ID | 67183 |
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Mu, Xindi
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ono, Mitsuaki
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Nguyen, Ha Thi Thu
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Wang, Ziyi
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Zhao, Kun
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Komori, Taishi
Yonezawa, Tomoko
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Kuboki, Takuo
Department of Oral Rehabilitation and Implantology, Okayama University Hospital
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Oohashi, Toshitaka
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abstract | The oral mucosa functions as a physico-chemical and immune barrier to external stimuli, and an adequate width of the keratinized mucosa around the teeth or implants is crucial to maintaining them in a healthy and stable condition. In this study, for the first time, bulk RNA-seq analysis was performed to explore the gene expression of laser microdissected epithelium and lamina propria from mice, aiming to investigate the differences between keratinized and non-keratinized oral mucosa. Based on the differentially expressed genes (DEGs) and Gene Ontology (GO) Enrichment Analysis, bone morphogenetic protein 2 (BMP-2) was identified to be a potential regulator of oral mucosal keratinization. Monoculture and epithelial-mesenchymal cell co-culture models in the air-liquid interface (ALI) indicated that BMP-2 has direct and positive effects on epithelial keratinization and proliferation. We further performed bulk RNA-seq of the ALI monoculture stimulated with BMP-2 in an attempt to identify the downstream factors promoting epithelial keratinization and proliferation. Analysis of the DEGs identified, among others, IGF2, ID1, LTBP1, LOX, SERPINE1, IL24, and MMP1 as key factors. In summary, these results revealed the involvement of a well-known growth factor responsible for bone development, BMP-2, in the mechanism of oral mucosal keratinization and proliferation, and pointed out the possible downstream genes involved in this mechanism.
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Keywords | cell differentiation
epithelia
growth factor(s)
bioinformatics
extracellular matrix (ECM)
mucocutaneous disorders
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Published Date | 2024-05-09
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Publication Title |
Cells
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Volume | volume13
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Issue | issue10
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Publisher | MDPI
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Start Page | 807
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ISSN | 2073-4409
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2024 by the authors.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.3390/cells13100807
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Mu, X.; Ono, M.; Nguyen, H.T.T.; Wang, Z.; Zhao, K.; Komori, T.; Yonezawa, T.; Kuboki, T.; Oohashi, T. Exploring the Regulators of Keratinization: Role of BMP-2 in Oral Mucosa. Cells 2024, 13, 807. https://doi.org/10.3390/cells13100807
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP22K10100
JP22H03280
JP19H03841
JP22KF0265
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