ID | 67183 |
フルテキストURL | |
著者 |
Mu, Xindi
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ono, Mitsuaki
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Nguyen, Ha Thi Thu
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Wang, Ziyi
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Zhao, Kun
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Komori, Taishi
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yonezawa, Tomoko
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Kuboki, Takuo
Department of Oral Rehabilitation and Implantology, Okayama University Hospital
ORCID
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Oohashi, Toshitaka
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | The oral mucosa functions as a physico-chemical and immune barrier to external stimuli, and an adequate width of the keratinized mucosa around the teeth or implants is crucial to maintaining them in a healthy and stable condition. In this study, for the first time, bulk RNA-seq analysis was performed to explore the gene expression of laser microdissected epithelium and lamina propria from mice, aiming to investigate the differences between keratinized and non-keratinized oral mucosa. Based on the differentially expressed genes (DEGs) and Gene Ontology (GO) Enrichment Analysis, bone morphogenetic protein 2 (BMP-2) was identified to be a potential regulator of oral mucosal keratinization. Monoculture and epithelial-mesenchymal cell co-culture models in the air-liquid interface (ALI) indicated that BMP-2 has direct and positive effects on epithelial keratinization and proliferation. We further performed bulk RNA-seq of the ALI monoculture stimulated with BMP-2 in an attempt to identify the downstream factors promoting epithelial keratinization and proliferation. Analysis of the DEGs identified, among others, IGF2, ID1, LTBP1, LOX, SERPINE1, IL24, and MMP1 as key factors. In summary, these results revealed the involvement of a well-known growth factor responsible for bone development, BMP-2, in the mechanism of oral mucosal keratinization and proliferation, and pointed out the possible downstream genes involved in this mechanism.
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キーワード | cell differentiation
epithelia
growth factor(s)
bioinformatics
extracellular matrix (ECM)
mucocutaneous disorders
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発行日 | 2024-05-09
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出版物タイトル |
Cells
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巻 | 13巻
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号 | 10号
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出版者 | MDPI
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開始ページ | 807
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ISSN | 2073-4409
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2024 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/cells13100807
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Mu, X.; Ono, M.; Nguyen, H.T.T.; Wang, Z.; Zhao, K.; Komori, T.; Yonezawa, T.; Kuboki, T.; Oohashi, T. Exploring the Regulators of Keratinization: Role of BMP-2 in Oral Mucosa. Cells 2024, 13, 807. https://doi.org/10.3390/cells13100807
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | JP22K10100
JP22H03280
JP19H03841
JP22KF0265
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