi, Hirofumi| Hirata, Takeshi| Sakaguchi, Masakiyo| Huh, Nam-ho| Kashiwakura, Yuji| Kaku, Haruki| Kumon, Hiromi| |
---|---|
Published Date | 2011-08-26 |
Publication Title | Biochemical and Biophysical Research Communications |
Volume | volume412 |
Issue | issue2 |
Content Type | Journal Article |
Author | Sakaguchi, Masakiyo| Kataoka, Ken| Abarzua, Fernando| Tanimoto, Ryuta| Watanabe, Masami| Murata, Hitoshi| Than, Swe Swe| Kurose, Kaoru| Kashiwakura, Yuji| Ochiai, Kazuhiko| Nasu, Yasutomo| Kumon, Hiromi| Huh, Nam-ho| |
---|---|
Published Date | 2009-05-22 |
Publication Title | The Journal of Biological Chemistry |
Volume | volume284 |
Issue | issue21 |
Content Type | Journal Article |
Author | Tanimoto, Ryuta| Sakaguchi, Masakiyo| Abarzua, Fernando| Kataoka, Ken| Kurose, Kaoru| Murata, Hitoshi| Nasu, Yasutomo| Kumon, Hiromi| Huh, Nam-Ho| |
---|---|
Published Date | 2010-04-01 |
Publication Title | International Journal of Cancer |
Volume | volume126 |
Issue | issue7 |
Content Type | Journal Article |
Author | Putranto, Endy Widya| Murata, Hitoshi| Yamamoto, Ken-Ichi| Kataoka, Ken| Yamada, Hidenori| Futami, Jun-Ichiro| Sakaguchi, Masakiyo| Huh, Nam-Ho| |
---|---|
Published Date | 2013-10 |
Publication Title | International Journal of Molecular Medicine |
Volume | volume32 |
Issue | issue4 |
Content Type | Journal Article |
FullText URL | fulltext.pdf |
---|---|
Author | Chen, Youyi| Sumardika, I Wayan| Tomonobu, Nahoko| Kinoshita, Rie| Inoue, Yusuke| Iioka, Hidekazu| Mitsui, Yosuke| Saito, Ken| Ruma, I Made Winarsa| Sato, Hiroki| Yamauchi, Akira| Murata, Hitoshi| Yamamoto, Ken-ichi| Tomida, Shuta| Shien, Kazuhiko| Yamamoto, Hiromasa| Soh, Junichi| Futami, Junichiro| Kubo, Miyoko| Putranto, Endy Widya| Murakami, Takashi| Liu, Ming| Hibino, Toshihiko| Nishibori, Masahiro| Kondo, Eisaku| Toyooka, Shinichi| Sakaguchi, Masakiyo| |
Published Date | 2019-07 |
Publication Title | Neoplasia |
Volume | volume21 |
Issue | issue7 |
Start Page | 627 |
End Page | 640 |
ISSN | 1522-8002 |
NCID | AA11470191 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2019 The Authors. |
File Version | publisher |
PubMed ID | 31100639 |
DOI | 10.1016/j.neo.2019.04.006 |
Web of Science KeyUT | 000472189700001 |
Related Url | isVersionOf https://doi.org/10.1016/j.neo.2019.04.006 |
FullText URL | fulltext.pdf |
---|---|
Author | Tomonobu, Nahoko| Kinoshita, Rie| Sumardika, I. Wayan| Chen, Youyi| Inoue, Yusuke| Yamauchi, Akira| Yamamoto, Ken-ichi| Murata, Hitoshi| Sakaguchi, Masakiyo| |
Keywords | Melanocytes Melanoma Metastasis Primary culture |
Published Date | 2019-07 |
Publication Title | Biochemistry and Biophysics Reports |
Volume | volume18 |
Publisher | Elsevier |
Start Page | 100619 |
ISSN | 24055808 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2019 The Authors. |
File Version | publisher |
PubMed ID | 30899801 |
DOI | 10.1016/j.bbrep.2019.100619 |
Related Url | isVersionOf https://doi.org/10.1016/j.bbrep.2019.100619 |
FullText URL | fulltext.pdf |
---|---|
