ID | 65943 |
フルテキストURL | |
著者 |
Ono-Minagi, Hitomi
Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nohno, Tsutomu
Department of Cytology and Histology, Okayama University Medical School
Serizawa, Takashi
Department of Physiology, Keio University School of Medicine
Usami, Yu
Department of Oral and Maxillofacial Pathology, Osaka University Graduate School of Dentistry
Sakai, Takayoshi
Department of Rehabilitation for Orofacial Disorders, Osaka University Graduate School of Dentistry
Okano, Hideyuki
Department of Physiology, Keio University School of Medicine
Ohuchi, Hideyo
Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Kaken ID
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抄録 | The vertebrate body comprises four distinct cell populations: cells derived from (1) ectoderm, (2) mesoderm, (3) endoderm, and (4) neural crest cells, often referred to as the fourth germ layer. Neural crest cells arise when the neural plate edges fuse to form a neural tube, which eventually develops into the brain and spinal cord. To date, the embryonic origin of exocrine glands located in the head and neck remains under debate. In this study, transgenic TRiCK mice were used to investigate the germinal origin of the salivary and lacrimal glands. TRiCK mice express fluorescent proteins under the regulatory control of Sox1, T/Brachyury, and Sox17 gene expressions. These genes are representative marker genes for neuroectoderm (Sox1), mesoderm (T), and endoderm (Sox17). Using this approach, the cellular lineages of the salivary and lacrimal glands were examined. We demonstrate that the salivary and lacrimal glands contain cells derived from all three germ layers. Notably, a subset of Sox1-driven fluorescent cells differentiated into epithelial cells, implying their neural crest origin. Also, these Sox1-driven fluorescent cells expressed high levels of stem cell markers. These cells were particularly pronounced in duct ligation and wound damage models, suggesting the involvement of neural crest-derived epithelial cells in regenerative processes following tissue injury. This study provides compelling evidence clarifying the germinal origin of exocrine glands and the contribution of neural crest-derived cells within the glandular epithelium to the regenerative response following tissue damage.
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キーワード | salivary and lacrimal glands
development
three germ layers
neural crest
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発行日 | 2023-09-05
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出版物タイトル |
International Journal of Molecular Sciences
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巻 | 24巻
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号 | 18号
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出版者 | MDPI
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開始ページ | 13692
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ISSN | 1661-6596
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2023 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.3390/ijms241813692
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Ono-Minagi, H.; Nohno, T.; Serizawa, T.; Usami, Y.; Sakai, T.; Okano, H.; Ohuchi, H. The Germinal Origin of Salivary and Lacrimal Glands and the Contributions of Neural Crest Cell-Derived Epithelium to Tissue Regeneration. Int. J. Mol. Sci. 2023, 24, 13692. https://doi.org/10.3390/ijms241813692
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | JP21K10093
JP21J40187
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