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ID 65943
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Ono-Minagi, Hitomi Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nohno, Tsutomu Department of Cytology and Histology, Okayama University Medical School
Serizawa, Takashi Department of Physiology, Keio University School of Medicine
Usami, Yu Department of Oral and Maxillofacial Pathology, Osaka University Graduate School of Dentistry
Sakai, Takayoshi Department of Rehabilitation for Orofacial Disorders, Osaka University Graduate School of Dentistry
Okano, Hideyuki Department of Physiology, Keio University School of Medicine
Ohuchi, Hideyo Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap
Abstract
The vertebrate body comprises four distinct cell populations: cells derived from (1) ectoderm, (2) mesoderm, (3) endoderm, and (4) neural crest cells, often referred to as the fourth germ layer. Neural crest cells arise when the neural plate edges fuse to form a neural tube, which eventually develops into the brain and spinal cord. To date, the embryonic origin of exocrine glands located in the head and neck remains under debate. In this study, transgenic TRiCK mice were used to investigate the germinal origin of the salivary and lacrimal glands. TRiCK mice express fluorescent proteins under the regulatory control of Sox1, T/Brachyury, and Sox17 gene expressions. These genes are representative marker genes for neuroectoderm (Sox1), mesoderm (T), and endoderm (Sox17). Using this approach, the cellular lineages of the salivary and lacrimal glands were examined. We demonstrate that the salivary and lacrimal glands contain cells derived from all three germ layers. Notably, a subset of Sox1-driven fluorescent cells differentiated into epithelial cells, implying their neural crest origin. Also, these Sox1-driven fluorescent cells expressed high levels of stem cell markers. These cells were particularly pronounced in duct ligation and wound damage models, suggesting the involvement of neural crest-derived epithelial cells in regenerative processes following tissue injury. This study provides compelling evidence clarifying the germinal origin of exocrine glands and the contribution of neural crest-derived cells within the glandular epithelium to the regenerative response following tissue damage.
Keywords
salivary and lacrimal glands
development
three germ layers
neural crest
Published Date
2023-09-05
Publication Title
International Journal of Molecular Sciences
Volume
volume24
Issue
issue18
Publisher
MDPI
Start Page
13692
ISSN
1661-6596
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
Copyright Holders
© 2023 by the authors.
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isVersionOf https://doi.org/10.3390/ijms241813692
License
https://creativecommons.org/licenses/by/4.0/
Citation
Ono-Minagi, H.; Nohno, T.; Serizawa, T.; Usami, Y.; Sakai, T.; Okano, H.; Ohuchi, H. The Germinal Origin of Salivary and Lacrimal Glands and the Contributions of Neural Crest Cell-Derived Epithelium to Tissue Regeneration. Int. J. Mol. Sci. 2023, 24, 13692. https://doi.org/10.3390/ijms241813692
Funder Name
Japan Society for the Promotion of Science
助成番号
JP21K10093
JP21J40187