Author | Saito, Ken| Iioka, Hidekazu| Maruyama, Satoshi| Sumardika, I. Wayan| Sakaguchi, Masakiyo| Kondo, Eisaku| |
Published Date | 2019-12-31 |
Publication Title | Neoplasia |
Volume | volume21 |
Issue | issue12 |
Publisher | Elsevier |
Start Page | 1121 |
End Page | 1132 |
ISSN | 14765586 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2019 The Authors. Published by Elsevier Inc. on behalf of Neoplasia Press, Inc. |
File Version | publisher |
PubMed ID | 31759250 |
DOI | 10.1016/j.neo.2019.09.003 |
Web of Science KeyUT | 000500825600001 |
Related Url | isVersionOf https://doi.org/10.1016/j.neo.2019.09.003 |
FullText URL | fulltext.pdf |
---|---|
Author | Ueki, Yushi| Saito, Ken| Iioka, Hidekazu| Sakamoto, Izumi| Kanda, Yasuhiro| Sakaguchi, Masakiyo| Horii, Arata| Kondo, Eisaku| |
Keywords | Cancer Molecular Biology |
Published Date | 2020-01-18 |
Publication Title | iScience |
Volume | volume23 |
Issue | issue2 |
Publisher | Cell Press |
Start Page | 100850 |
ISSN | 25890042 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 The Author(s). |
File Version | publisher |
PubMed ID | 32058962 |
DOI | 10.1016/j.isci.2020.100850 |
Web of Science KeyUT | 000518637100047 |
Related Url | isVersionOf https://doi.org/10.1016/j.isci.2020.100850 |
FullText URL | fulltext.pdf |
---|---|
Author | Tomonobu, Nahoko| Komalasari, Ni Luh Gede Yoni| Sumardika, I Wayan| Jiang, Fan| Chen, Youyi| Yamamoto, Ken-ichi| Kinoshita, Rie| Murata, Hitoshi| Inoue, Yusuke| Sakaguchi, Masakiyo| |
Keywords | Xylitol Cancer Glutathione ER stress Chemotherapy |
Published Date | 2020-06-01 |
Publication Title | Chemico-Biological Interactions |
Volume | volume324 |
Publisher | Elsevier |
Start Page | 109085 |
ISSN | 0009-2797 |
NCID | AA0060252X |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 The Authors. |
File Version | publisher |
PubMed ID | 32275922 |
DOI | 10.1016/j.cbi.2020.109085 |
Web of Science KeyUT | 000531490600009 |
Related Url | isVersionOf https://doi.org/10.1016/j.cbi.2020.109085 |
FullText URL | fulltext.pdf |
---|---|
Author | Tomonobu, Nahoko| Kinoshita, Rie| Sakaguchi, Masakiyo| |
Published Date | 2020-04-30 |
Publication Title | Translational Oncology |
Volume | volume13 |
Issue | issue4 |
Publisher | Elsevier |
Start Page | 100753 |
ISSN | 19365233 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 The Authors. Published by Elsevier B.V. |
File Version | publisher |
PubMed ID | 32193075 |
DOI | 10.1016/j.tranon.2020.100753 |
Related Url | isVersionOf https://doi.org/10.1016/j.tranon.2020.100753 |
FullText URL | fulltext.pdf |
---|---|
Author | Gao, Shangze| Wake, Hidenori| Sakaguchi, Masakiyo| Wang, Dengli| Takahashi, Youhei| Teshigawara, Kiyoshi| Zhong, Hui| Mori, Shuji| Liu, Keyue| Takahashi, Hideo| Nishibori, Masahiro| |
Published Date | 2020-06-26 |
Publication Title | iScience |
Volume | volume23 |
Issue | issue6 |
Publisher | Cell Press |
Start Page | 101180 |
ISSN | 2589-0042 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 The Authors. |
File Version | publisher |
PubMed ID | 32498020 |
DOI | 10.1016/j.isci.2020.101180 |
Web of Science KeyUT | 000548236600006 |
Related Url | isVersionOf https://doi.org/10.1016/j.isci.2020.101180 |
FullText URL | fulltext.pdf |
---|---|
Author | Bajkowska, Karolina| Sumardika, I. Wayan| Tomonobu, Nahoko| Chen, Youyi| Yamamoto, Ken-ichi| Kinoshita, Rie| Murata, Hitoshi| Gede Yoni Komalasari, Ni Luh| Jiang, Fan| Yamauchi, Akira| Winarsa Ruma, I. Made| Kasano-Camones, Carlos Ichiro| Inoue, Yusuke| Sakaguchi, Masakiyo| |
Keywords | Lung cancer Metastasis Epithelial-mesenchymal transition Solute carrier family 22 member 18 antisense S100A8/A9 Neuroplastin |
Published Date | 2020-07 |
Publication Title | Biochemistry and Biophysics Reports |
Volume | volume22 |
Publisher | Elsevier |
Start Page | 100768 |
ISSN | 24055808 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 The Authors |
File Version | publisher |
PubMed ID | 32490214 |
DOI | 10.1016/j.bbrep.2020.100768 |
Related Url | isVersionOf https://doi.org/10.1016/j.bbrep.2020.100768 |
FullText URL | fulltext20210630-1.pdf |
---|---|
Author | Nakamura, Kazufumi| Akagi, Satoshi| Ejiri, Kentaro| Yoshida, Masashi| Miyoshi, Toru| Sakaguchi, Masakiyo| Amioka, Naofumi| Suastika, Luh Oliva Saraswati| Kondo, Megumi| Nakayama, Rie| Takaya, Yoichi| Higashimoto, Yuichiro| Fukami, Kei| Matsubara, Hiromi| Ito, Hiroshi| |
Keywords | pulmonary artery smooth muscle cells RAGE aptamer |
Note | This is an Accepted Manuscript published by Elsevier. ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/| |
Published Date | 2020-12-30 |
Publication Title | Journal of Cardiology |
Volume | volume78 |
Issue | issue1 |
Publisher | Elsevier |
Start Page | 12 |
End Page | 16 |
ISSN | 09145087 |
NCID | AN10070473 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2020 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved. |
File Version | author |
PubMed ID | 33386219 |
DOI | 10.1016/j.jjcc.2020.12.009 |
Web of Science KeyUT | 000661421700002 |
Related Url | isVersionOf https://doi.org/10.1016/j.jjcc.2020.12.009 |
FullText URL | fulltext.pdf |
---|---|
Author | He, Fang| Matsumoto, Yoshinori| Asano, Yosuke| Yamamura, Yuriko| Katsuyama, Takayuki| La Rose, Jose| Tomonobu, Nahoko| Komalasari, Ni Luh Gede Yoni| Sakaguchi, Masakiyo| Rottapel, Robert| Wada, Jun| |
Keywords | ABL Abelson murine leukemia viral oncogene homolog Runx2 (runt-related transcription factor 2) tyrosine phosphorylation invasion |
Note | Erratum in https://ousar.lib.okayama-u.ac.jp/62331| |
Published Date | 2021-05-31 |
Publication Title | Frontiers In Oncology |
Volume | volume11 |
Publisher | Frontiers Media S.A. |
Start Page | 665273 |
ISSN | 2234-943X |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2021 He, Matsumoto, Asano, Yamamura, Katsuyama, La Rose, Tomonobu, Komalasari, Sakaguchi, Rottapel and Wada. |
File Version | publisher |
PubMed ID | 34136397 |
NAID | 120007089841 |
DOI | 10.3389/fonc.2021.665273 |
Web of Science KeyUT | 000661158800001 |
Related Url | isVersionOf https://doi.org/10.3389/fonc.2021.665273 |